Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03370 and BWI76_RS03375 are separated by 114 nucleotides BWI76_RS03375 and BWI76_RS03380 are separated by 179 nucleotides BWI76_RS03380 and BWI76_RS03385 are separated by 186 nucleotides BWI76_RS03385 and BWI76_RS03390 are separated by 113 nucleotides
BWI76_RS03370: BWI76_RS03370 - type I restriction-modification system subunit S, at 694,751 to 696,202
_RS03370
BWI76_RS03375: BWI76_RS03375 - transcriptional regulator, at 696,317 to 696,595
_RS03375
BWI76_RS03380: BWI76_RS03380 - hypothetical protein, at 696,775 to 697,692
_RS03380
BWI76_RS03385: BWI76_RS03385 - hypothetical protein, at 697,879 to 698,430
_RS03385
BWI76_RS03390: BWI76_RS03390 - hypothetical protein, at 698,544 to 699,287
_RS03390
Position (kb) 696
697
698 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 695.918 kb on + strand, within BWI76_RS03370 at 696.084 kb on + strand at 696.084 kb on + strand at 696.084 kb on + strand at 696.084 kb on + strand at 696.091 kb on + strand at 696.092 kb on - strand at 696.096 kb on + strand at 696.098 kb on + strand at 696.098 kb on + strand at 696.098 kb on + strand at 696.098 kb on + strand at 696.098 kb on + strand at 696.106 kb on - strand at 696.127 kb on + strand at 696.195 kb on + strand at 696.372 kb on - strand, within BWI76_RS03375 at 696.372 kb on - strand, within BWI76_RS03375 at 696.372 kb on - strand, within BWI76_RS03375 at 696.372 kb on - strand, within BWI76_RS03375 at 696.372 kb on - strand, within BWI76_RS03375 at 696.372 kb on - strand, within BWI76_RS03375 at 696.422 kb on + strand, within BWI76_RS03375 at 696.422 kb on + strand, within BWI76_RS03375 at 696.422 kb on + strand at 696.423 kb on + strand, within BWI76_RS03375 at 696.423 kb on + strand, within BWI76_RS03375 at 696.423 kb on + strand, within BWI76_RS03375 at 696.423 kb on + strand, within BWI76_RS03375 at 696.430 kb on - strand, within BWI76_RS03375 at 696.430 kb on - strand, within BWI76_RS03375 at 696.430 kb on - strand, within BWI76_RS03375 at 696.430 kb on - strand, within BWI76_RS03375 at 696.430 kb on - strand, within BWI76_RS03375 at 696.430 kb on - strand, within BWI76_RS03375 at 696.431 kb on - strand, within BWI76_RS03375 at 696.431 kb on - strand, within BWI76_RS03375 at 696.431 kb on - strand, within BWI76_RS03375 at 696.431 kb on - strand, within BWI76_RS03375 at 696.431 kb on - strand, within BWI76_RS03375 at 696.431 kb on - strand, within BWI76_RS03375 at 696.448 kb on - strand, within BWI76_RS03375 at 696.456 kb on + strand, within BWI76_RS03375 at 696.458 kb on - strand, within BWI76_RS03375 at 696.492 kb on + strand, within BWI76_RS03375 at 696.492 kb on + strand, within BWI76_RS03375 at 696.492 kb on + strand, within BWI76_RS03375 at 696.493 kb on + strand, within BWI76_RS03375 at 696.493 kb on + strand, within BWI76_RS03375 at 696.500 