Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02240 and BWI76_RS02245 overlap by 6 nucleotides BWI76_RS02245 and BWI76_RS02250 are separated by 330 nucleotides BWI76_RS02250 and BWI76_RS02255 are separated by 121 nucleotides BWI76_RS02255 and BWI76_RS02260 overlap by 1 nucleotides
BWI76_RS02240: BWI76_RS02240 - alkylphosphonate utilization operon protein PhnA, at 461,980 to 462,315
_RS02240
BWI76_RS02245: BWI76_RS02245 - hypothetical protein, at 462,310 to 462,525
_RS02245
BWI76_RS02250: BWI76_RS02250 - proline/betaine transporter, at 462,856 to 464,358
_RS02250
BWI76_RS02255: BWI76_RS02255 - chaperone-modulator protein CbpM, at 464,480 to 464,785
_RS02255
BWI76_RS02260: BWI76_RS02260 - DNA-binding protein, at 464,785 to 465,705
_RS02260
Position (kb) 462
463
464
465 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 461.861 kb on - strand at 461.861 kb on - strand at 461.895 kb on + strand at 461.895 kb on + strand at 461.900 kb on + strand at 461.901 kb on - strand at 461.908 kb on - strand at 461.914 kb on + strand at 461.919 kb on + strand at 461.919 kb on + strand at 461.921 kb on + strand at 461.921 kb on + strand at 461.927 kb on - strand at 462.021 kb on - strand, within BWI76_RS02240 at 462.040 kb on - strand, within BWI76_RS02240 at 462.055 kb on + strand at 462.056 kb on + strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.073 kb on + strand, within BWI76_RS02240 at 462.102 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.113 kb on + strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.144 kb on + strand, within BWI76_RS02240 at 462.202 kb on + strand, within BWI76_RS02240 at 462.204 kb on + strand, within BWI76_RS02240 at 462.205 kb on + strand, within BWI76_RS02240 at 462.212 kb on + strand, within BWI76_RS02240 at 462.213 kb on + strand, within BWI76_RS02240 at 462.218 kb on + strand, within BWI76_RS02240 at 462.220 kb on - strand, within BWI76_RS02240 at 462.221 kb on - strand, within BWI76_RS02240 at 462.223 kb on + strand, within BWI76_RS02240 at 462.226 kb on - strand, within BWI76_RS02240 at 462.226 kb on - strand at 462.228 kb on + strand, within BWI76_RS02240 at 462.260 kb on - strand, within BWI76_RS02240 at 462.273 kb on - strand, within BWI76_RS02240 at 462.274 kb on + strand, within BWI76_RS02240 at 462.299 kb on + strand at 462.317 kb on - strand at 462.343 kb on - strand, within BWI76_RS02245 at 462.343 kb on - strand, within BWI76_RS02245 at 462.376 kb on - strand, within BWI76_RS02245 at 462.380 kb on + strand, within BWI76_RS02245 at 462.382 kb on - strand, within BWI76_RS02245 at 462.435 kb on + strand, within BWI76_RS02245 at 462.443 kb on - strand, within BWI76_RS02245 at 462.443 kb on - strand, within BWI76_RS02245 at 462.443 kb on - strand, within BWI76_RS02245 at 462.514 kb on + strand at 462.526 kb on + strand at 462.558 kb on - strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.566 kb on + strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.574 kb on - strand at 462.613 kb on + strand at 462.613 kb on + strand at 462.656 kb on - strand at 462.664 kb on + strand at 462.680 kb on + strand at 462.680 kb on + strand at 462.683 kb on - strand at 462.685 kb on + strand at 462.685 kb on + strand at 462.685 kb on + strand at 462.685 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.690 kb on + strand at 462.693 kb on + strand at 462.693 kb on - strand at 462.693 kb on - strand at 462.693 kb on - strand at 462.693 kb on - strand at 462.698 kb on - strand at 462.698 kb on - strand at 