Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS08165

Experiment: p-Coumaric (C) 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS30510 and PFLU_RS08160 are separated by 185 nucleotidesPFLU_RS08160 and PFLU_RS08165 overlap by 1 nucleotidesPFLU_RS08165 and PFLU_RS30515 are separated by 157 nucleotides PFLU_RS30510: PFLU_RS30510 - glycosyltransferase family 4 protein, at 1,822,674 to 1,823,891 _RS30510 PFLU_RS08160: PFLU_RS08160 - sugar transferase, at 1,824,077 to 1,824,721 _RS08160 PFLU_RS08165: PFLU_RS08165 - acyl carrier protein, at 1,824,721 to 1,824,927 _RS08165 PFLU_RS30515: PFLU_RS30515 - Rieske 2Fe-2S domain-containing protein, at 1,825,085 to 1,826,116 _RS30515 Position (kb) 1824 1825Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7at 1823.919 kb on - strandat 1823.921 kb on - strandat 1824.015 kb on + strandat 1824.053 kb on + strandat 1824.054 kb on - strandat 1824.141 kb on + strandat 1824.172 kb on + strand, within PFLU_RS08160at 1824.332 kb on - strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.340 kb on + strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.341 kb on - strand, within PFLU_RS08160at 1824.346 kb on + strand, within PFLU_RS08160at 1824.346 kb on + strand, within PFLU_RS08160at 1824.347 kb on - strand, within PFLU_RS08160at 1824.347 kb on - strand, within PFLU_RS08160at 1824.349 kb on + strand, within PFLU_RS08160at 1824.349 kb on + strand, within PFLU_RS08160at 1824.349 kb on + strand, within PFLU_RS08160at 1824.349 kb on + strand, within PFLU_RS08160at 1824.349 kb on + strand, within PFLU_RS08160at 1824.349 kb on + strand, within PFLU_RS08160at 1824.349 kb on + strand, within PFLU_RS08160at 1824.349 kb on + strand, within PFLU_RS08160at 1824.349 kb on + strand, within PFLU_RS08160at 1824.350 kb on - strand, within PFLU_RS08160at 1824.350 kb on - strand, within PFLU_RS08160at 1824.350 kb on - strand, within PFLU_RS08160at 1824.431 kb on + strand, within PFLU_RS08160at 1824.432 kb on - strand, within PFLU_RS08160at 1824.444 kb on - strand, within PFLU_RS08160at 1824.444 kb on - strand, within PFLU_RS08160at 1824.474 kb on + strand, within PFLU_RS08160at 1824.474 kb on + strand, within PFLU_RS08160at 1824.474 kb on + strand, within PFLU_RS08160at 1824.504 kb on + strand, within PFLU_RS08160at 1824.514 kb on + strand, within PFLU_RS08160at 1824.514 kb on + strand, within PFLU_RS08160at 1824.514 kb on + strand, within PFLU_RS08160at 1824.515 kb on - strand, within PFLU_RS08160at 1824.515 kb on - strand, within PFLU_RS08160at 1824.541 kb on + strand, within PFLU_RS08160at 1824.541 kb on + strand, within PFLU_RS08160at 1824.550 kb on + strand, within PFLU_RS08160at 1824.550 kb on + strand, within PFLU_RS08160at 1824.551 kb on - strand, within PFLU_RS08160at 1824.558 kb on - strand, within PFLU_RS08160at 1824.558 kb on - strand, within PFLU_RS08160at 1824.558 kb on - strand, within PFLU_RS08160at 1824.558 