kb on - strand, within BWI76_RS03375 at 696.500 kb on - strand, within BWI76_RS03375 at 696.500 kb on - strand, within BWI76_RS03375 at 696.500 kb on - strand, within BWI76_RS03375 at 696.500 kb on - strand, within BWI76_RS03375 at 696.501 kb on - strand, within BWI76_RS03375 at 696.511 kb on - strand, within BWI76_RS03375 at 696.514 kb on + strand, within BWI76_RS03375 at 696.514 kb on + strand, within BWI76_RS03375 at 696.514 kb on + strand, within BWI76_RS03375 at 696.514 kb on + strand, within BWI76_RS03375 at 696.522 kb on - strand, within BWI76_RS03375 at 696.522 kb on - strand, within BWI76_RS03375 at 696.522 kb on - strand, within BWI76_RS03375 at 696.522 kb on - strand, within BWI76_RS03375 at 696.522 kb on - strand, within BWI76_RS03375 at 696.522 kb on - strand, within BWI76_RS03375 at 696.522 kb on - strand, within BWI76_RS03375 at 696.527 kb on - strand, within BWI76_RS03375 at 696.545 kb on - strand, within BWI76_RS03375 at 696.573 kb on + strand at 696.581 kb on - strand at 696.600 kb on - strand at 696.615 kb on + strand at 696.678 kb on - strand at 696.682 kb on + strand at 696.690 kb on - strand at 696.720 kb on + strand at 696.720 kb on + strand at 696.720 kb on + strand at 696.741 kb on + strand at 696.741 kb on + strand at 696.769 kb on + strand at 696.771 kb on + strand at 696.811 kb on + strand at 696.832 kb on - strand at 696.887 kb on + strand, within BWI76_RS03380 at 696.887 kb on + strand, within BWI76_RS03380 at 696.900 kb on + strand, within BWI76_RS03380 at 696.900 kb on + strand, within BWI76_RS03380 at 696.900 kb on + strand, within BWI76_RS03380 at 696.900 kb on + strand, within BWI76_RS03380 at 696.900 kb on + strand, within BWI76_RS03380 at 696.908 kb on - strand, within BWI76_RS03380 at 696.908 kb on - strand, within BWI76_RS03380 at 696.908 kb on - strand, within BWI76_RS03380 at 696.908 kb on - strand, within BWI76_RS03380 at 696.921 kb on - strand, within BWI76_RS03380 at 696.929 kb on + strand, within BWI76_RS03380 at 696.931 kb on + strand, within BWI76_RS03380 at 696.938 kb on + strand, within BWI76_RS03380 at 696.938 kb on - strand, within BWI76_RS03380 at 696.939 kb on - strand, within BWI76_RS03380 at 697.012 kb on + strand, within BWI76_RS03380 at 697.020 kb on - strand, within BWI76_RS03380 at 697.020 kb on - strand, within BWI76_RS03380 at 697.039 kb on - strand, within BWI76_RS03380 at 697.052 kb on - strand, within BWI76_RS03380 at 697.088 kb on - strand, within BWI76_RS03380 at 697.095 kb on - strand, within BWI76_RS03380 at 697.254 kb on + strand, within BWI76_RS03380 at 697.531 kb on + strand, within BWI76_RS03380 at 697.531 kb on + strand, within BWI76_RS03380 at 697.531 kb on + strand, within BWI76_RS03380 at 697.539 kb on - strand, within BWI76_RS03380 at 697.539 kb on - strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.541 kb