462.699 kb on + strand at 462.706 kb on + strand at 462.708 kb on + strand at 462.714 kb on - strand at 462.718 kb on + strand at 462.727 kb on - strand at 462.727 kb on - strand at 462.729 kb on + strand at 462.729 kb on + strand at 462.729 kb on + strand at 462.733 kb on + strand at 462.733 kb on + strand at 462.734 kb on + strand at 462.737 kb on - strand at 462.737 kb on - strand at 462.746 kb on - strand at 462.749 kb on + strand at 462.755 kb on + strand at 462.760 kb on + strand at 462.763 kb on - strand at 462.765 kb on + strand at 462.793 kb on - strand at 462.802 kb on - strand at 462.813 kb on + strand at 462.841 kb on - strand at 462.889 kb on + strand at 462.951 kb on + strand at 462.951 kb on + strand at 463.008 kb on + strand, within BWI76_RS02250 at 463.023 kb on + strand, within BWI76_RS02250 at 463.050 kb on - strand, within BWI76_RS02250 at 463.050 kb on - strand, within BWI76_RS02250 at 463.059 kb on + strand, within BWI76_RS02250 at 463.117 kb on + strand, within BWI76_RS02250 at 463.259 kb on - strand, within BWI76_RS02250 at 463.276 kb on - strand, within BWI76_RS02250 at 463.285 kb on + strand, within BWI76_RS02250 at 463.285 kb on + strand, within BWI76_RS02250 at 463.293 kb on - strand, within BWI76_RS02250 at 463.325 kb on - strand, within BWI76_RS02250 at 463.379 kb on + strand, within BWI76_RS02250 at 463.454 kb on + strand, within BWI76_RS02250 at 463.584 kb on - strand, within BWI76_RS02250 at 463.609 kb on - strand, within BWI76_RS02250 at 463.626 kb on - strand, within BWI76_RS02250 at 463.646 kb on + strand, within BWI76_RS02250 at 463.730 kb on + strand, within BWI76_RS02250 at 463.738 kb on - strand, within BWI76_RS02250 at 463.840 kb on - strand, within BWI76_RS02250 at 463.999 kb on + strand, within BWI76_RS02250 at 464.047 kb on - strand, within BWI76_RS02250 at 464.069 kb on + strand, within BWI76_RS02250 at 464.069 kb on + strand, within BWI76_RS02250 at 464.096 kb on + strand, within BWI76_RS02250 at 464.151 kb on + strand, within BWI76_RS02250 at 464.326 kb on - strand at 464.447 kb on - strand at 464.614 kb on + strand, within BWI76_RS02255 at 464.619 kb on - strand, within BWI76_RS02255 at 464.632 kb on - strand, within BWI76_RS02255 at 464.683 kb on + strand, within BWI76_RS02255 at 464.801 kb on + strand at 464.831 kb on + strand at 464.934 kb on + strand, within BWI76_RS02260 at 464.934 kb on + strand, within BWI76_RS02260 at 465.097 kb on - strand, within BWI76_RS02260 at 465.279 kb on + strand, within BWI76_RS02260
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM remove 461,861 - +1.0 461,861 - +1.1 461,895 + -0.3 461,895 + -1.5 461,900 + +0.4 461,901 - +1.4 461,908 - +0.3 461,914 + -0.6 461,919 + -1.8 461,919 + -1.4 461,921 + +0.1 461,921 + +0.2 461,927 - -1.0 462,021 - BWI76_RS02240 0.12 -0.5 462,040 - BWI76_RS02240 0.18 +0.3 462,055 + +2.5 462,056 + BWI76_RS02240 0.23 -0.3 462,064 - BWI76_RS02240 0.25 +0.2 462,064 - BWI76_RS02240 0.25 -0.4 462,064 - BWI76_RS02240 0.25 +0.0 462,064 - BWI76_RS02240 0.25 -0.2 462,064 - BWI76_RS02240 0.25 -0.0 462,064 - BWI76_RS02240 0.25 +0.4 462,064 - BWI76_RS02240 0.25 +0.0 462,073 + BWI76_RS02240 0.28 -0.3 462,102 + BWI76_RS02240 0.36 +1.4 462,112 + BWI76_RS02240 0.39 -1.4 462,112 + BWI76_RS02240 0.39 -0.0 462,113 + BWI76_RS02240 0.40 +0.8 462,120 - BWI76_RS02240 0.42 +0.5 462,120 - BWI76_RS02240 0.42 -1.2 462,120 - BWI76_RS02240 0.42 -0.2 462,121 - BWI76_RS02240 0.42 +0.3 462,121 - BWI76_RS02240 0.42 +0.4 462,144 + BWI76_RS02240 0.49 -1.8 462,202 + BWI76_RS02240 