Experiment: RCH2_defined_1percent_glucose with Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05585 and BWI76_RS05590 overlap by 8 nucleotides BWI76_RS05590 and BWI76_RS05595 overlap by 10 nucleotides
BWI76_RS05580: BWI76_RS05580 - group 1 glycosyl transferase, at 1,168,111 to 1,169,325
_RS05580
BWI76_RS05585: BWI76_RS05585 - family 2 glycosyl transferase, at 1,169,306 to 1,170,331
_RS05585
BWI76_RS05590: BWI76_RS05590 - hypothetical protein, at 1,170,324 to 1,171,706
_RS05590
BWI76_RS05595: BWI76_RS05595 - capsular polysaccharide biosynthesis protein, at 1,171,697 to 1,172,914
_RS05595
Position (kb)
1170
1171
1172 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1169.355 kb on - strand at 1169.356 kb on - strand at 1169.364 kb on - strand at 1169.374 kb on - strand at 1169.393 kb on + strand at 1169.395 kb on + strand at 1169.399 kb on - strand at 1169.401 kb on - strand at 1169.401 kb on - strand at 1169.401 kb on - strand at 1169.410 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.447 kb on + strand, within BWI76_RS05585 at 1169.454 kb on - strand, within BWI76_RS05585 at 1169.455 kb on - strand, within BWI76_RS05585 at 1169.455 kb on - strand, within BWI76_RS05585 at 1169.490 kb on - strand, within BWI76_RS05585 at 1169.505 kb on + strand, within BWI76_RS05585 at 1169.568 kb on + strand, within BWI76_RS05585 at 1169.593 kb on - strand, within BWI76_RS05585 at 1169.632 kb on + strand, within BWI76_RS05585 at 1169.653 kb on + strand, within BWI76_RS05585 at 1169.713 kb on + strand, within BWI76_RS05585 at 1169.726 kb on - strand, within BWI76_RS05585 at 1169.800 kb on - strand, within BWI76_RS05585 at 1169.803 kb on + strand, within BWI76_RS05585 at 1169.811 kb on - strand, within BWI76_RS05585 at 1169.826 kb on - strand, within BWI76_RS05585 at 1169.826 kb on - strand, within BWI76_RS05585 at 1169.826 kb on - strand, within BWI76_RS05585 at 1169.861 kb on + strand, within BWI76_RS05585 at 1169.892 kb on + strand, within BWI76_RS05585 at 1169.900 kb on - strand, within BWI76_RS05585 at 1169.963 kb on - strand, within BWI76_RS05585 at 1170.022 kb on - strand, within BWI76_RS05585 at 1170.109 kb on + strand, within BWI76_RS05585 at 1170.228 kb on + strand, within BWI76_RS05585 at 1170.228 kb on + strand, within BWI76_RS05585 at 1170.236 kb on - strand at 1170.275 kb on + strand at 1170.438 kb on + strand at 1170.440 kb on + strand at 1170.448 kb on - strand at 1170.448 kb on - strand at 1170.490 kb on + strand, within BWI76_RS05590 at 1170.490 kb on + strand, within BWI76_RS05590 at 1170.490 kb on + strand, within BWI76_RS05590 at 1170.498 kb on - strand, within BWI76_RS05590 at 1170.516 kb on + strand, within BWI76_RS05590 at 1170.524 kb on - strand, within BWI76_RS05590 at 1170.545 kb on - strand, within BWI76_RS05590 at 1170.571 kb on - strand, within BWI76_RS05590 at 1170.571 kb on - strand, within BWI76_RS05590 at 1170.571 kb on - strand, within BWI76_RS05590 at 1170.653 kb on + strand, within BWI76_RS05590 at 1170.669 kb on + strand, within BWI76_RS05590 at 1170.715 kb on - strand, within BWI76_RS05590 at 1170.996 kb on + strand, within BWI76_RS05590 at 1171.020 kb on - strand, within BWI76_RS05590 at 1171.020 kb on - strand, within BWI76_RS05590 at 1171.165 kb on - strand, within