Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13115

Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13110 and BWI76_RS13115 overlap by 4 nucleotidesBWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotidesBWI76_RS13120 and BWI76_RS13125 are separated by 3 nucleotides BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369 _RS13110 BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133 _RS13115 BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924 _RS13120 BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349 _RS13125 Position (kb) 2735 2736 2737Strain fitness (log2 ratio) -1 0 1at 2734.454 kb on + strand, within BWI76_RS13110at 2734.932 kb on - strand, within BWI76_RS13110at 2735.127 kb on - strand, within BWI76_RS13110at 2735.337 kb on + strandat 2735.375 kb on - strandat 2736.150 kb on - strandat 2736.166 kb on - strandat 2736.758 kb on + strand, within BWI76_RS13120at 2736.963 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM
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2,734,454 + BWI76_RS13110 0.14 +0.0
2,734,932 - BWI76_RS13110 0.59 -0.5
2,735,127 - BWI76_RS13110 0.77 -0.9
2,735,337 + -0.5
2,735,375 - +0.2
2,736,150 - +0.3
2,736,166 - -1.0
2,736,758 + BWI76_RS13120 0.79 -1.3
2,736,963 - +0.8

Or see this region's nucleotide sequence