Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18330

Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18310 and BWI76_RS18315 are separated by 13 nucleotidesBWI76_RS18315 and BWI76_RS18320 are separated by 38 nucleotidesBWI76_RS18320 and BWI76_RS18325 are separated by 151 nucleotidesBWI76_RS18325 and BWI76_RS18330 are separated by 57 nucleotidesBWI76_RS18330 and BWI76_RS18335 overlap by 4 nucleotides BWI76_RS18310: BWI76_RS18310 - tRNA-Leu, at 3,804,531 to 3,804,617 _RS18310 BWI76_RS18315: BWI76_RS18315 - tRNA-Cys, at 3,804,631 to 3,804,704 _RS18315 BWI76_RS18320: BWI76_RS18320 - tRNA-Gly, at 3,804,743 to 3,804,818 _RS18320 BWI76_RS18325: BWI76_RS18325 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,804,970 to 3,805,518 _RS18325 BWI76_RS18330: BWI76_RS18330 - excinuclease ABC subunit C, at 3,805,576 to 3,807,408 _RS18330 BWI76_RS18335: BWI76_RS18335 - DNA-binding response regulator, at 3,807,405 to 3,808,061 _RS18335 Position (kb) 3805 3806 3807 3808Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3805.581 kb on + strandat 3805.828 kb on - strand, within BWI76_RS18330at 3805.859 kb on - strand, within BWI76_RS18330at 3805.859 kb on - strand, within BWI76_RS18330at 3806.088 kb on + strand, within BWI76_RS18330at 3806.828 kb on - strand, within BWI76_RS18330at 3806.868 kb on - strand, within BWI76_RS18330at 3806.880 kb on + strand, within BWI76_RS18330at 3806.880 kb on + strand, within BWI76_RS18330at 3806.888 kb on - strand, within BWI76_RS18330at 3806.888 kb on - strand, within BWI76_RS18330at 3806.888 kb on - strand, within BWI76_RS18330at 3806.888 kb on - strand, within BWI76_RS18330at 3806.888 kb on - strand, within BWI76_RS18330at 3806.987 kb on - strand, within BWI76_RS18330at 3807.005 kb on + strand, within BWI76_RS18330at 3807.432 kb on + strandat 3807.435 kb on - strandat 3807.440 kb on - strandat 3807.467 kb on + strandat 3807.472 kb on - strand, within BWI76_RS18335at 3807.653 kb on + strand, within BWI76_RS18335at 3807.696 kb on - strand, within BWI76_RS18335at 3807.902 kb on - strand, within BWI76_RS18335at 3807.922 kb on - strand, within BWI76_RS18335at 3807.933 kb on + strand, within BWI76_RS18335at 3807.963 kb on - strand, within BWI76_RS18335at 3807.966 kb on - strand, within BWI76_RS18335at 3807.971 kb on - strand, within BWI76_RS18335at 3807.977 kb on + strand, within BWI76_RS18335at 3807.977 kb on + strand, within BWI76_RS18335at 3807.985 kb on - strand, within BWI76_RS18335at 3807.986 kb on + strand, within BWI76_RS18335at 3808.008 kb on + strandat 3808.016 kb on - strandat 3808.016 kb on - strandat 3808.018 kb on - strandat 3808.035 kb on - strandat 3808.045 kb on + strandat 3808.056 kb on + strandat 3808.056 kb on + strandat 3808.064 kb on - strandat 3808.064 kb on - strandat 3808.084 kb on + strandat 3808.093 kb on + strandat 3808.101 kb on - strandat 3808.200 kb on - strandat 3808.200 kb on - strandat 3808.219 kb on - strandat 3808.220 kb on + strandat 3808.220 kb on + strandat 3808.220 kb on + strandat 3808.225 kb on + strandat 3808.228 kb on - strandat 3808.228 kb on - strandat 3808.228 kb on - strandat 3808.228 kb on - strandat 3808.228 kb on - strandat 3808.228 kb on - strandat 3808.233 kb on - strandat 3808.276 kb on - strandat 3808.276 kb on - strandat 3808.283 kb on - strandat 3808.283 kb on - strandat 3808.283 kb on - strandat 3808.292 kb on + strandat 3808.295 kb on - strandat 3808.300 kb on - strandat 3808.300 kb on - strandat 3808.300 kb on - strandat 3808.302 kb on - strandat 3808.338 kb on - strandat 3808.360 kb on + strandat 3808.361 kb on + strandat 3808.361 kb on + strandat 3808.361 kb on + strandat 3808.361 kb on + strandat 3808.361 kb on + strandat 3808.369 kb on - strandat 3808.369 kb on - strandat 3808.369 kb on - strandat 3808.369 kb on - strandat 3808.369 kb on - strandat 3808.369 kb on - strandat 3808.369 kb on - strandat 3808.369 kb on - strandat 3808.380 kb on + strandat 3808.388 kb on - strandat 3808.407 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM
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3,805,581 + -0.1
3,805,828 - BWI76_RS18330 0.14 +0.4
3,805,859 - BWI76_RS18330 0.15 -0.8
3,805,859 - BWI76_RS18330 0.15 -0.4
3,806,088 + BWI76_RS18330 0.28 +0.1
3,806,828 - BWI76_RS18330 0.68 -0.2
3,806,868 - BWI76_RS18330 0.70 +0.1
3,806,880 + BWI76_RS18330 0.71 -3.8
3,806,880 + BWI76_RS18330 0.71 +0.5
3,806,888 - BWI76_RS18330 0.72 +0.1
3,806,888 - BWI76_RS18330 0.72 +0.1
3,806,888 - BWI76_RS18330 0.72 +0.2
3,806,888 - BWI76_RS18330 0.72 +0.1
3,806,888 - BWI76_RS18330 0.72 +0.6
3,806,987 - BWI76_RS18330 0.77 +0.3
3,807,005 + BWI76_RS18330 0.78 +0.0
3,807,432 + -1.5
3,807,435 - +0.7
3,807,440 - -2.3
3,807,467 + -3.5
3,807,472 - BWI76_RS18335 0.10 -3.9
3,807,653 + BWI76_RS18335 0.38 -1.7
3,807,696 - BWI76_RS18335 0.44 -1.5
3,807,902 - BWI76_RS18335 0.76 -2.7
3,807,922 - BWI76_RS18335 0.79 -0.6
3,807,933 + BWI76_RS18335 0.80 -3.5
3,807,963 - BWI76_RS18335 0.85 -0.0
3,807,966 - BWI76_RS18335 0.85 -3.2
3,807,971 - BWI76_RS18335 0.86 -1.9
3,807,977 + BWI76_RS18335 0.87 +1.0
3,807,977 + BWI76_RS18335 0.87 -2.1
3,807,985 - BWI76_RS18335 0.88 -2.9
3,807,986 + BWI76_RS18335 0.88 +0.2
3,808,008 + +0.4
3,808,016 - -0.9
3,808,016 - +2.0
3,808,018 - -2.1
3,808,035 - -1.8
3,808,045 + -1.2
3,808,056 + -0.3
3,808,056 + -2.9
3,808,064 - -3.9
3,808,064 - -2.0
3,808,084 + +2.0
3,808,093 + -0.3
3,808,101 - -0.0
3,808,200 - +2.0
3,808,200 - +1.0
3,808,219 - -3.1
3,808,220 + -0.5
3,808,220 + -0.6
3,808,220 + +0.5
3,808,225 + +1.3
3,808,228 - +0.3
3,808,228 - +1.0
3,808,228 - -0.2
3,808,228 - +0.7
3,808,228 - +3.0
3,808,228 - +0.5
3,808,233 - +1.7
3,808,276 - +1.3
3,808,276 - +1.1
3,808,283 - -2.8
3,808,283 - +2.8
3,808,283 - +1.7
3,808,292 + -2.5
3,808,295 - +1.1
3,808,300 - -1.1
3,808,300 - +0.4
3,808,300 - +0.4
3,808,302 - +1.0
3,808,338 - -0.4
3,808,360 + +1.2
3,808,361 + +2.3
3,808,361 + +0.5
3,808,361 + -0.2
3,808,361 + +0.7
3,808,361 + -2.4
3,808,369 - -0.4
3,808,369 - +2.6
3,808,369 - -0.2
3,808,369 - +1.2
3,808,369 - +1.9
3,808,369 - -2.7
3,808,369 - +0.0
3,808,369 - +0.7
3,808,380 + -0.6
3,808,388 - +0.2
3,808,407 + -0.7

Or see this region's nucleotide sequence