Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01570 and BWI76_RS01575 are separated by 9 nucleotides BWI76_RS01575 and BWI76_RS01580 are separated by 42 nucleotides BWI76_RS01580 and BWI76_RS01585 are separated by 186 nucleotides BWI76_RS01585 and BWI76_RS01590 are separated by 12 nucleotides
BWI76_RS01570: BWI76_RS01570 - uroporphyrinogen decarboxylase, at 319,579 to 320,643
_RS01570
BWI76_RS01575: BWI76_RS01575 - endonuclease V, at 320,653 to 321,324
_RS01575
BWI76_RS01580: BWI76_RS01580 - hypothetical protein, at 321,367 to 321,957
_RS01580
BWI76_RS01585: BWI76_RS01585 - transcriptional regulator, at 322,144 to 322,416
_RS01585
BWI76_RS01590: BWI76_RS01590 - hypothetical protein, at 322,429 to 323,121
_RS01590
Position (kb) 321
322 Strain fitness (log2 ratio) -2
-1
0
1 at 320.637 kb on + strand at 320.803 kb on + strand, within BWI76_RS01575 at 320.805 kb on + strand, within BWI76_RS01575 at 320.873 kb on - strand, within BWI76_RS01575 at 320.918 kb on - strand, within BWI76_RS01575 at 321.111 kb on + strand, within BWI76_RS01575 at 321.201 kb on - strand, within BWI76_RS01575 at 321.201 kb on - strand, within BWI76_RS01575 at 321.201 kb on - strand, within BWI76_RS01575 at 321.201 kb on - strand, within BWI76_RS01575 at 321.210 kb on + strand, within BWI76_RS01575 at 321.211 kb on - strand, within BWI76_RS01575 at 321.246 kb on + strand, within BWI76_RS01575 at 321.263 kb on + strand at 321.617 kb on + strand, within BWI76_RS01580 at 321.625 kb on - strand, within BWI76_RS01580 at 321.888 kb on + strand, within BWI76_RS01580 at 322.181 kb on + strand, within BWI76_RS01585 at 322.181 kb on + strand at 322.189 kb on - strand, within BWI76_RS01585 at 322.220 kb on + strand, within BWI76_RS01585 at 322.289 kb on + strand, within BWI76_RS01585 at 322.305 kb on - strand, within BWI76_RS01585 at 322.310 kb on - strand, within BWI76_RS01585 at 322.329 kb on + strand, within BWI76_RS01585 at 322.419 kb on + strand at 322.419 kb on - strand at 322.608 kb on - strand, within BWI76_RS01590 at 322.640 kb on - strand, within BWI76_RS01590 at 322.756 kb on - strand, within BWI76_RS01590 at 322.772 kb on + strand, within BWI76_RS01590 at 322.772 kb on + strand, within BWI76_RS01590 at 322.780 kb on - strand, within BWI76_RS01590 at 322.952 kb on + strand, within BWI76_RS01590
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM remove 320,637 + -0.7 320,803 + BWI76_RS01575 0.22 +0.7 320,805 + BWI76_RS01575 0.23 -0.6 320,873 - BWI76_RS01575 0.33 +0.4 320,918 - BWI76_RS01575 0.39 -0.1 321,111 + BWI76_RS01575 0.68 +0.1 321,201 - BWI76_RS01575 0.82 +0.2 321,201 - BWI76_RS01575 0.82 +0.3 321,201 - BWI76_RS01575 0.82 -0.5 321,201 - BWI76_RS01575 0.82 +0.4 321,210 + BWI76_RS01575 0.83 +0.2 321,211 - BWI76_RS01575 0.83 +0.3 321,246 + BWI76_RS01575 0.88 +1.0 321,263 + +1.2 321,617 + BWI76_RS01580 0.42 -0.4 321,625 - BWI76_RS01580 0.44 -2.1 321,888 + BWI76_RS01580 0.88 -2.7 322,181 + BWI76_RS01585 0.14 -0.1 322,181 + -1.8 322,189 - BWI76_RS01585 0.16 -1.1 322,220 + BWI76_RS01585 0.28 -0.2 322,289 + BWI76_RS01585 0.53 -1.1 322,305 - BWI76_RS01585 0.59 -0.1 322,310 - BWI76_RS01585 0.61 -0.1 322,329 + BWI76_RS01585 0.68 -1.9 322,419 + +0.1 322,419 - +0.8 322,608 - BWI76_RS01590 0.26 -0.3 322,640 - BWI76_RS01590 0.30 +0.1 322,756 - BWI76_RS01590 0.47 +0.7 322,772 + BWI76_RS01590 0.49 -0.7 322,772 + BWI76_RS01590 0.49 +1.5 322,780 - BWI76_RS01590 0.51 -0.4 322,952 + BWI76_RS01590 0.75 +0.9
Or see this region's nucleotide sequence