Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0181 and Psest_0182 are separated by 52 nucleotides Psest_0182 and Psest_0183 are separated by 59 nucleotides Psest_0183 and Psest_0184 are separated by 183 nucleotides
Psest_0181: Psest_0181 - glycine cleavage system T protein, at 175,363 to 176,445
_0181
Psest_0182: Psest_0182 - glycine cleavage system H protein, at 176,498 to 176,887
_0182
Psest_0183: Psest_0183 - glycine dehydrogenase (decarboxylating), at 176,947 to 179,823
_0183
Psest_0184: Psest_0184 - ribosome biogenesis GTP-binding protein YsxC/EngB, at 180,007 to 180,660
_0184
Position (kb) 176
177
178
179
180 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 176.307 kb on + strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.435 kb on + strand at 176.435 kb on + strand at 176.436 kb on - strand at 176.436 kb on - strand at 176.443 kb on + strand at 176.443 kb on + strand at 176.444 kb on - strand at 176.444 kb on - strand at 176.622 kb on - strand, within Psest_0182 at 176.622 kb on - strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.786 kb on + strand, within Psest_0182 at 176.940 kb on - strand at 176.940 kb on - strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.955 kb on + strand at 176.955 kb on + strand at 176.955 kb on + strand at 176.955 kb on + strand at 176.955 kb on + strand at 177.278 kb on - strand, within Psest_0183 at 177.278 kb on - strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.287 kb on - strand, within Psest_0183 at 177.287 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.803 kb on - strand, within Psest_0183 at 177.989 kb on - strand, within Psest_0183 at 177.989 kb on - strand, within Psest_0183 at 177.997 kb on + strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.700 kb on - strand, within Psest_0183 at 178.900 kb on + strand, within Psest_0183 at 178.901 kb on - strand, within Psest_0183 at 178.901 kb on - strand, within Psest_0183 at 178.901 kb on - strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.922 kb on - strand, within Psest_0183 at 178.922 kb on - strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.250 kb on + strand, within Psest_0183 at 179.311 kb on + strand, within Psest_0183 at 179.312 kb on - strand, within Psest_0183 at 179.312 kb on - strand, within Psest_0183 at 179.312 kb on - strand, within Psest_0183 at 179.312 kb on - strand, within Psest_0183 at 179.351 kb on - strand, within Psest_0183 at 179.458 kb on + strand, within Psest_0183 at 179.633 kb on + strand at 179.633 kb on + strand at 179.633 kb on + strand at 179.634 kb on - strand at 179.634 kb on - strand at 179.646 kb on + strand at 179.735 kb on - strand at 179.735 kb on - strand at 179.735 kb on - strand at 179.812 kb on + strand at 179.851 kb on + strand at 179.852 kb on - strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 180.008 kb on + strand at 180.009 kb