BWI76_RS05590 at 1171.735 kb on - strand at 1171.786 kb on + strand at 1171.822 kb on + strand, within BWI76_RS05595 at 1171.825 kb on - strand, within BWI76_RS05595 at 1171.827 kb on + strand, within BWI76_RS05595 at 1171.835 kb on - strand, within BWI76_RS05595 at 1172.055 kb on + strand, within BWI76_RS05595 at 1172.370 kb on - strand, within BWI76_RS05595 at 1172.451 kb on + strand, within BWI76_RS05595 at 1172.451 kb on + strand, within BWI76_RS05595 at 1172.451 kb on + strand, within BWI76_RS05595 at 1172.451 kb on + strand, within BWI76_RS05595 at 1172.459 kb on - strand, within BWI76_RS05595 at 1172.485 kb on + strand, within BWI76_RS05595
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Betaine 100 uM remove 1,169,355 - -0.5 1,169,356 - -0.3 1,169,364 - -0.1 1,169,374 - +0.6 1,169,393 + -1.2 1,169,395 + -0.1 1,169,399 - -0.2 1,169,401 - +0.2 1,169,401 - -0.3 1,169,401 - +1.1 1,169,410 - BWI76_RS05585 0.10 +0.1 1,169,416 - BWI76_RS05585 0.11 +0.5 1,169,416 - BWI76_RS05585 0.11 -0.0 1,169,416 - BWI76_RS05585 0.11 +1.8 1,169,416 - BWI76_RS05585 0.11 -0.5 1,169,416 - BWI76_RS05585 0.11 -0.0 1,169,447 + BWI76_RS05585 0.14 -2.0 1,169,454 - BWI76_RS05585 0.14 +0.4 1,169,455 - BWI76_RS05585 0.15 +0.9 1,169,455 - BWI76_RS05585 0.15 -0.5 1,169,490 - BWI76_RS05585 0.18 -1.5 1,169,505 + BWI76_RS05585 0.19 +1.7 1,169,568 + BWI76_RS05585 0.26 +0.3 1,169,593 - BWI76_RS05585 0.28 +2.8 1,169,632 + BWI76_RS05585 0.32 -0.0 1,169,653 + BWI76_RS05585 0.34 -0.2 1,169,713 + BWI76_RS05585 0.40 +0.1 1,169,726 - BWI76_RS05585 0.41 +0.2 1,169,800 - BWI76_RS05585 0.48 +0.1 1,169,803 + BWI76_RS05585 0.48 +0.8 1,169,811 - BWI76_RS05585 0.49 +0.2 1,169,826 - BWI76_RS05585 0.51 -1.3 1,169,826 - BWI76_RS05585 0.51 -0.1 1,169,826 - BWI76_RS05585 0.51 +0.4 1,169,861 + BWI76_RS05585 0.54 -0.6 1,169,892 + BWI76_RS05585 0.57 +0.8 1,169,900 - BWI76_RS05585 0.58 -0.4 1,169,963 - BWI76_RS05585 0.64 -0.2 1,170,022 - BWI76_RS05585 0.70 +1.1 1,170,109 + BWI76_RS05585 0.78 -0.0 1,170,228 + BWI76_RS05585 0.90 -0.9 1,170,228 + BWI76_RS05585 0.90 -2.6 1,170,236 - +0.4 1,170,275 + -0.6 1,170,438 + +0.4 1,170,440 + +1.0 1,170,448 - -0.3 1,170,448 - +2.4 1,170,490 + BWI76_RS05590 0.12 -1.5 1,170,490 + BWI76_RS05590 0.12 +0.4 1,170,490 + BWI76_RS05590 0.12 -0.3 1,170,498 - BWI76_RS05590 0.13 +0.7 1,170,516 + BWI76_RS05590 0.14 -0.9 1,170,524 - BWI76_RS05590 0.14 +0.3 1,170,545 - BWI76_RS05590 0.16 +1.8 1,170,571 - BWI76_RS05590 0.18 +0.3 1,170,571 - BWI76_RS05590 0.18 +0.5 1,170,571 - BWI76_RS05590 0.18 -2.3 1,170,653 + BWI76_RS05590 0.24 +1.0 1,170,669 + BWI76_RS05590 0.25 +0.0 1,170,715 - BWI76_RS05590 0.28 +0.8 1,170,996 + BWI76_RS05590 0.49 +0.9 1,171,020 - BWI76_RS05590 0.50 -0.2 1,171,020 - BWI76_RS05590 0.50 +0.6 1,171,165 - BWI76_RS05590 0.61 +0.8 1,171,735 - +0.1 1,171,786 + +0.3 1,171,822 + BWI76_RS05595 0.10 +1.4 1,171,825 - BWI76_RS05595 0.11 +0.3 1,171,827 + BWI76_RS05595 0.11 -0.3 1,171,835 - BWI76_RS05595 0.11 -0.1 1,172,055 + BWI76_RS05595 0.29 -1.0 1,172,370 - BWI76_RS05595 0.55 +0.4 1,172,451 + BWI76_RS05595 0.62 -0.9 1,172,451 + BWI76_RS05595 0.62 +0.1 1,172,451 + BWI76_RS05595 0.62 +0.5 1,172,451 + BWI76_RS05595 0.62 +0.2 1,172,459 - BWI76_RS05595 0.63 -0.4 1,172,485 + BWI76_RS05595 0.65 +0.3
Or see this region's nucleotide sequence