Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2024 and Psest_2025 are separated by 61 nucleotides Psest_2025 and Psest_2026 are separated by 218 nucleotides Psest_2026 and Psest_2027 are separated by 29 nucleotides
Psest_2024: Psest_2024 - isocitrate dehydrogenase, NADP-dependent, prokaryotic type, at 2,144,486 to 2,145,742
_2024
Psest_2025: Psest_2025 - cold shock domain protein CspD, at 2,145,804 to 2,146,064
_2025
Psest_2026: Psest_2026 - Uncharacterized conserved protein, at 2,146,283 to 2,146,645
_2026
Psest_2027: Psest_2027 - ATP-dependent Clp protease ATP-binding subunit clpA, at 2,146,675 to 2,148,945
_2027
Position (kb)
2146
2147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2145.313 kb on + strand, within Psest_2024 at 2145.413 kb on + strand, within Psest_2024 at 2145.413 kb on + strand, within Psest_2024 at 2145.413 kb on + strand, within Psest_2024 at 2145.414 kb on - strand, within Psest_2024 at 2145.414 kb on - strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.538 kb on + strand, within Psest_2024 at 2145.539 kb on - strand, within Psest_2024 at 2145.539 kb on - strand, within Psest_2024 at 2145.539 kb on - strand, within Psest_2024 at 2145.600 kb on + strand, within Psest_2024 at 2145.601 kb on - strand, within Psest_2024 at 2145.662 kb on + strand at 2145.663 kb on - strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.740 kb on + strand at 2145.741 kb on - strand at 2146.008 kb on + strand, within Psest_2025 at 2146.059 kb on + strand at 2146.059 kb on + strand at 2146.060 kb on - strand at 2146.060 kb on - strand at 2146.101 kb on + strand at 2146.101 kb on + strand at 2146.101 kb on + strand at 2146.124 kb on + strand at 2146.167 kb on + strand at 2146.168 kb on - strand at 2146.175 kb on + strand at 2146.175 kb on + strand at 2146.176 kb on - strand at 2146.176 kb on - strand at 2146.184 kb on + strand at 2146.185 kb on - strand at 2146.241 kb on - strand at 2146.245 kb on - strand at 2146.245 kb on - strand at 2146.245 kb on - strand at 2146.245 kb on - strand at 2146.268 kb on - strand at 2146.308 kb on + strand at 2146.308 kb on + strand at 2146.308 kb on + strand at 2146.403 kb on + strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.420 kb on + strand, within Psest_2026 at 2146.421 kb on - strand, within Psest_2026 at 2146.431 kb on - strand, within Psest_2026 at 2146.547 kb on + strand, within Psest_2026 at 2146.547 kb on + strand, within Psest_2026 at 2146.548 kb on - strand, within Psest_2026 at 2146.592 kb on + strand, within Psest_2026 at 2146.592 kb on + strand, within Psest_2026 at 2146.593 kb on - strand, within Psest_2026 at 2146.674 kb on + strand at 2146.674 kb on + strand at 2146.674 kb on + strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.728 kb on + strand at 2146.728 kb on + strand at 2146.879 kb on - strand at 2147.083 kb on + strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.200 kb on + strand, within Psest_2027 at 2147.200 kb on + strand, within Psest_2027 at 2147.200 kb on + strand, within Psest_2027 at 2147.201 kb on - strand, within Psest_2027 at 2147.201 kb on - strand, within Psest_2027 at 2147.201 kb on - strand, within Psest_2027 at 2147.253 kb on - strand, within Psest_2027 at 2147.423 kb on - strand, within Psest_2027 at 2147.447 kb on + strand, within Psest_2027 at 2147.454 kb on + strand, within Psest_2027 at 2147.550 kb on + strand, within Psest_2027 at 2147.603 kb on + strand, within Psest_2027