on - strand at 180.009 kb on - strand at 180.009 kb on - strand at 180.722 kb on - strand at 180.722 kb on - strand at 180.722 kb on - strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.726 kb on - strand at 180.726 kb on - strand at 180.726 kb on - strand at 180.741 kb on - strand at 180.773 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 176,307 + Psest_0181 0.87 +1.6 176,308 - Psest_0181 0.87 -2.1 176,308 - Psest_0181 0.87 -0.8 176,308 - Psest_0181 0.87 -0.4 176,308 - Psest_0181 0.87 +1.7 176,308 - Psest_0181 0.87 -0.3 176,435 + +0.6 176,435 + -0.6 176,436 - -1.8 176,436 - -1.0 176,443 + +0.1 176,443 + -0.0 176,444 - +0.9 176,444 - +0.0 176,622 - Psest_0182 0.32 -0.6 176,622 - Psest_0182 0.32 -1.8 176,708 + Psest_0182 0.54 -0.3 176,708 + Psest_0182 0.54 +0.6 176,708 + Psest_0182 0.54 -1.1 176,708 + Psest_0182 0.54 -1.7 176,708 + Psest_0182 0.54 -1.2 176,708 + Psest_0182 0.54 -1.2 176,709 - Psest_0182 0.54 +0.0 176,709 - Psest_0182 0.54 -1.7 176,709 - Psest_0182 0.54 +0.5 176,709 - Psest_0182 0.54 -1.7 176,709 - Psest_0182 0.54 -0.6 176,709 - Psest_0182 0.54 -0.4 176,709 - Psest_0182 0.54 -0.1 176,709 - Psest_0182 0.54 -2.3 176,709 - Psest_0182 0.54 -0.2 176,786 + Psest_0182 0.74 -0.6 176,940 - -0.9 176,940 - +0.1 176,953 + +0.1 176,953 + +0.1 176,953 + +0.5 176,953 + -0.8 176,953 + -0.6 176,953 + +1.6 176,953 + +0.6 176,953 + -1.2 176,954 - -0.2 176,954 - +0.2 176,954 - -0.2 176,954 - -0.7 176,954 - -0.7 176,954 - +0.8 176,954 - -0.4 176,954 - -2.2 176,955 + -0.1 176,955 + -1.3 176,955 + -1.5 176,955 + -0.5 176,955 + -1.5 177,278 - Psest_0183 0.12 -2.0 177,278 - Psest_0183 0.12 -1.1 177,286 + Psest_0183 0.12 +0.6 177,286 + Psest_0183 0.12 +1.5 177,286 + Psest_0183 0.12 +0.3 177,286 + Psest_0183 0.12 +0.2 177,287 - Psest_0183 0.12 -0.6 177,287 - Psest_0183 0.12 -1.0 177,341 - Psest_0183 0.14 +1.1 177,341 - Psest_0183 0.14 -0.6 177,341 - Psest_0183 0.14 -1.0 177,341 - Psest_0183 0.14 +1.0 177,803 - Psest_0183 0.30 +0.5 177,989 - Psest_0183 0.36 +1.1 177,989 - Psest_0183 0.36 -0.0 177,997 + Psest_0183 0.36 -0.6 178,577 - Psest_0183 0.57 -0.8 178,577 - Psest_0183 0.57 -0.2 178,577 - Psest_0183 0.57 -2.0 178,577 - Psest_0183 0.57 -0.3 178,577 - Psest_0183 0.57 +1.1 178,700 - Psest_0183 0.61 -1.1 178,900 + Psest_0183 0.68 +0.6 178,901 - Psest_0183 0.68 +1.4 178,901 - Psest_0183 0.68 -1.5 178,901 - Psest_0183 0.68 +1.6 178,921 + Psest_0183 0.69 +1.7 178,921 + Psest_0183 0.69 +0.5 178,921 + Psest_0183 0.69 -1.7 178,921 + Psest_0183 0.69 +0.7 178,921 + Psest_0183 0.69 +0.1 178,922 - Psest_0183 0.69 -0.8 178,922 - Psest_0183 0.69 -0.3 178,978 + Psest_0183 0.71 -0.5 178,978 + Psest_0183 0.71 +1.3 178,978 + Psest_0183 0.71 -0.1 178,978 + Psest_0183 0.71 -1.0 178,978 + Psest_0183 0.71 -1.2 178,978 + Psest_0183 0.71 -1.2 178,978 + Psest_0183 0.71 +0.0 178,978 + Psest_0183 0.71 -2.2 178,978 + Psest_0183 0.71 -1.3 178,979 - Psest_0183 