Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21540 and BWI76_RS21545 are separated by 65 nucleotides BWI76_RS21545 and BWI76_RS21550 overlap by 36 nucleotides BWI76_RS21550 and BWI76_RS21555 are separated by 13 nucleotides BWI76_RS21555 and BWI76_RS21560 are separated by 86 nucleotides
BWI76_RS21540: BWI76_RS21540 - membrane protein, at 4,510,017 to 4,511,306
_RS21540
BWI76_RS21545: BWI76_RS21545 - nucleotide exchange factor GrpE, at 4,511,372 to 4,511,962
_RS21545
BWI76_RS21550: BWI76_RS21550 - inorganic polyphosphate kinase, at 4,511,927 to 4,512,073
_RS21550
BWI76_RS21555: BWI76_RS21555 - NAD(+) kinase, at 4,512,087 to 4,512,965
_RS21555
BWI76_RS21560: BWI76_RS21560 - DNA repair protein RecN, at 4,513,052 to 4,514,713
_RS21560
Position (kb) 4512
4513 Strain fitness (log2 ratio) -1
0
1 at 4511.210 kb on + strand at 4511.218 kb on - strand at 4511.232 kb on + strand at 4513.141 kb on + strand at 4513.141 kb on + strand at 4513.823 kb on + strand, within BWI76_RS21560 at 4513.838 kb on - strand, within BWI76_RS21560
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM remove 4,511,210 + -0.9 4,511,218 - +0.1 4,511,232 + +0.3 4,513,141 + -0.6 4,513,141 + -0.2 4,513,823 + BWI76_RS21560 0.46 -1.3 4,513,838 - BWI76_RS21560 0.47 -0.6
Or see this region's nucleotide sequence