on + strand, within BWI76_RS03380 at 697.542 kb on + strand, within BWI76_RS03380 at 697.549 kb on + strand, within BWI76_RS03380 at 697.549 kb on - strand, within BWI76_RS03380 at 697.624 kb on + strand at 697.627 kb on + strand at 697.632 kb on + strand at 697.639 kb on + strand at 697.639 kb on + strand at 697.639 kb on + strand at 697.639 kb on + strand at 697.639 kb on + strand at 697.639 kb on + strand at 697.639 kb on + strand at 697.639 kb on + strand at 697.647 kb on - strand at 697.647 kb on - strand at 697.647 kb on - strand at 697.704 kb on + strand at 697.754 kb on - strand at 697.835 kb on + strand at 697.835 kb on + strand at 697.860 kb on + strand at 697.860 kb on + strand at 697.860 kb on + strand at 697.868 kb on - strand at 697.961 kb on + strand, within BWI76_RS03385 at 697.969 kb on - strand, within BWI76_RS03385 at 697.994 kb on + strand, within BWI76_RS03385 at 698.002 kb on + strand, within BWI76_RS03385 at 698.027 kb on + strand, within BWI76_RS03385 at 698.036 kb on - strand, within BWI76_RS03385 at 698.039 kb on + strand, within BWI76_RS03385 at 698.127 kb on + strand, within BWI76_RS03385 at 698.163 kb on + strand, within BWI76_RS03385 at 698.163 kb on + strand, within BWI76_RS03385 at 698.163 kb on + strand at 698.171 kb on + strand, within BWI76_RS03385 at 698.171 kb on + strand, within BWI76_RS03385 at 698.171 kb on + strand, within BWI76_RS03385 at 698.171 kb on + strand, within BWI76_RS03385 at 698.171 kb on - strand, within BWI76_RS03385 at 698.232 kb on + strand, within BWI76_RS03385 at 698.232 kb on + strand, within BWI76_RS03385 at 698.233 kb on + strand, within BWI76_RS03385 at 698.240 kb on + strand, within BWI76_RS03385 at 698.246 kb on + strand, within BWI76_RS03385 at 698.247 kb on + strand, within BWI76_RS03385 at 698.247 kb on + strand, within BWI76_RS03385 at 698.247 kb on - strand, within BWI76_RS03385 at 698.255 kb on - strand, within BWI76_RS03385 at 698.259 kb on + strand, within BWI76_RS03385 at 698.259 kb on + strand at 698.259 kb on + strand, within BWI76_RS03385 at 698.259 kb on + strand, within BWI76_RS03385 at 698.259 kb on + strand, within BWI76_RS03385 at 698.267 kb on - strand, within BWI76_RS03385 at 698.267 kb on - strand, within BWI76_RS03385 at 698.268 kb on - strand, within BWI76_RS03385 at 698.268 kb on - strand, within BWI76_RS03385 at 698.289 kb on + strand, within BWI76_RS03385 at 698.313 kb on + strand, within BWI76_RS03385 at 698.366 kb on + strand, within BWI76_RS03385 at 698.366 kb on + strand, within BWI76_RS03385 at 698.366 kb on + strand, within BWI76_RS03385 at 698.380 kb on + strand at 698.388 kb on - strand at 698.388 kb on - strand at 698.472 kb on + strand at 698.479 kb on + strand at 698.479 kb on + strand at 698.479 kb on + strand at 698.479 kb on + strand at 698.479 kb on + strand at 698.479 kb on + strand at 