Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16735 and BWI76_RS16740 are separated by 48 nucleotides BWI76_RS16740 and BWI76_RS16745 are separated by 106 nucleotides BWI76_RS16745 and BWI76_RS16750 are separated by 10 nucleotides
BWI76_RS16735: BWI76_RS16735 - cold-shock protein, at 3,467,232 to 3,467,444
_RS16735
BWI76_RS16740: BWI76_RS16740 - transcriptional regulator, at 3,467,493 to 3,468,365
_RS16740
BWI76_RS16745: BWI76_RS16745 - alpha-acetolactate decarboxylase, at 3,468,472 to 3,469,251
_RS16745
BWI76_RS16750: BWI76_RS16750 - acetolactate synthase, at 3,469,262 to 3,470,941
_RS16750
Position (kb)
3467
3468
3469 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3466.498 kb on - strand at 3466.569 kb on - strand at 3466.757 kb on + strand at 3466.763 kb on + strand at 3466.776 kb on - strand at 3466.791 kb on + strand at 3466.799 kb on - strand at 3466.819 kb on + strand at 3466.837 kb on - strand at 3466.853 kb on + strand at 3466.861 kb on + strand at 3466.861 kb on + strand at 3466.869 kb on + strand at 3466.945 kb on + strand at 3466.947 kb on + strand at 3466.963 kb on - strand at 3466.963 kb on - strand at 3466.963 kb on - strand at 3466.964 kb on + strand at 3466.964 kb on + strand at 3466.965 kb on - strand at 3466.969 kb on + strand at 3466.969 kb on + strand at 3466.969 kb on + strand at 3466.972 kb on - strand at 3466.977 kb on - strand at 3467.021 kb on + strand at 3467.029 kb on - strand at 3467.031 kb on - strand at 3467.073 kb on + strand at 3467.080 kb on - strand at 3467.184 kb on - strand at 3467.191 kb on + strand at 3467.199 kb on - strand at 3467.199 kb on - strand at 3467.199 kb on - strand at 3467.201 kb on - strand at 3467.201 kb on - strand at 3467.286 kb on + strand, within BWI76_RS16735 at 3467.366 kb on - strand, within BWI76_RS16735 at 3467.368 kb on + strand, within BWI76_RS16735 at 3467.404 kb on - strand, within BWI76_RS16735 at 3467.406 kb on + strand, within BWI76_RS16735 at 3467.542 kb on - strand at 3467.583 kb on - strand, within BWI76_RS16740 at 3467.764 kb on - strand, within BWI76_RS16740 at 3468.038 kb on + strand, within BWI76_RS16740 at 3468.038 kb on + strand, within BWI76_RS16740 at 3468.205 kb on - strand at 3468.518 kb on - strand at 3468.524 kb on - strand at 3468.549 kb on - strand at 3468.559 kb on + strand, within BWI76_RS16745 at 3468.576 kb on - strand, within BWI76_RS16745 at 3468.576 kb on - strand, within BWI76_RS16745 at 3468.585 kb on + strand, within BWI76_RS16745 at 3468.585 kb on + strand, within BWI76_RS16745 at 3468.800 kb on + strand, within BWI76_RS16745 at 3469.030 kb on - strand, within BWI76_RS16745 at 3469.079 kb on + strand, within BWI76_RS16745 at 3469.307 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM remove 3,466,498 - -0.7 3,466,569 - -1.3 3,466,757 + -2.7 3,466,763 + +2.0 3,466,776 - +1.2 3,466,791 + +1.3 3,466,799 - +1.0 3,466,819 + +0.4 3,466,837 - -0.4 3,466,853 + +0.8 3,466,861 + +1.0 3,466,861 + -0.9 3,466,869 + +2.5 3,466,945 + -2.3 3,466,947 + +0.1 3,466,963 - -0.5 3,466,963 - +2.4 3,466,963 - +2.0 3,466,964 + -2.4 3,466,964 + -2.4 3,466,965 - +0.2 3,466,969 + -0.6 3,466,969 + -0.9 3,466,969 + -1.3 3,466,972 - -0.3 3,466,977 - -0.8 3,467,021 + -0.1 3,467,029 - +0.5 3,467,031 - -0.0 3,467,073 + -0.4 3,467,080 - -0.3 3,467,184 - -0.4 3,467,191 + +0.5 3,467,199 - -0.0 3,467,199 - -1.1 3,467,199 - +0.6 3,467,201 - +0.0 3,467,201 - +0.2 3,467,286 + BWI76_RS16735 0.25 +1.5 3,467,366 - BWI76_RS16735 0.63 +0.7 3,467,368 + BWI76_RS16735 0.64 -1.3 3,467,404 - BWI76_RS16735 0.81 -0.7 3,467,406 + BWI76_RS16735 0.82 +0.8 3,467,542 - +0.1 3,467,583 - BWI76_RS16740 0.10 +1.0 3,467,764 - BWI76_RS16740 0.31 -0.8 3,468,038 + BWI76_RS16740 0.62 +0.6 3,468,038 + BWI76_RS16740 0.62 +0.8 3,468,205 - -0.0 3,468,518 - +0.6 3,468,524 - +2.0 3,468,549 - +0.8 3,468,559 + BWI76_RS16745 0.11 +0.0 3,468,576 - BWI76_RS16745 0.13 -0.8 3,468,576 - BWI76_RS16745 0.13 +1.3 3,468,585 + BWI76_RS16745 0.14 +1.7 3,468,585 + BWI76_RS16745 0.14 +1.1 3,468,800 + BWI76_RS16745 0.42 -1.2 3,469,030 - BWI76_RS16745 0.72 +1.5 3,469,079 + BWI76_RS16745 0.78 +0.5 3,469,307 - -0.1
Or see this region's nucleotide sequence