Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27250

Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27240 and BWI76_RS27245 overlap by 4 nucleotidesBWI76_RS27245 and BWI76_RS27250 are separated by 160 nucleotidesBWI76_RS27250 and BWI76_RS27255 are separated by 9 nucleotides BWI76_RS27240: BWI76_RS27240 - hypothetical protein, at 5,653,591 to 5,654,523 _RS27240 BWI76_RS27245: BWI76_RS27245 - glycosyl transferase family protein, at 5,654,520 to 5,655,317 _RS27245 BWI76_RS27250: BWI76_RS27250 - L-threonine 3-dehydrogenase, at 5,655,478 to 5,656,503 _RS27250 BWI76_RS27255: BWI76_RS27255 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data), at 5,656,513 to 5,657,706 _RS27255 Position (kb) 5655 5656 5657Strain fitness (log2 ratio) -2 -1 0 1 2at 5654.568 kb on + strandat 5654.571 kb on - strandat 5654.588 kb on - strandat 5654.621 kb on + strand, within BWI76_RS27245at 5654.644 kb on + strand, within BWI76_RS27245at 5654.673 kb on - strand, within BWI76_RS27245at 5654.799 kb on + strand, within BWI76_RS27245at 5654.805 kb on - strand, within BWI76_RS27245at 5654.807 kb on - strand, within BWI76_RS27245at 5655.008 kb on - strand, within BWI76_RS27245at 5655.024 kb on - strand, within BWI76_RS27245at 5655.038 kb on - strand, within BWI76_RS27245at 5655.065 kb on + strand, within BWI76_RS27245at 5655.075 kb on + strand, within BWI76_RS27245at 5655.094 kb on - strand, within BWI76_RS27245at 5655.094 kb on - strand, within BWI76_RS27245at 5655.142 kb on + strand, within BWI76_RS27245at 5655.258 kb on - strandat 5655.329 kb on + strandat 5655.330 kb on + strandat 5655.339 kb on + strandat 5655.347 kb on - strandat 5655.510 kb on + strandat 5655.525 kb on + strandat 5655.574 kb on - strandat 5655.605 kb on - strand, within BWI76_RS27250at 5655.798 kb on - strand, within BWI76_RS27250at 5655.798 kb on - strand, within BWI76_RS27250at 5655.815 kb on + strandat 5655.823 kb on - strand, within BWI76_RS27250at 5655.839 kb on + strand, within BWI76_RS27250at 5655.887 kb on - strand, within BWI76_RS27250at 5655.987 kb on - strand, within BWI76_RS27250at 5655.987 kb on - strand, within BWI76_RS27250at 5656.263 kb on + strand, within BWI76_RS27250at 5656.271 kb on - strand, within BWI76_RS27250at 5656.278 kb on + strand, within BWI76_RS27250at 5656.278 kb on + strand, within BWI76_RS27250at 5656.286 kb on - strand, within BWI76_RS27250at 5656.311 kb on + strand, within BWI76_RS27250at 5656.364 kb on - strand, within BWI76_RS27250at 5656.388 kb on - strand, within BWI76_RS27250at 5656.465 kb on + strandat 5656.510 kb on + strandat 5656.525 kb on + strandat 5656.545 kb on + strandat 5656.546 kb on + strandat 5656.560 kb on - strandat 5656.700 kb on + strand, within BWI76_RS27255at 5656.718 kb on - strand, within BWI76_RS27255at 5656.783 kb on + strand, within BWI76_RS27255at 5657.029 kb on - strand, within BWI76_RS27255at 5657.029 kb on - strand, within BWI76_RS27255at 5657.134 kb on - strand, within BWI76_RS27255at 5657.137 kb on + strand, within BWI76_RS27255at 5657.153 kb on + strand, within BWI76_RS27255at 5657.275 kb on - strand, within BWI76_RS27255at 5657.277 kb on + strand, within BWI76_RS27255at 5657.305 kb on + strand, within BWI76_RS27255at 5657.313 kb on - strand, within BWI76_RS27255at 5657.313 kb on - strand, within BWI76_RS27255at 5657.394 kb on - strand, within BWI76_RS27255at 5657.447 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM
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5,654,568 + +0.5
5,654,571 - +0.0
5,654,588 - +0.7
5,654,621 + BWI76_RS27245 0.13 -0.1
5,654,644 + BWI76_RS27245 0.16 -1.8
5,654,673 - BWI76_RS27245 0.19 +0.5
5,654,799 + BWI76_RS27245 0.35 +0.0
5,654,805 - BWI76_RS27245 0.36 +0.5
5,654,807 - BWI76_RS27245 0.36 -0.9
5,655,008 - BWI76_RS27245 0.61 +0.0
5,655,024 - BWI76_RS27245 0.63 -0.4
5,655,038 - BWI76_RS27245 0.65 +0.2
5,655,065 + BWI76_RS27245 0.68 +0.4
5,655,075 + BWI76_RS27245 0.70 -0.8
5,655,094 - BWI76_RS27245 0.72 -0.7
5,655,094 - BWI76_RS27245 0.72 -0.3
5,655,142 + BWI76_RS27245 0.78 -1.0
5,655,258 - -0.4
5,655,329 + -0.4
5,655,330 + -0.2
5,655,339 + +0.0
5,655,347 - +1.1
5,655,510 + +0.6
5,655,525 + +0.7
5,655,574 - +1.0
5,655,605 - BWI76_RS27250 0.12 +0.6
5,655,798 - BWI76_RS27250 0.31 +1.0
5,655,798 - BWI76_RS27250 0.31 +1.9
5,655,815 + -0.1
5,655,823 - BWI76_RS27250 0.34 +1.0
5,655,839 + BWI76_RS27250 0.35 +0.8
5,655,887 - BWI76_RS27250 0.40 +1.1
5,655,987 - BWI76_RS27250 0.50 +1.0
5,655,987 - BWI76_RS27250 0.50 +0.6
5,656,263 + BWI76_RS27250 0.77 +0.5
5,656,271 - BWI76_RS27250 0.77 +2.5
5,656,278 + BWI76_RS27250 0.78 -2.0
5,656,278 + BWI76_RS27250 0.78 -0.9
5,656,286 - BWI76_RS27250 0.79 +0.4
5,656,311 + BWI76_RS27250 0.81 +0.7
5,656,364 - BWI76_RS27250 0.86 +1.4
5,656,388 - BWI76_RS27250 0.89 -0.4
5,656,465 + +1.7
5,656,510 + +1.4
5,656,525 + +0.8
5,656,545 + +1.0
5,656,546 + +0.7
5,656,560 - -1.5
5,656,700 + BWI76_RS27255 0.16 +0.9
5,656,718 - BWI76_RS27255 0.17 +1.1
5,656,783 + BWI76_RS27255 0.23 +1.0
5,657,029 - BWI76_RS27255 0.43 +1.1
5,657,029 - BWI76_RS27255 0.43 +1.3
5,657,134 - BWI76_RS27255 0.52 +1.1
5,657,137 + BWI76_RS27255 0.52 +1.7
5,657,153 + BWI76_RS27255 0.54 +1.9
5,657,275 - BWI76_RS27255 0.64 +1.7
5,657,277 + BWI76_RS27255 0.64 +0.2
5,657,305 + BWI76_RS27255 0.66 +0.5
5,657,313 - BWI76_RS27255 0.67 +1.4
5,657,313 - BWI76_RS27255 0.67 +1.1
5,657,394 - BWI76_RS27255 0.74 +1.2
5,657,447 - +1.8

Or see this region's nucleotide sequence