698.479 kb on + strand at 698.479 kb on + strand at 698.487 kb on - strand at 698.487 kb on - strand at 698.487 kb on - strand at 698.500 kb on + strand at 698.505 kb on + strand at 698.508 kb on - strand at 698.555 kb on + strand at 698.573 kb on + strand at 698.573 kb on + strand at 698.582 kb on + strand at 698.590 kb on - strand at 698.591 kb on + strand at 698.592 kb on + strand at 698.592 kb on + strand at 698.592 kb on + strand at 698.592 kb on + strand at 698.592 kb on + strand at 698.592 kb on + strand at 698.592 kb on + strand at 698.592 kb on + strand at 698.592 kb on + strand at 698.595 kb on + strand at 698.597 kb on + strand at 698.597 kb on + strand at 698.600 kb on - strand at 698.600 kb on - strand at 698.600 kb on - strand at 698.600 kb on - strand at 698.600 kb on - strand at 698.600 kb on - strand at 698.600 kb on - strand at 698.600 kb on - strand at 698.602 kb on + strand at 698.606 kb on + strand at 698.609 kb on + strand at 698.614 kb on + strand at 698.614 kb on - strand at 698.616 kb on + strand at 698.632 kb on + strand, within BWI76_RS03390 at 698.640 kb on - strand, within BWI76_RS03390 at 698.644 kb on + strand, within BWI76_RS03390 at 698.644 kb on + strand, within BWI76_RS03390 at 698.644 kb on + strand, within BWI76_RS03390 at 698.644 kb on + strand, within BWI76_RS03390 at 698.646 kb on - strand, within BWI76_RS03390 at 698.652 kb on - strand, within BWI76_RS03390 at 698.652 kb on - strand, within BWI76_RS03390 at 698.652 kb on - strand, within BWI76_RS03390 at 698.654 kb on + strand, within BWI76_RS03390 at 698.662 kb on - strand, within BWI76_RS03390 at 698.663 kb on + strand, within BWI76_RS03390 at 698.683 kb on + strand at 698.691 kb on - strand, within BWI76_RS03390
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM and Betaine 100 uM remove 695,918 + BWI76_RS03370 0.80 +0.4 696,084 + -0.1 696,084 + +0.8 696,084 + +1.9 696,084 + -1.0 696,091 + +0.6 696,092 - -1.9 696,096 + +0.6 696,098 + +0.6 696,098 + +1.0 696,098 + +0.0 696,098 + +0.6 696,098 + +0.5 696,106 - +2.0 696,127 + -0.0 696,195 + -2.3 696,372 - BWI76_RS03375 0.20 -2.3 696,372 - BWI76_RS03375 0.20 -1.7 696,372 - BWI76_RS03375 0.20 +2.0 696,372 - BWI76_RS03375 0.20 +0.8 696,372 - BWI76_RS03375 0.20 -0.7 696,372 - BWI76_RS03375 0.20 +4.1 696,422 + BWI76_RS03375 0.38 +1.6 696,422 + BWI76_RS03375 0.38 +1.6 696,422 + +0.6 696,423 + BWI76_RS03375 0.38 -0.7 696,423 + BWI76_RS03375 0.38 -1.6 696,423 + BWI76_RS03375 0.38 +0.0 696,423 + BWI76_RS03375 0.38 -0.1 696,430 - BWI76_RS03375 0.41 +0.5 696,430 - BWI76_RS03375 0.41 +0.6 696,430 - BWI76_RS03375 0.41 +0.4 696,430 - BWI76_RS03375 0.41 -0.4 696,430 - BWI76_RS03375 0.41 +0.6 696,430 - BWI76_RS03375 0.41 -1.6 696,431 - BWI76_RS03375 0.41 -0.4 696,431 - BWI76_RS03375 0.41 -0.4 696,431 - BWI76_RS03375 0.41 -1.4 696,431 - BWI76_RS03375 0.41 -0.7 696,431 - BWI76_RS03375 0.41 +0.6 696,431 - BWI76_RS03375 0.41 -1.9 696,448 - BWI76_RS03375 0.47 -1.3 696,456 + BWI76_RS03375 0.50 +1.6 696,458 - BWI76_RS03375 0.51 +0.1 696,492 + BWI76_RS03375 0.63 -1.4 696,492 + BWI76_RS03375 0.63 -0.7 696,492 + BWI76_RS03375 0.63 -2.0 696,493 + BWI76_RS03375 0.63 -0.6 696,493 + BWI76_RS03375 0.63 +1.6 696,500 - BWI76_RS03375 0.66 +0.0 696,500 - BWI76_RS03375 0.66 -0.4 696,500 - BWI76_RS03375 0.66 -0.4 696,500 - BWI76_RS03375 0.66 -0.9 696,500 - BWI76_RS03375 0.66 -2.3 696,501 - BWI76_RS03375 0.66 -0.4 696,511 - BWI76_RS03375 0.70 +1.6 696,514 + BWI76_RS03375 0.71 +0.2 696,514 + BWI76_RS03375 0.71 +0.6 696,514 + BWI76_RS03375 0.71 -1.9 696,514 + BWI76_RS03375 0.71 +2.6 696,522 - BWI76_RS03375 0.73 -0.3 696,522 - BWI76_RS03375 0.73 +0.2 696,522 - BWI76_RS03375 0.73 +0.6 696,522 - BWI76_RS03375 0.73 +0.5 696,522 - BWI76_RS03375 0.73 -1.2 696,522 - BWI76_RS03375 0.73 -0.6 696,522 - BWI76_RS03375 0.73 -0.9 696,527 - BWI76_RS03375 0.75 -2.1 696,545 - BWI76_RS03375 0.82 +0.5 696,573 + +1.6 696,581 - -0.8 696,600 - +0.4 696,615 + +1.3 696,678 - +1.6 696,682 + -0.4 696,690 - +2.8 696,720 + -0.1 696,720 + +0.8 696,720 + +0.4 696,741 + -0.1 696,741 + -0.2 696,769 + +0.2 696,771 + +0.4 696,811 + -0.5 696,832 - +1.6 696,887 + BWI76_RS03380 0.12 +1.6 696,887 + BWI76_RS03380 0.12 +0.4 696,900 + BWI76_RS03380 0.14 -1.0 696,900 + BWI76_RS03380 0.14 -1.0 696,900 + BWI76_RS03380 0.14 -0.9 696,900 + BWI76_RS03380 0.14 +0.5 696,900 + BWI76_RS03380 0.14 -1.0 696,908 - BWI76_RS03380 0.14 -0.2 696,908 - BWI76_RS03380 0.14 +1.0 696,908 - BWI76_RS03380 0.14 +0.6 696,908 - BWI76_RS03380 0.14 +3.2 696,921 - BWI76_RS03380 0.16 +1.6 696,929 + BWI76_RS03380 0.17 -2.6 696,931 + BWI76_RS03380 0.17 -0.7 696,938 + BWI76_RS03380 0.18 -2.6 696,938 - BWI76_RS03380 0.18 -0.1 696,939 - BWI76_RS03380 0.18 +0.2 697,012 + BWI76_RS03380 0.26 +0.0 697,020 - BWI76_RS03380 0.27 -1.0 697,020 - BWI76_RS03380 0.27 +1.6 697,039 - BWI76_RS03380 0.29 +0.0 697,052 - BWI76_RS03380 0.30 -1.0 697,088 - BWI76_RS03380 0.34 +0.3 697,095 - BWI76_RS03380 0.35 +0.0 697,254 + BWI76_RS03380 0.52 -1.6 697,531 + BWI76_RS03380 0.82 -2.0 697,531 + BWI76_RS03380 0.82 -1.4 697,531 + BWI76_RS03380 0.82 +1.6 697,539 - BWI76_RS03380 0.83 -0.6 697,539 - BWI76_RS03380 0.83 -0.4 697,541 + BWI76_RS03380 0.83 -0.3 697,541 + BWI76_RS03380 0.83 +1.6 697,541 + BWI76_RS03380 0.83 +1.6 697,541 + BWI76_RS03380 0.83 +1.6 697,541 + BWI76_RS03380 0.83 +0.5 697,541 + BWI76_RS03380 0.83 -1.5 697,541 + BWI76_RS03380 0.83 -1.2 697,541 + BWI76_RS03380 0.83 +0.0 697,541 + BWI76_RS03380 0.83 -0.4 697,541 + BWI76_RS03380 0.83 -1.0 697,541 + BWI76_RS03380 0.83 -1.4 697,541 + BWI76_RS03380 0.83 -1.2 697,542 + BWI76_RS03380 0.84 -1.4 697,549 + BWI76_RS03380 0.84 -0.1 697,549 - BWI76_RS03380 0.84 -1.6 697,624 + -0.7 697,627 + -0.4 697,632 + -0.7 697,639 + -1.4 