0.71 +1.2 178,979 - Psest_0183 0.71 -2.3 178,979 - Psest_0183 0.71 -0.2 178,979 - Psest_0183 0.71 +0.5 178,979 - Psest_0183 0.71 +0.5 178,979 - Psest_0183 0.71 -0.5 178,979 - Psest_0183 0.71 +2.1 178,979 - Psest_0183 0.71 -1.3 178,979 - Psest_0183 0.71 +2.1 178,979 - Psest_0183 0.71 +1.3 179,248 + Psest_0183 0.80 +0.1 179,248 + Psest_0183 0.80 +0.6 179,248 + Psest_0183 0.80 -0.2 179,248 + Psest_0183 0.80 -1.6 179,248 + Psest_0183 0.80 +0.0 179,248 + Psest_0183 0.80 +0.5 179,248 + Psest_0183 0.80 -0.1 179,248 + Psest_0183 0.80 -0.1 179,248 + Psest_0183 0.80 +2.0 179,248 + Psest_0183 0.80 +0.8 179,248 + Psest_0183 0.80 -1.0 179,248 + Psest_0183 0.80 -0.0 179,248 + Psest_0183 0.80 +1.8 179,248 + Psest_0183 0.80 -1.4 179,248 + Psest_0183 0.80 +0.9 179,249 - Psest_0183 0.80 +0.7 179,249 - Psest_0183 0.80 -2.0 179,249 - Psest_0183 0.80 +3.1 179,249 - Psest_0183 0.80 -1.0 179,249 - Psest_0183 0.80 -0.8 179,249 - Psest_0183 0.80 -0.3 179,249 - Psest_0183 0.80 +1.2 179,249 - Psest_0183 0.80 +0.0 179,249 - Psest_0183 0.80 -0.5 179,249 - Psest_0183 0.80 +0.1 179,249 - Psest_0183 0.80 +2.0 179,249 - Psest_0183 0.80 -1.4 179,249 - Psest_0183 0.80 -1.6 179,249 - Psest_0183 0.80 +0.2 179,249 - Psest_0183 0.80 -0.3 179,250 + Psest_0183 0.80 -1.1 179,311 + Psest_0183 0.82 +1.7 179,312 - Psest_0183 0.82 +1.2 179,312 - Psest_0183 0.82 -0.8 179,312 - Psest_0183 0.82 +1.6 179,312 - Psest_0183 0.82 -2.1 179,351 - Psest_0183 0.84 +1.7 179,458 + Psest_0183 0.87 -0.1 179,633 + -0.3 179,633 + -1.4 179,633 + -0.2 179,634 - -0.6 179,634 - -1.0 179,646 + -1.1 179,735 - +2.1 179,735 - -2.5 179,735 - +0.5 179,812 + -0.6 179,851 + -1.2 179,852 - +0.0 179,959 + +1.7 179,959 + -0.3 179,959 + -0.2 179,959 + -0.1 179,959 + +0.7 179,959 + -1.4 179,959 + -0.3 179,959 + +0.7 179,959 + -0.0 179,959 + -1.6 179,959 + +0.9 179,959 + -1.1 179,959 + -1.1 179,959 + -1.8 179,959 + -0.0 179,959 + +0.0 179,959 + +0.4 179,960 - -1.3 179,960 - -1.8 179,960 - +1.5 179,960 - -0.0 179,960 - -0.1 179,960 - +0.2 179,960 - +0.4 179,960 - +1.0 179,960 - +0.2 179,960 - -0.6 179,960 - -0.7 180,008 + +2.4 180,009 - -0.0 180,009 - -0.5 180,009 - -1.5 180,722 - -1.1 180,722 - +2.1 180,722 - -0.9 180,723 + +1.0 180,723 + +1.4 180,723 + +1.7 180,723 + -0.9 180,723 + +0.7 180,723 + +0.4 180,723 + -0.4 180,723 + -2.3 180,723 + -2.2 180,723 + +0.2 180,723 + -0.1 180,723 + +1.4 180,723 + +0.4 180,723 + -0.9 180,724 - -1.5 180,724 - +1.4 180,724 - +0.7 180,724 - -1.7 180,724 - +1.7 180,724 - +1.8 180,724 - +0.4 180,724 - +1.6 180,724 - -0.0 180,724 - +0.7 180,724 - -1.0 180,724 - +0.3 180,724 - +0.8 180,725 + +1.4 180,725 + +0.9 180,725 + -0.9 180,725 + -1.1 180,725 + +0.5 180,725 + -0.1 180,725 + +2.2 180,725 + +0.4 180,725 + -0.2 180,725 + +0.8 180,725 + -0.1 180,725 + +0.1 180,725 + +1.6 180,726 - -2.0 180,726 - -0.4 180,726 - +1.6 180,741 - +0.1 180,773 + +0.8
Or see this region's nucleotide sequence