kb on - strand, within PFLU_RS08160at 1824.568 kb on + strand, within PFLU_RS08160at 1824.569 kb on - strand, within PFLU_RS08160at 1824.569 kb on - strand, within PFLU_RS08160at 1824.582 kb on + strand, within PFLU_RS08160at 1824.589 kb on - strand, within PFLU_RS08160at 1824.589 kb on - strand, within PFLU_RS08160at 1824.600 kb on + strand, within PFLU_RS08160at 1824.699 kb on + strandat 1824.699 kb on + strandat 1824.699 kb on + strandat 1824.703 kb on + strandat 1824.703 kb on + strandat 1824.703 kb on + strandat 1824.703 kb on + strandat 1824.704 kb on - strandat 1824.704 kb on - strandat 1824.836 kb on + strand, within PFLU_RS08165at 1824.836 kb on + strand, within PFLU_RS08165at 1824.839 kb on + strand, within PFLU_RS08165at 1824.839 kb on + strand, within PFLU_RS08165at 1824.840 kb on - strand, within PFLU_RS08165at 1824.840 kb on - strand, within PFLU_RS08165at 1824.915 kb on - strandat 1824.925 kb on + strandat 1824.926 kb on - strandat 1824.926 kb on - strandat 1824.929 kb on - strandat 1825.045 kb on + strandat 1825.045 kb on + strandat 1825.046 kb on - strandat 1825.068 kb on - strandat 1825.068 kb on - strandat 1825.075 kb on + strandat 1825.084 kb on + strandat 1825.215 kb on + strand, within PFLU_RS30515at 1825.322 kb on + strand, within PFLU_RS30515at 1825.355 kb on - strand, within PFLU_RS30515at 1825.355 kb on - strand, within PFLU_RS30515at 1825.422 kb on + strand, within PFLU_RS30515at 1825.422 kb on + strand, within PFLU_RS30515at 1825.426 kb on + strand, within PFLU_RS30515at 1825.429 kb on - strand, within PFLU_RS30515at 1825.469 kb on - strand, within PFLU_RS30515at 1825.607 kb on + strand, within PFLU_RS30515at 1825.613 kb on - strand, within PFLU_RS30515at 1825.781 kb on + strand, within PFLU_RS30515at 1825.795 kb on + strand, within PFLU_RS30515at 1825.796 kb on - strand, within PFLU_RS30515at 1825.889 kb on - strand, within PFLU_RS30515at 1825.915 kb on + strand, within PFLU_RS30515

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Per-strain Table

Position Strand Gene LocusTag Fraction p-Coumaric (C) 5 mM
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1,823,919 - +0.1
1,823,921 - +1.1
1,824,015 + -2.0
1,824,053 + -0.2
1,824,054 - +0.4
1,824,141 + -0.4
1,824,172 + PFLU_RS08160 0.15 +0.8
1,824,332 - PFLU_RS08160 0.40 +1.1
1,824,340 + PFLU_RS08160 0.41 -0.2
1,824,340 + PFLU_RS08160 0.41 -0.4
1,824,340 + PFLU_RS08160 0.41 -0.4
1,824,340 + PFLU_RS08160 0.41 +0.4
1,824,340 + PFLU_RS08160 0.41 +1.8
1,824,340 + PFLU_RS08160 0.41 +2.8
1,824,340 + PFLU_RS08160 0.41 +0.1
1,824,340 + PFLU_RS08160 0.41 +0.4
1,824,340 + PFLU_RS08160 0.41 +4.0
1,824,340 + PFLU_RS08160 0.41 -1.6
1,824,340 + PFLU_RS08160 0.41 +0.4
1,824,340 + PFLU_RS08160 0.41 +1.4
1,824,341 - PFLU_RS08160 0.41 +0.1
1,824,341 - PFLU_RS08160 0.41 -0.6
1,824,341 - PFLU_RS08160 0.41 +0.8
1,824,341 - PFLU_RS08160 0.41 +0.3
1,824,341 - PFLU_RS08160 0.41 -1.2
