Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01210 and BWI76_RS01215 overlap by 1 nucleotides BWI76_RS01215 and BWI76_RS01220 are separated by 77 nucleotides BWI76_RS01220 and BWI76_RS01225 are separated by 354 nucleotides
BWI76_RS01210: BWI76_RS01210 - tyrosine recombinase XerC, at 246,885 to 247,787
_RS01210
BWI76_RS01215: BWI76_RS01215 - flavin mononucleotide phosphatase, at 247,787 to 248,503
_RS01215
BWI76_RS01220: BWI76_RS01220 - DNA-dependent helicase II, at 248,581 to 250,743
_RS01220
BWI76_RS01225: BWI76_RS01225 - magnesium transporter CorA, at 251,098 to 252,048
_RS01225
Position (kb) 248
249
250
251 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 247.835 kb on - strand at 247.857 kb on + strand at 247.916 kb on - strand, within BWI76_RS01215 at 248.806 kb on - strand, within BWI76_RS01220 at 248.808 kb on + strand, within BWI76_RS01220 at 248.811 kb on - strand, within BWI76_RS01220 at 248.824 kb on - strand, within BWI76_RS01220 at 248.878 kb on + strand, within BWI76_RS01220 at 249.087 kb on - strand, within BWI76_RS01220 at 249.369 kb on - strand, within BWI76_RS01220 at 249.617 kb on + strand, within BWI76_RS01220 at 249.695 kb on - strand, within BWI76_RS01220 at 249.796 kb on + strand, within BWI76_RS01220 at 249.853 kb on - strand, within BWI76_RS01220 at 250.211 kb on - strand, within BWI76_RS01220 at 250.245 kb on + strand, within BWI76_RS01220 at 250.399 kb on + strand, within BWI76_RS01220 at 250.507 kb on - strand, within BWI76_RS01220 at 250.979 kb on + strand at 251.012 kb on + strand at 251.018 kb on + strand at 251.188 kb on - strand at 251.236 kb on + strand, within BWI76_RS01225 at 251.255 kb on + strand, within BWI76_RS01225 at 251.298 kb on + strand, within BWI76_RS01225 at 251.366 kb on + strand, within BWI76_RS01225 at 251.414 kb on + strand, within BWI76_RS01225 at 251.547 kb on + strand, within BWI76_RS01225
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM remove 247,835 - +0.0 247,857 + +0.3 247,916 - BWI76_RS01215 0.18 -1.2 248,806 - BWI76_RS01220 0.10 -0.6 248,808 + BWI76_RS01220 0.10 -1.1 248,811 - BWI76_RS01220 0.11 -1.5 248,824 - BWI76_RS01220 0.11 -1.0 248,878 + BWI76_RS01220 0.14 -1.1 249,087 - BWI76_RS01220 0.23 +0.1 249,369 - BWI76_RS01220 0.36 -0.5 249,617 + BWI76_RS01220 0.48 -2.2 249,695 - BWI76_RS01220 0.52 -2.9 249,796 + BWI76_RS01220 0.56 -0.8 249,853 - BWI76_RS01220 0.59 -0.9 250,211 - BWI76_RS01220 0.75 -0.9 250,245 + BWI76_RS01220 0.77 +0.3 250,399 + BWI76_RS01220 0.84 -0.7 250,507 - BWI76_RS01220 0.89 +0.2 250,979 + -0.1 251,012 + -0.1 251,018 + +0.0 251,188 - +1.1 251,236 + BWI76_RS01225 0.15 +0.0 251,255 + BWI76_RS01225 0.17 +0.3 251,298 + BWI76_RS01225 0.21 -0.2 251,366 + BWI76_RS01225 0.28 +0.7 251,414 + BWI76_RS01225 0.33 -0.2 251,547 + BWI76_RS01225 0.47 -0.3
Or see this region's nucleotide sequence