Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14855

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14850 and BWI76_RS14855 are separated by 255 nucleotidesBWI76_RS14855 and BWI76_RS14860 are separated by 66 nucleotides BWI76_RS14850: BWI76_RS14850 - LacI family transcriptional regulator, at 3,110,415 to 3,111,446 _RS14850 BWI76_RS14855: BWI76_RS14855 - periplasmic binding protein/LacI transcriptional regulator, at 3,111,702 to 3,112,655 _RS14855 BWI76_RS14860: BWI76_RS14860 - ABC transporter, at 3,112,722 to 3,114,254 _RS14860 Position (kb) 3111 3112 3113Strain fitness (log2 ratio) -1 0 1 2at 3110.826 kb on - strand, within BWI76_RS14850at 3110.991 kb on - strand, within BWI76_RS14850at 3111.013 kb on - strand, within BWI76_RS14850at 3111.266 kb on + strand, within BWI76_RS14850at 3111.274 kb on - strand, within BWI76_RS14850at 3111.330 kb on + strand, within BWI76_RS14850at 3111.372 kb on - strandat 3111.468 kb on + strandat 3111.567 kb on + strandat 3111.651 kb on + strandat 3111.652 kb on + strandat 3111.660 kb on - strandat 3111.660 kb on - strandat 3111.660 kb on - strandat 3111.660 kb on - strandat 3111.842 kb on + strand, within BWI76_RS14855at 3111.844 kb on - strand, within BWI76_RS14855at 3111.907 kb on + strand, within BWI76_RS14855at 3111.972 kb on - strand, within BWI76_RS14855at 3112.231 kb on - strand, within BWI76_RS14855at 3112.435 kb on + strand, within BWI76_RS14855at 3112.527 kb on + strand, within BWI76_RS14855at 3112.554 kb on + strand, within BWI76_RS14855at 3112.712 kb on - strandat 3112.743 kb on + strandat 3112.859 kb on + strandat 3112.859 kb on + strandat 3112.962 kb on - strand, within BWI76_RS14860at 3113.005 kb on - strand, within BWI76_RS14860at 3113.052 kb on - strand, within BWI76_RS14860at 3113.297 kb on - strand, within BWI76_RS14860at 3113.344 kb on - strand, within BWI76_RS14860at 3113.551 kb on + strand, within BWI76_RS14860at 3113.602 kb on - strand, within BWI76_RS14860

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
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3,110,826 - BWI76_RS14850 0.40 -0.2
3,110,991 - BWI76_RS14850 0.56 -0.2
3,111,013 - BWI76_RS14850 0.58 -0.4
3,111,266 + BWI76_RS14850 0.82 +2.0
3,111,274 - BWI76_RS14850 0.83 +0.0
3,111,330 + BWI76_RS14850 0.89 +1.3
3,111,372 - -0.2
3,111,468 + -0.2
3,111,567 + -0.4
3,111,651 + +0.1
3,111,652 + +0.3
3,111,660 - -0.2
3,111,660 - -0.2
3,111,660 - +0.3
3,111,660 - -1.2
3,111,842 + BWI76_RS14855 0.15 +0.4
3,111,844 - BWI76_RS14855 0.15 -0.5
3,111,907 + BWI76_RS14855 0.21 -0.9
3,111,972 - BWI76_RS14855 0.28 -0.7
3,112,231 - BWI76_RS14855 0.55 -0.6
3,112,435 + BWI76_RS14855 0.77 -0.2
3,112,527 + BWI76_RS14855 0.86 -0.2
3,112,554 + BWI76_RS14855 0.89 -1.4
3,112,712 - -0.1
3,112,743 + -0.7
3,112,859 + -0.3
3,112,859 + +0.4
3,112,962 - BWI76_RS14860 0.16 -1.4
3,113,005 - BWI76_RS14860 0.18 +0.7
3,113,052 - BWI76_RS14860 0.22 +1.6
3,113,297 - BWI76_RS14860 0.38 -0.8
3,113,344 - BWI76_RS14860 0.41 -0.0
3,113,551 + BWI76_RS14860 0.54 +0.5
3,113,602 - BWI76_RS14860 0.57 -0.6

Or see this region's nucleotide sequence