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,145,313 + Psest_2024 0.66 -2.9 2,145,413 + Psest_2024 0.74 +1.6 2,145,413 + Psest_2024 0.74 +1.3 2,145,413 + Psest_2024 0.74 +0.2 2,145,414 - Psest_2024 0.74 +1.2 2,145,414 - Psest_2024 0.74 -0.5 2,145,538 + Psest_2024 0.84 +0.4 2,145,538 + Psest_2024 0.84 -0.2 2,145,538 + Psest_2024 0.84 -0.5 2,145,538 + Psest_2024 0.84 +3.1 2,145,538 + Psest_2024 0.84 -1.2 2,145,539 - Psest_2024 0.84 +0.4 2,145,539 - Psest_2024 0.84 -1.2 2,145,539 - Psest_2024 0.84 +0.2 2,145,600 + Psest_2024 0.89 +1.5 2,145,601 - Psest_2024 0.89 -0.8 2,145,662 + +0.2 2,145,663 - +0.4 2,145,736 + -1.2 2,145,736 + +0.1 2,145,736 + -0.0 2,145,736 + +0.8 2,145,736 + -0.1 2,145,736 + -0.4 2,145,736 + +2.2 2,145,736 + -1.1 2,145,737 - -1.3 2,145,737 - -1.4 2,145,737 - +0.0 2,145,737 - +0.2 2,145,737 - -1.4 2,145,737 - -0.6 2,145,740 + -1.0 2,145,741 - +0.1 2,146,008 + Psest_2025 0.78 +1.0 2,146,059 + -1.7 2,146,059 + -0.3 2,146,060 - +0.1 2,146,060 - +0.9 2,146,101 + -1.0 2,146,101 + +1.7 2,146,101 + +1.3 2,146,124 + +0.3 2,146,167 + +2.5 2,146,168 - -0.1 2,146,175 + -0.1 2,146,175 + +0.9 2,146,176 - +0.1 2,146,176 - +0.6 2,146,184 + +1.7 2,146,185 - -1.0 2,146,241 - -0.4 2,146,245 - +0.2 2,146,245 - -0.2 2,146,245 - -0.2 2,146,245 - -1.3 2,146,268 - -0.4 2,146,308 + +0.2 2,146,308 + -0.0 2,146,308 + +1.1 2,146,403 + Psest_2026 0.33 +0.1 2,146,404 - Psest_2026 0.33 +0.7 2,146,404 - Psest_2026 0.33 +0.2 2,146,404 - Psest_2026 0.33 -1.6 2,146,404 - Psest_2026 0.33 -1.6 2,146,404 - Psest_2026 0.33 -0.9 2,146,404 - Psest_2026 0.33 -1.6 2,146,405 + Psest_2026 0.34 -0.4 2,146,405 + Psest_2026 0.34 +1.2 2,146,405 + Psest_2026 0.34 +1.1 2,146,405 + Psest_2026 0.34 +0.1 2,146,405 + Psest_2026 0.34 +0.4 2,146,405 + Psest_2026 0.34 +0.6 2,146,405 + Psest_2026 0.34 +0.6 2,146,406 - Psest_2026 0.34 -1.2 2,146,406 - Psest_2026 0.34 +0.8 2,146,406 - Psest_2026 0.34 -2.5 2,146,406 - Psest_2026 0.34 -0.4 2,146,406 - Psest_2026 0.34 -2.6 2,146,406 - Psest_2026 0.34 -1.0 2,146,406 - Psest_2026 0.34 +0.1 2,146,406 - Psest_2026 0.34 -0.9 2,146,406 - Psest_2026 0.34 +0.8 2,146,420 + Psest_2026 0.38 +0.4 2,146,421 - Psest_2026 0.38 +0.4 2,146,431 - Psest_2026 0.41 -0.7 2,146,547 + Psest_2026 0.73 +1.6 2,146,547 + Psest_2026 0.73 -0.1 2,146,548 - Psest_2026 0.73 +0.0 2,146,592 + Psest_2026 0.85 +1.9 2,146,592 + Psest_2026 0.85 +1.1 2,146,593 - Psest_2026 0.85 -1.7 2,146,674 + -0.7 2,146,674 + -0.2 2,146,674 + +0.3 2,146,675 - +0.8 2,146,675 - -0.1 2,146,675 - -0.6 2,146,675 - -0.9 2,146,675 - +0.2 2,146,675 - +1.4 2,146,728 + +0.8 2,146,728 + +0.2 2,146,879 - -1.1 2,147,083 + Psest_2027 0.18 +0.3 2,147,084 - Psest_2027 0.18 -0.4 2,147,084 - Psest_2027 0.18 -0.2 2,147,084 - Psest_2027 0.18 -0.8 2,147,084 - Psest_2027 0.18 -3.0 2,147,084 - Psest_2027 0.18 -1.3 2,147,084 - Psest_2027 0.18 +0.2 2,147,200 + Psest_2027 0.23 -0.2 2,147,200 + Psest_2027 0.23 +0.3 2,147,200 + Psest_2027 0.23 +2.0 2,147,201 - Psest_2027 0.23 -2.7 2,147,201 - Psest_2027 0.23 +0.4 2,147,201 - Psest_2027 0.23 -2.3 2,147,253 - Psest_2027 0.25 +1.2 2,147,423 - Psest_2027 0.33 -1.2 2,147,447 + Psest_2027 0.34 +0.3 2,147,454 + Psest_2027 0.34 -0.9 2,147,550 + Psest_2027 0.39 -1.8 2,147,603 + Psest_2027 0.41 -0.7
Or see this region's nucleotide sequence