Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13340 and BWI76_RS13345 are separated by 138 nucleotides BWI76_RS13345 and BWI76_RS13350 are separated by 88 nucleotides
BWI76_RS13340: BWI76_RS13340 - peptide ABC transporter substrate-binding protein, at 2,781,998 to 2,783,590
_RS13340
BWI76_RS13345: BWI76_RS13345 - DUF3313 domain-containing protein, at 2,783,729 to 2,784,397
_RS13345
BWI76_RS13350: BWI76_RS13350 - EamA family transporter, at 2,784,486 to 2,785,394
_RS13350
Position (kb)
2783
2784
2785 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2782.731 kb on + strand, within BWI76_RS13340 at 2782.819 kb on - strand, within BWI76_RS13340 at 2782.839 kb on - strand, within BWI76_RS13340 at 2782.899 kb on + strand, within BWI76_RS13340 at 2782.899 kb on + strand, within BWI76_RS13340 at 2782.899 kb on + strand, within BWI76_RS13340 at 2782.907 kb on - strand, within BWI76_RS13340 at 2782.907 kb on - strand, within BWI76_RS13340 at 2782.907 kb on - strand, within BWI76_RS13340 at 2783.003 kb on + strand, within BWI76_RS13340 at 2783.064 kb on - strand, within BWI76_RS13340 at 2783.160 kb on - strand, within BWI76_RS13340 at 2783.160 kb on - strand, within BWI76_RS13340 at 2783.222 kb on - strand, within BWI76_RS13340 at 2783.222 kb on - strand, within BWI76_RS13340 at 2783.249 kb on - strand, within BWI76_RS13340 at 2783.272 kb on + strand, within BWI76_RS13340 at 2783.284 kb on - strand, within BWI76_RS13340 at 2783.349 kb on + strand, within BWI76_RS13340 at 2783.349 kb on + strand, within BWI76_RS13340 at 2783.349 kb on + strand, within BWI76_RS13340 at 2783.437 kb on + strand at 2783.442 kb on + strand at 2783.450 kb on - strand at 2783.450 kb on - strand at 2783.458 kb on + strand at 2783.462 kb on + strand at 2783.462 kb on + strand at 2783.463 kb on + strand at 2783.470 kb on - strand at 2783.473 kb on + strand at 2783.473 kb on + strand at 2783.473 kb on + strand at 2783.473 kb on + strand at 2783.481 kb on - strand at 2783.481 kb on - strand at 2783.481 kb on - strand at 2783.527 kb on + strand at 2783.527 kb on + strand at 2783.528 kb on - strand at 2783.675 kb on - strand at 2783.686 kb on + strand at 2783.717 kb on + strand at 2783.723 kb on + strand at 2783.725 kb on - strand at 2783.877 kb on + strand, within BWI76_RS13345 at 2783.877 kb on + strand, within BWI76_RS13345 at 2783.877 kb on + strand, within BWI76_RS13345 at 2783.885 kb on - strand, within BWI76_RS13345 at 2783.887 kb on + strand, within BWI76_RS13345 at 2783.949 kb on + strand, within BWI76_RS13345 at 2783.949 kb on + strand, within BWI76_RS13345 at 2783.957 kb on - strand, within BWI76_RS13345 at 2783.973 kb on + strand, within BWI76_RS13345 at 2783.974 kb on - strand, within BWI76_RS13345 at 2784.018 kb on + strand, within BWI76_RS13345 at 2784.082 kb on - strand, within BWI76_RS13345 at 2784.298 kb on - strand, within BWI76_RS13345 at 2784.569 kb on + strand at 2784.655 kb on + strand, within BWI76_RS13350 at 2784.696 kb on + strand, within BWI76_RS13350 at 2784.744 kb on + strand, within BWI76_RS13350 at 2784.904 kb on - strand, within BWI76_RS13350 at 2784.978 kb on + strand, within BWI76_RS13350 at 2785.265 kb on + strand, within BWI76_RS13350 at 2785.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM remove 2,782,731 + BWI76_RS13340 0.46 +0.5 2,782,819 - BWI76_RS13340 0.52 -0.2 2,782,839 - BWI76_RS13340 0.53 +1.9 2,782,899 + BWI76_RS13340 0.57 +0.1 2,782,899 + BWI76_RS13340 0.57 -1.4 2,782,899 + BWI76_RS13340 0.57 +0.6 2,782,907 - BWI76_RS13340 0.57 -0.5 2,782,907 - BWI76_RS13340 0.57 -1.4 2,782,907 - BWI76_RS13340 0.57 +0.9 2,783,003 + BWI76_RS13340 0.63 -0.1 2,783,064 - BWI76_RS13340 0.67 -0.7 2,783,160 - BWI76_RS13340 0.73 +0.1 2,783,160 - BWI76_RS13340 0.73 -1.9 2,783,222 - BWI76_RS13340 0.77 -0.2 2,783,222 - BWI76_RS13340 0.77 -0.6 2,783,249 - BWI76_RS13340 0.79 -0.9 2,783,272 + BWI76_RS13340 0.80 -0.5 2,783,284 - BWI76_RS13340 0.81 +1.0 2,783,349 + BWI76_RS13340 0.85 -1.4 2,783,349 + BWI76_RS13340 0.85 -2.1 2,783,349 + BWI76_RS13340 0.85 +0.9 2,783,437 + -3.8 2,783,442 + -0.1 2,783,450 - -0.1 2,783,450 - +0.7 2,783,458 + -0.4 2,783,462 + +0.2 2,783,462 + -0.4 2,783,463 + +0.6 2,783,470 - -0.7 2,783,473 + +0.0 2,783,473 + +0.3 2,783,473 + -0.7 2,783,473 + +1.3 2,783,481 - -0.4 2,783,481 - +0.5 2,783,481 - -0.7 2,783,527 + +0.6 2,783,527 + +0.4 2,783,528 - +0.4 2,783,675 - +0.4 2,783,686 + -0.1 2,783,717 + -1.0 2,783,723 + -1.7 2,783,725 - -0.5 2,783,877 + BWI76_RS13345 0.22 +0.1 2,783,877 + BWI76_RS13345 0.22 -1.9 2,783,877 + BWI76_RS13345 0.22 +0.1 2,783,885 - BWI76_RS13345 0.23 -0.9 2,783,887 + BWI76_RS13345 0.24 -0.4 2,783,949 + BWI76_RS13345 0.33 -0.6 2,783,949 + BWI76_RS13345 0.33 -1.1 2,783,957 - BWI76_RS13345 0.34 -0.7 2,783,973 + BWI76_RS13345 0.36 -0.9 2,783,974 - BWI76_RS13345 0.37 -0.2 2,784,018 + BWI76_RS13345 0.43 -2.0 2,784,082 - BWI76_RS13345 0.53 -1.8 2,784,298 - BWI76_RS13345 0.85 -1.4 2,784,569 + +0.2 2,784,655 + BWI76_RS13350 0.19 +0.1 2,784,696 + BWI76_RS13350 0.23 +1.1 2,784,744 + BWI76_RS13350 0.28 -1.0 2,784,904 - BWI76_RS13350 0.46 -0.9 2,784,978 + BWI76_RS13350 0.54 -0.1 2,785,265 + BWI76_RS13350 0.86 -0.6 2,785,383 - -0.6
Or see this region's nucleotide sequence