697,639 + +0.1 697,639 + +0.0 697,639 + -1.4 697,639 + -2.0 697,639 + +0.0 697,639 + +0.6 697,639 + -0.6 697,647 - +1.6 697,647 - +0.0 697,647 - +3.9 697,704 + -1.9 697,754 - +0.6 697,835 + +1.6 697,835 + +0.0 697,860 + +3.2 697,860 + -2.4 697,860 + +0.0 697,868 - -1.3 697,961 + BWI76_RS03385 0.15 +0.7 697,969 - BWI76_RS03385 0.16 -0.6 697,994 + BWI76_RS03385 0.21 +1.6 698,002 + BWI76_RS03385 0.22 -1.2 698,027 + BWI76_RS03385 0.27 -2.4 698,036 - BWI76_RS03385 0.28 +1.0 698,039 + BWI76_RS03385 0.29 +1.6 698,127 + BWI76_RS03385 0.45 +1.6 698,163 + BWI76_RS03385 0.51 -0.8 698,163 + BWI76_RS03385 0.51 +0.6 698,163 + +0.6 698,171 + BWI76_RS03385 0.53 -1.4 698,171 + BWI76_RS03385 0.53 -2.2 698,171 + BWI76_RS03385 0.53 +1.6 698,171 + BWI76_RS03385 0.53 -0.6 698,171 - BWI76_RS03385 0.53 -1.0 698,232 + BWI76_RS03385 0.64 -0.5 698,232 + BWI76_RS03385 0.64 -2.1 698,233 + BWI76_RS03385 0.64 +1.6 698,240 + BWI76_RS03385 0.65 -0.4 698,246 + BWI76_RS03385 0.66 +1.9 698,247 + BWI76_RS03385 0.67 +1.3 698,247 + BWI76_RS03385 0.67 -1.4 698,247 - BWI76_RS03385 0.67 -0.8 698,255 - BWI76_RS03385 0.68 -0.7 698,259 + BWI76_RS03385 0.69 +1.6 698,259 + +1.6 698,259 + BWI76_RS03385 0.69 -0.6 698,259 + BWI76_RS03385 0.69 -0.8 698,259 + BWI76_RS03385 0.69 +1.3 698,267 - BWI76_RS03385 0.70 -2.3 698,267 - BWI76_RS03385 0.70 +1.6 698,268 - BWI76_RS03385 0.70 +0.2 698,268 - BWI76_RS03385 0.70 +1.6 698,289 + BWI76_RS03385 0.74 +0.2 698,313 + BWI76_RS03385 0.79 -1.0 698,366 + BWI76_RS03385 0.88 -1.7 698,366 + BWI76_RS03385 0.88 -1.1 698,366 + BWI76_RS03385 0.88 +0.5 698,380 + +2.2 698,388 - +0.0 698,388 - +0.1 698,472 + -0.5 698,479 + -1.0 698,479 + -0.1 698,479 + +0.2 698,479 + +0.4 698,479 + -3.5 698,479 + -1.9 698,479 + -1.7 698,479 + -0.4 698,487 - +0.2 698,487 - +1.0 698,487 - -0.1 698,500 + -0.1 698,505 + +0.6 698,508 - -1.0 698,555 + -1.3 698,573 + +0.2 698,573 + +0.0 698,582 + -0.2 698,590 - +0.2 698,591 + +0.4 698,592 + +0.3 698,592 + -0.1 698,592 + -0.1 698,592 + -0.7 698,592 + -1.8 698,592 + -3.2 698,592 + +0.0 698,592 + -0.3 698,592 + +1.6 698,595 + +0.0 698,597 + -0.9 698,597 + +0.5 698,600 - +3.6 698,600 - -0.7 698,600 - -0.1 698,600 - +0.0 698,600 - +0.5 698,600 - -2.6 698,600 - +0.6 698,600 - +1.0 698,602 + -0.7 698,606 + -1.0 698,609 + -0.4 698,614 + +1.6 698,614 - +0.6 698,616 + +0.0 698,632 + BWI76_RS03390 0.12 -1.5 698,640 - BWI76_RS03390 0.13 -0.1 698,644 + BWI76_RS03390 0.13 -1.8 698,644 + BWI76_RS03390 0.13 +0.8 698,644 + BWI76_RS03390 0.13 -0.6 698,644 + BWI76_RS03390 0.13 +0.6 698,646 - BWI76_RS03390 0.14 +0.1 698,652 - BWI76_RS03390 0.15 -1.6 698,652 - BWI76_RS03390 0.15 -0.1 698,652 - BWI76_RS03390 0.15 -0.1 698,654 + BWI76_RS03390 0.15 +0.7 698,662 - BWI76_RS03390 0.16 +1.3 698,663 + BWI76_RS03390 0.16 +0.3 698,683 + -1.0 698,691 - BWI76_RS03390 0.20 -0.2
Or see this region's nucleotide sequence