Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25295

Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25285 and BWI76_RS25290 are separated by 53 nucleotidesBWI76_RS25290 and BWI76_RS25295 overlap by 4 nucleotidesBWI76_RS25295 and BWI76_RS25300 are separated by 234 nucleotides BWI76_RS25285: BWI76_RS25285 - HPr family phosphocarrier protein, at 5,255,041 to 5,255,313 _RS25285 BWI76_RS25290: BWI76_RS25290 - monofunctional biosynthetic peptidoglycan transglycosylase, at 5,255,367 to 5,256,092 _RS25290 BWI76_RS25295: BWI76_RS25295 - glutamine amidotransferase, at 5,256,089 to 5,256,742 _RS25295 BWI76_RS25300: BWI76_RS25300 - hybrid sensor histidine kinase/response regulator, at 5,256,977 to 5,259,316 _RS25300 Position (kb) 5256 5257Strain fitness (log2 ratio) -1 0 1 2at 5255.356 kb on - strandat 5255.396 kb on - strandat 5255.680 kb on - strand, within BWI76_RS25290at 5255.899 kb on - strand, within BWI76_RS25290at 5255.989 kb on + strand, within BWI76_RS25290at 5255.989 kb on + strand, within BWI76_RS25290at 5255.994 kb on + strand, within BWI76_RS25290at 5255.997 kb on - strand, within BWI76_RS25290at 5256.013 kb on + strand, within BWI76_RS25290at 5256.021 kb on - strandat 5256.347 kb on + strand, within BWI76_RS25295at 5256.468 kb on + strand, within BWI76_RS25295at 5256.521 kb on - strand, within BWI76_RS25295at 5256.556 kb on + strand, within BWI76_RS25295at 5256.724 kb on - strandat 5256.729 kb on - strandat 5256.745 kb on + strandat 5256.960 kb on + strandat 5257.183 kb on - strandat 5257.207 kb on - strandat 5257.453 kb on - strand, within BWI76_RS25300

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM
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5,255,356 - +0.9
5,255,396 - -0.4
5,255,680 - BWI76_RS25290 0.43 +0.6
5,255,899 - BWI76_RS25290 0.73 +0.9
5,255,989 + BWI76_RS25290 0.86 +0.6
5,255,989 + BWI76_RS25290 0.86 -1.4
5,255,994 + BWI76_RS25290 0.86 -1.3
5,255,997 - BWI76_RS25290 0.87 +0.1
5,256,013 + BWI76_RS25290 0.89 +0.1
5,256,021 - -0.7
5,256,347 + BWI76_RS25295 0.39 +1.8
5,256,468 + BWI76_RS25295 0.58 +0.5
5,256,521 - BWI76_RS25295 0.66 +0.4
5,256,556 + BWI76_RS25295 0.71 +0.4
5,256,724 - -0.5
5,256,729 - +0.6
5,256,745 + -0.3
5,256,960 + +0.1
5,257,183 - +1.0
5,257,207 - -1.7
5,257,453 - BWI76_RS25300 0.20 +0.1

Or see this region's nucleotide sequence