Experiment: Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4954 and Psyr_4955 are separated by 27 nucleotides Psyr_4955 and Psyr_4956 overlap by 1 nucleotides Psyr_4956 and Psyr_4957 overlap by 37 nucleotides
Psyr_4954: Psyr_4954 - Protein of unknown function DUF877, at 5,871,865 to 5,873,367
_4954
Psyr_4955: Psyr_4955 - Protein of unknown function DUF1316, at 5,873,395 to 5,873,877
_4955
Psyr_4956: Psyr_4956 - protein of unknown function DUF879, at 5,873,877 to 5,875,709
_4956
Psyr_4957: Psyr_4957 - Protein of unknown function DUF1305, at 5,875,673 to 5,876,743
_4957
Position (kb) 5873
5874
5875
5876 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5872.955 kb on - strand, within Psyr_4954 at 5872.999 kb on + strand, within Psyr_4954 at 5872.999 kb on + strand, within Psyr_4954 at 5872.999 kb on + strand, within Psyr_4954 at 5872.999 kb on + strand, within Psyr_4954 at 5872.999 kb on + strand, within Psyr_4954 at 5873.000 kb on - strand, within Psyr_4954 at 5873.000 kb on - strand, within Psyr_4954 at 5873.000 kb on - strand, within Psyr_4954 at 5873.050 kb on + strand, within Psyr_4954 at 5873.050 kb on + strand, within Psyr_4954 at 5873.050 kb on + strand, within Psyr_4954 at 5873.050 kb on + strand, within Psyr_4954 at 5873.050 kb on + strand, within Psyr_4954 at 5873.050 kb on + strand, within Psyr_4954 at 5873.050 kb on + strand, within Psyr_4954 at 5873.050 kb on + strand, within Psyr_4954 at 5873.050 kb on + strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.051 kb on - strand, within Psyr_4954 at 5873.070 kb on + strand, within Psyr_4954 at 5873.080 kb on + strand, within Psyr_4954 at 5873.080 kb on + strand, within Psyr_4954 at 5873.080 kb on + strand, within Psyr_4954 at 5873.080 kb on + strand, within Psyr_4954 at 5873.081 kb on - strand, within Psyr_4954 at 5873.081 kb on - strand, within Psyr_4954 at 5873.081 kb on - strand, within Psyr_4954 at 5873.167 kb on + strand, within Psyr_4954 at 5873.167 kb on + strand, within Psyr_4954 at 5873.167 kb on + strand, within Psyr_4954 at 5873.167 kb on + strand, within Psyr_4954 at 5873.167 kb on + strand, within Psyr_4954 at 5873.167 kb on + strand, within Psyr_4954 at 5873.168 kb on - strand, within Psyr_4954 at 5873.168 kb on - strand, within Psyr_4954 at 5873.168 kb on - strand, within Psyr_4954 at 5873.168 kb on - strand, within Psyr_4954 at 5873.168 kb on - strand, within Psyr_4954 at 5873.168 kb on - strand, within Psyr_4954 at 5873.168 kb on - strand, within Psyr_4954 at 5873.269 kb on + strand at 5873.270 kb on - strand at 5873.284 kb on + strand at 5873.284 kb on + strand at 5873.284 kb on + strand at 5873.285 kb on - strand at 5873.285 kb on - strand at 5873.285 kb on - strand at 5873.285 kb on - strand at 5873.299 kb on + strand at 5873.299 kb on + strand at 5873.379 kb on + strand at 5873.380 kb on - strand at 5873.400 kb on + strand at 5873.400 kb on + strand