1,824,341 - PFLU_RS08160 0.41 +3.4
1,824,341 - PFLU_RS08160 0.41 -0.9
1,824,341 - PFLU_RS08160 0.41 +2.1
1,824,341 - PFLU_RS08160 0.41 -0.4
1,824,341 - PFLU_RS08160 0.41 +0.8
1,824,346 + PFLU_RS08160 0.42 +2.4
1,824,346 + PFLU_RS08160 0.42 +0.4
1,824,347 - PFLU_RS08160 0.42 +2.4
1,824,347 - PFLU_RS08160 0.42 +1.2
1,824,349 + PFLU_RS08160 0.42 +2.4
1,824,349 + PFLU_RS08160 0.42 -0.2
1,824,349 + PFLU_RS08160 0.42 +2.7
1,824,349 + PFLU_RS08160 0.42 -0.2
1,824,349 + PFLU_RS08160 0.42 +3.7
1,824,349 + PFLU_RS08160 0.42 +1.0
1,824,349 + PFLU_RS08160 0.42 +1.9
1,824,349 + PFLU_RS08160 0.42 +0.1
1,824,349 + PFLU_RS08160 0.42 +2.4
1,824,350 - PFLU_RS08160 0.42 +2.4
1,824,350 - PFLU_RS08160 0.42 +3.4
1,824,350 - PFLU_RS08160 0.42 +1.6
1,824,431 + PFLU_RS08160 0.55 -0.4
1,824,432 - PFLU_RS08160 0.55 +2.4
1,824,444 - PFLU_RS08160 0.57 +5.3
1,824,444 - PFLU_RS08160 0.57 +3.5
1,824,474 + PFLU_RS08160 0.62 +0.4
1,824,474 + PFLU_RS08160 0.62 +2.4
1,824,474 + PFLU_RS08160 0.62 -0.6
1,824,504 + PFLU_RS08160 0.66 -0.2
1,824,514 + PFLU_RS08160 0.68 +0.1
1,824,514 + PFLU_RS08160 0.68 -0.8
1,824,514 + PFLU_RS08160 0.68 +2.4
1,824,515 - PFLU_RS08160 0.68 +5.2
1,824,515 - PFLU_RS08160 0.68 +4.0
1,824,541 + PFLU_RS08160 0.72 -0.2
1,824,541 + PFLU_RS08160 0.72 +0.4
1,824,550 + PFLU_RS08160 0.73 -0.4
1,824,550 + PFLU_RS08160 0.73 +0.8
1,824,551 - PFLU_RS08160 0.73 +2.4
1,824,558 - PFLU_RS08160 0.75 +5.2
1,824,558 - PFLU_RS08160 0.75 +5.6
1,824,558 - PFLU_RS08160 0.75 +2.4
1,824,558 - PFLU_RS08160 0.75 -0.4
1,824,568 + PFLU_RS08160 0.76 +2.4
1,824,569 - PFLU_RS08160 0.76 +4.7
1,824,569 - PFLU_RS08160 0.76 +2.0
1,824,582 + PFLU_RS08160 0.78 -0.4
1,824,589 - PFLU_RS08160 0.79 +3.5
1,824,589 - PFLU_RS08160 0.79 +5.3
1,824,600 + PFLU_RS08160 0.81 +0.8
1,824,699 + -0.3
1,824,699 + -0.6
1,824,699 + -0.6
1,824,703 + -1.8
1,824,703 + +0.4
1,824,703 + +0.1
1,824,703 + +0.1
1,824,704 - +2.8
1,824,704 - +4.6
1,824,836 + PFLU_RS08165 0.56 +2.5
1,824,836 + PFLU_RS08165 0.56 +3.9
1,824,839 + PFLU_RS08165 0.57 +2.6
1,824,839 + PFLU_RS08165 0.57 +7.1
1,824,840 - PFLU_RS08165 0.57 -0.2
1,824,840 - PFLU_RS08165 0.57 +0.1
1,824,915 - +3.7
1,824,925 + -0.4
1,824,926 - +0.1
1,824,926 - -0.9
1,824,929 - -0.1
1,825,045 + -0.6
1,825,045 + -1.8
1,825,046 - +5.5
1,825,068 - +4.1
1,825,068 - +2.8
1,825,075 + +0.2
1,825,084 + +1.8
1,825,215 + PFLU_RS30515 0.13 +1.4
1,825,322 + PFLU_RS30515 0.23 +3.3
1,825,355 - PFLU_RS30515 0.26 +5.6
1,825,355 - PFLU_RS30515 0.26 +3.9
1,825,422 + PFLU_RS30515 0.33 +0.4
1,825,422 + PFLU_RS30515 0.33 +3.6
1,825,426 + PFLU_RS30515 0.33 +3.9
1,825,429 - PFLU_RS30515 0.33 +4.2
1,825,469 - PFLU_RS30515 0.37 +6.1
1,825,607 + PFLU_RS30515 0.51 +0.7
1,825,613 - PFLU_RS30515 0.51 +0.1
1,825,781 + PFLU_RS30515 0.67 +0.4
1,825,795 + PFLU_RS30515 0.69 -0.2
1,825,796 - PFLU_RS30515 0.69 +3.0
1,825,889 - PFLU_RS30515 0.78 +4.3
1,825,915 + PFLU_RS30515 0.80 +0.1

Or see this region's nucleotide sequence