0.66 -0.2 462,204 + BWI76_RS02240 0.67 +0.7 462,205 + BWI76_RS02240 0.67 +0.3 462,212 + BWI76_RS02240 0.69 +0.1 462,213 + BWI76_RS02240 0.69 -0.2 462,218 + BWI76_RS02240 0.71 -1.5 462,220 - BWI76_RS02240 0.71 -0.2 462,221 - BWI76_RS02240 0.72 +0.2 462,223 + BWI76_RS02240 0.72 +0.2 462,226 - BWI76_RS02240 0.73 +0.0 462,226 - -0.2 462,228 + BWI76_RS02240 0.74 +0.5 462,260 - BWI76_RS02240 0.83 -0.5 462,273 - BWI76_RS02240 0.87 +0.6 462,274 + BWI76_RS02240 0.88 +2.1 462,299 + -0.5 462,317 - -0.6 462,343 - BWI76_RS02245 0.15 +1.8 462,343 - BWI76_RS02245 0.15 -0.3 462,376 - BWI76_RS02245 0.31 +0.3 462,380 + BWI76_RS02245 0.32 -0.4 462,382 - BWI76_RS02245 0.33 +0.1 462,435 + BWI76_RS02245 0.58 -0.7 462,443 - BWI76_RS02245 0.62 -1.4 462,443 - BWI76_RS02245 0.62 -0.1 462,443 - BWI76_RS02245 0.62 -0.7 462,514 + -0.8 462,526 + -0.2 462,558 - -1.0 462,566 + -1.2 462,566 + +0.5 462,566 + +0.1 462,566 + +0.5 462,566 + +0.4 462,566 + +0.1 462,566 + -3.3 462,566 + +0.0 462,566 + +2.4 462,566 + +0.4 462,566 + -0.4 462,566 + +0.3 462,566 + +0.7 462,566 + -0.1 462,566 + -1.0 462,566 + +0.7 462,566 + +0.7 462,566 + +0.4 462,566 + -1.0 462,574 - -0.1 462,574 - +1.1 462,574 - +1.0 462,574 - +0.8 462,574 - +0.3 462,574 - -0.6 462,574 - -0.4 462,574 - -1.4 462,574 - +0.9 462,574 - -0.9 462,574 - +0.5 462,574 - -0.5 462,574 - +2.0 462,574 - +0.6 462,574 - +0.4 462,574 - +0.1 462,613 + +0.3 462,613 + -0.6 462,656 - -1.8 462,664 + +0.2 462,680 + -0.1 462,680 + -3.2 462,683 - -1.4 462,685 + -0.1 462,685 + -0.2 462,685 + -2.2 462,685 + -0.0 462,690 + +1.4 462,690 + +0.2 462,690 + +0.4 462,690 + +0.1 462,690 + +1.0 462,690 + +0.3 462,690 + +1.0 462,690 + +0.4 462,690 + +0.6 462,690 + -0.7 462,690 + +1.4 462,693 + +0.6 462,693 - -2.1 462,693 - -1.2 462,693 - -0.9 462,693 - -2.0 462,698 - -0.7 462,698 - -1.3 462,699 + +0.1 462,706 + -1.0 462,708 + +1.0 462,714 - -1.3 462,718 + -0.2 462,727 - +1.0 462,727 - -1.9 462,729 + +0.7 462,729 + +0.7 462,729 + +0.6 462,733 + -2.2 462,733 + -0.1 462,734 + +0.8 462,737 - +1.6 462,737 - +1.6 462,746 - +0.6 462,749 + +1.4 462,755 + +0.6 462,760 + +0.3 462,763 - -2.1 462,765 + +0.4 462,793 - -1.1 462,802 - -2.5 462,813 + +0.3 462,841 - -0.2 462,889 + -2.5 462,951 + -2.2 462,951 + -3.3 463,008 + BWI76_RS02250 0.10 -2.0 463,023 + BWI76_RS02250 0.11 -3.9 463,050 - BWI76_RS02250 0.13 -1.7 463,050 - BWI76_RS02250 0.13 -4.2 463,059 + BWI76_RS02250 0.14 -2.3 463,117 + BWI76_RS02250 0.17 -2.9 463,259 - BWI76_RS02250 0.27 +2.0 463,276 - BWI76_RS02250 0.28 -3.9 463,285 + BWI76_RS02250 0.29 -2.8 463,285 + BWI76_RS02250 0.29 -5.4 463,293 - BWI76_RS02250 0.29 -2.2 463,325 - BWI76_RS02250 0.31 -3.5 463,379 + BWI76_RS02250 0.35 -2.8 463,454 + BWI76_RS02250 0.40 -2.8 463,584 - BWI76_RS02250 0.48 -3.2 463,609 - BWI76_RS02250 0.50 -2.8 463,626 - BWI76_RS02250 0.51 -1.9 463,646 + BWI76_RS02250 0.53 -0.6 463,730 + BWI76_RS02250 0.58 -3.2 463,738 - BWI76_RS02250 0.59 -2.3 463,840 - BWI76_RS02250 0.65 -2.8 463,999 + BWI76_RS02250 0.76 -2.5 464,047 - BWI76_RS02250 0.79 -1.6 464,069 + BWI76_RS02250 0.81 -2.8 464,069 + BWI76_RS02250 0.81 -3.0 464,096 + BWI76_RS02250 0.83 -2.9 464,151 + BWI76_RS02250 0.86 +1.4 464,326 - -2.4 464,447 - -0.9 464,614 + BWI76_RS02255 0.44 -0.7 464,619 - BWI76_RS02255 0.45 +1.0 464,632 - BWI76_RS02255 0.50 +0.5 464,683 + BWI76_RS02255 0.66 -0.5 464,801 + -0.4 464,831 + -0.1 464,934 + BWI76_RS02260 0.16 -0.6 464,934 + BWI76_RS02260 0.16 -0.3 465,097 - BWI76_RS02260 0.34 -0.2 465,279 + BWI76_RS02260 0.54 +1.1
Or see this region's nucleotide sequence