at 5873.400 kb on + strand at 5873.401 kb on - strand at 5873.401 kb on - strand at 5873.401 kb on - strand at 5873.503 kb on + strand, within Psyr_4955 at 5873.503 kb on + strand, within Psyr_4955 at 5873.503 kb on + strand, within Psyr_4955 at 5873.504 kb on - strand, within Psyr_4955 at 5873.504 kb on - strand at 5873.611 kb on + strand, within Psyr_4955 at 5873.634 kb on + strand, within Psyr_4955 at 5873.786 kb on + strand, within Psyr_4955 at 5873.854 kb on + strand at 5873.854 kb on + strand at 5873.855 kb on - strand at 5873.855 kb on - strand at 5873.855 kb on - strand at 5873.898 kb on + strand at 5873.899 kb on - strand at 5873.899 kb on - strand at 5873.901 kb on + strand at 5873.901 kb on + strand at 5874.123 kb on + strand, within Psyr_4956 at 5874.123 kb on + strand, within Psyr_4956 at 5874.123 kb on + strand, within Psyr_4956 at 5874.123 kb on + strand, within Psyr_4956 at 5874.123 kb on + strand, within Psyr_4956 at 5874.123 kb on + strand, within Psyr_4956 at 5874.123 kb on + strand, within Psyr_4956 at 5874.124 kb on - strand, within Psyr_4956 at 5874.124 kb on - strand, within Psyr_4956 at 5874.124 kb on - strand, within Psyr_4956 at 5874.280 kb on + strand, within Psyr_4956 at 5874.280 kb on + strand, within Psyr_4956 at 5874.471 kb on + strand, within Psyr_4956 at 5874.471 kb on + strand, within Psyr_4956 at 5874.471 kb on + strand, within Psyr_4956 at 5874.471 kb on + strand, within Psyr_4956 at 5874.471 kb on + strand, within Psyr_4956 at 5874.471 kb on + strand, within Psyr_4956 at 5874.471 kb on + strand, within Psyr_4956 at 5874.471 kb on + strand, within Psyr_4956 at 5874.472 kb on - strand, within Psyr_4956 at 5874.472 kb on - strand, within Psyr_4956 at 5874.472 kb on - strand, within Psyr_4956 at 5874.472 kb on - strand, within Psyr_4956 at 5874.472 kb on - strand, within Psyr_4956 at 5874.472 kb on - strand, within Psyr_4956 at 5874.603 kb on + strand, within Psyr_4956 at 5874.645 kb on + strand, within Psyr_4956 at 5874.645 kb on + strand, within Psyr_4956 at 5874.645 kb on + strand, within Psyr_4956 at 5874.645 kb on + strand, within Psyr_4956 at 5874.646 kb on - strand, within Psyr_4956 at 5874.646 kb on - strand, within Psyr_4956 at 5874.741 kb on + strand, within Psyr_4956 at 5874.753 kb on + strand, within Psyr_4956 at 5874.753 kb on + strand, within Psyr_4956 at 5874.754 kb on - strand, within Psyr_4956 at 5874.754 kb on - strand, within Psyr_4956 at 5874.888 kb on + strand, within Psyr_4956 at 5874.889 kb on - strand, within Psyr_4956 at 5874.889 kb on - strand, within Psyr_4956 at 5875.325 kb on + strand, within Psyr_4956 at 5875.325 kb on + strand, within Psyr_4956 at 5875.326 kb on - strand, within Psyr_4956 at 5875.326 kb on - strand, within Psyr_4956 at 5875.401 kb on + strand, within Psyr_4956 at 5875.402 kb on - strand, within Psyr_4956 at 5875.402 kb on - strand, within Psyr_4956 at 5875.402 