Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20185

Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20180 and BWI76_RS20185 are separated by 98 nucleotidesBWI76_RS20185 and BWI76_RS20190 are separated by 15 nucleotidesBWI76_RS20190 and BWI76_RS20195 overlap by 23 nucleotides BWI76_RS20180: BWI76_RS20180 - NADH-quinone oxidoreductase subunit C/D, at 4,228,967 to 4,230,763 _RS20180 BWI76_RS20185: BWI76_RS20185 - NADH-quinone oxidoreductase subunit B, at 4,230,862 to 4,231,536 _RS20185 BWI76_RS20190: BWI76_RS20190 - NADH-quinone oxidoreductase subunit A, at 4,231,552 to 4,231,998 _RS20190 BWI76_RS20195: BWI76_RS20195 - hypothetical protein, at 4,231,976 to 4,232,186 _RS20195 Position (kb) 4230 4231 4232Strain fitness (log2 ratio) -2 -1 0 1at 4230.095 kb on + strand, within BWI76_RS20180at 4230.096 kb on - strand, within BWI76_RS20180at 4230.263 kb on - strand, within BWI76_RS20180at 4230.307 kb on + strand, within BWI76_RS20180at 4230.870 kb on + strandat 4231.087 kb on + strand, within BWI76_RS20185at 4231.302 kb on - strandat 4231.557 kb on + strandat 4231.565 kb on - strandat 4231.591 kb on - strandat 4231.657 kb on - strand, within BWI76_RS20190at 4231.908 kb on + strand, within BWI76_RS20190at 4231.926 kb on - strand, within BWI76_RS20190at 4232.281 kb on - strandat 4232.334 kb on - strandat 4232.434 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM
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4,230,095 + BWI76_RS20180 0.63 +0.3
4,230,096 - BWI76_RS20180 0.63 +0.6
4,230,263 - BWI76_RS20180 0.72 +0.9
4,230,307 + BWI76_RS20180 0.75 -0.5
4,230,870 + -1.5
4,231,087 + BWI76_RS20185 0.33 -2.2
4,231,302 - +1.6
4,231,557 + -0.3
4,231,565 - +0.3
4,231,591 - +0.7
4,231,657 - BWI76_RS20190 0.23 -0.2
4,231,908 + BWI76_RS20190 0.80 -0.1
4,231,926 - BWI76_RS20190 0.84 -0.7
4,232,281 - -0.4
4,232,334 - -0.5
4,232,434 - -0.1

Or see this region's nucleotide sequence