kb on - strand, within Psyr_4956 at 5875.549 kb on - strand at 5875.549 kb on - strand at 5875.606 kb on - strand at 5875.606 kb on - strand at 5875.606 kb on - strand at 5875.606 kb on - strand at 5875.633 kb on + strand at 5875.634 kb on - strand at 5875.778 kb on + strand at 5875.778 kb on + strand at 5875.779 kb on - strand at 5875.796 kb on - strand, within Psyr_4957 at 5876.001 kb on + strand, within Psyr_4957 at 5876.001 kb on + strand, within Psyr_4957 at 5876.002 kb on - strand, within Psyr_4957 at 5876.017 kb on + strand, within Psyr_4957 at 5876.017 kb on + strand, within Psyr_4957 at 5876.017 kb on + strand, within Psyr_4957 at 5876.017 kb on + strand, within Psyr_4957 at 5876.017 kb on + strand, within Psyr_4957 at 5876.017 kb on + strand, within Psyr_4957 at 5876.018 kb on - strand, within Psyr_4957 at 5876.018 kb on - strand, within Psyr_4957 at 5876.018 kb on - strand, within Psyr_4957 at 5876.018 kb on - strand, within Psyr_4957 at 5876.018 kb on - strand, within Psyr_4957 at 5876.105 kb on + strand, within Psyr_4957 at 5876.106 kb on - strand, within Psyr_4957 at 5876.106 kb on - strand, within Psyr_4957 at 5876.114 kb on + strand, within Psyr_4957 at 5876.115 kb on - strand, within Psyr_4957 at 5876.156 kb on + strand, within Psyr_4957 at 5876.156 kb on + strand, within Psyr_4957 at 5876.246 kb on + strand, within Psyr_4957 at 5876.246 kb on + strand, within Psyr_4957 at 5876.246 kb on + strand, within Psyr_4957 at 5876.247 kb on - strand, within Psyr_4957 at 5876.247 kb on - strand, within Psyr_4957 at 5876.312 kb on + strand, within Psyr_4957 at 5876.460 kb on + strand, within Psyr_4957 at 5876.460 kb on + strand, within Psyr_4957 at 5876.460 kb on + strand, within Psyr_4957 at 5876.460 kb on + strand, within Psyr_4957 at 5876.460 kb on + strand, within Psyr_4957 at 5876.460 kb on + strand, within Psyr_4957 at 5876.461 kb on - strand, within Psyr_4957 at 5876.461 kb on - strand, within Psyr_4957 at 5876.461 kb on - strand, within Psyr_4957 at 5876.461 kb on - strand, within Psyr_4957 at 5876.461 kb on - strand, within Psyr_4957 at 5876.461 kb on - strand, within Psyr_4957 at 5876.461 kb on - strand, within Psyr_4957 at 5876.465 kb on - strand, within Psyr_4957 at 5876.465 kb on - strand, within Psyr_4957 at 5876.555 kb on + strand, within Psyr_4957 at 5876.556 kb on - strand, within Psyr_4957 at 5876.556 kb on - strand, within Psyr_4957 at 5876.610 kb on + strand, within Psyr_4957 at 5876.643 kb on + strand at 5876.644 kb on - strand at 5876.644 kb on - strand at 5876.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant remove 5,872,955 - Psyr_4954 0.73 -0.1 5,872,999 + Psyr_4954 0.75 +1.9 5,872,999 + Psyr_4954 0.75 -2.8 5,872,999 + Psyr_4954 0.75 -1.5 5,872,999 + Psyr_4954 0.75 -1.2 5,872,999 + Psyr_4954 0.75 +1.5 5,873,000 - Psyr_4954 0.76 +1.5 5,873,000 - Psyr_4954 0.76 +0.5 5,873,000 - Psyr_4954 0.76 -0.2 5,873,050 + Psyr_4954 0.79 -1.3 5,873,050 + Psyr_4954 0.79 +0.5 5,873,050 + Psyr_4954 0.79 -0.0 5,873,050 + Psyr_4954 0.79 +1.2 5,873,050 + Psyr_4954 0.79 -0.9 5,873,050 + Psyr_4954 0.79 +0.1 5,873,050 + Psyr_4954 0.79 +0.9 5,873,050 + Psyr_4954 0.79 +0.2 5,873,050 + Psyr_4954 0.79 -0.5 5,873,051 - Psyr_4954 0.79 -2.4 5,873,051 - Psyr_4954 0.79 -0.5 5,873,051 - Psyr_4954 0.79 -1.7 5,873,051 - Psyr_4954 0.79 -0.5 5,873,051 - Psyr_4954 0.79 -0.0 5,873,051 - Psyr_4954 0.79 -1.1 5,873,051 - Psyr_4954 0.79 +1.4 5,873,051 - Psyr_4954 0.79 +0.3 5,873,051 - Psyr_4954 0.79 -0.3 5,873,051 - Psyr_4954 0.79 -0.1 5,873,051 - Psyr_4954 0.79 -1.5 5,873,051 - Psyr_4954 0.79 +0.4 5,873,051 - Psyr_4954 0.79 -0.6 5,873,051 - Psyr_4954 0.79 +0.5 5,873,051 - Psyr_4954 0.79 -0.5 5,873,051 - Psyr_4954 0.79 +0.1 5,873,051 - Psyr_4954 0.79 +0.8 5,873,051 - Psyr_4954 0.79 +0.3 5,873,051 - Psyr_4954 0.79 +0.2 5,873,051 - Psyr_4954 0.79 +1.2 5,873,051 - Psyr_4954 0.79 -0.5 5,873,070 + Psyr_4954 0.80 -0.2 5,873,080 + Psyr_4954 0.81 -0.2 5,873,080 + Psyr_4954 0.81 -0.7 5,873,080 + Psyr_4954 0.81 +1.7 5,873,080 + Psyr_4954 0.81 +0.3 5,873,081 - Psyr_4954 0.81 -0.1 5,873,081 - Psyr_4954 0.81 +1.4 5,873,081 - Psyr_4954 0.81 -0.6 5,873,167 + Psyr_4954 0.87 +1.3 5,873,167 + Psyr_4954 0.87 +0.4 5,873,167 + Psyr_4954 0.87 +1.0 5,873,167 + Psyr_4954 0.87 +0.8 5,873,167 + Psyr_4954 0.87 +1.8 5,873,167 + Psyr_4954 0.87 +1.0 5,873,168 - Psyr_4954 0.87 +1.1 5,873,168 - Psyr_4954 0.87 -0.0 5,873,168 - Psyr_4954 0.87 -0.0 5,873,168 - Psyr_4954 0.87 -0.2 5,873,168 - Psyr_4954 0.87 +0.2 5,873,168 - Psyr_4954 0.87 -2.7 5,873,168 - Psyr_4954 0.87 -0.4 5,873,269 + -0.0 5,873,270 - +1.8 5,873,284 + +0.2 5,873,284 + -2.3 5,873,284 + +1.6 5,873,285 - -0.3 5,873,285 - -0.5 5,873,285 - -1.8 5,873,285 - -0.4 5,873,299 + +0.3 5,873,299 + +1.6 5,873,379 + -0.2 5,873,380 - -1.2 5,873,400 + -0.5 5,873,400 + +1.2 5,873,400 + +0.9 5,873,401 - -0.4 5,873,401 - -0.0 5,873,401 - -0.1 5,873,503 + Psyr_4955 0.22 -0.9 5,873,503 + Psyr_4955 0.22 -0.1 5,873,503 + Psyr_4955 0.22 +1.9 5,873,504 - Psyr_4955 0.23 +0.5 5,873,504 - -0.9 5,873,611 + Psyr_4955 0.45 +2.0 5,873,634 + Psyr_4955 0.49 +0.6 5,873,786 + Psyr_4955 0.81 +1.6 5,873,854 + +1.0 5,873,854 + -0.5 5,873,855 - +0.3 5,873,855 - +0.5 5,873,855 - +0.4 5,873,898 + -1.2 5,873,899 - +0.6 5,873,899 - -1.6 5,873,901 + +1.0 5,873,901 + +0.4 5,874,123 + Psyr_4956 0.13 -1.4 5,874,123 + Psyr_4956 0.13 +1.9 5,874,123 + Psyr_4956 0.13 +1.2 5,874,123 + Psyr_4956 0.13 +0.5 5,874,123 + Psyr_4956 0.13 -0.3 5,874,123 + Psyr_4956 0.13 +0.0 5,874,123 + Psyr_4956 0.13 +0.0 5,874,124 - Psyr_4956 0.13 +1.8 5,874,124 - Psyr_4956 0.13 +0.4 5,874,124 - Psyr_4956 0.13 -1.2 5,874,280 + Psyr_4956 0.22 -0.6 5,874,280 + Psyr_4956 0.22 -1.4 5,874,471 + Psyr_4956 0.32 +0.3 5,874,471 + Psyr_4956 0.32 +0.0 5,874,471 + Psyr_4956 0.32 +2.0 5,874,471 + Psyr_4956 0.32 -1.3 5,874,471 + Psyr_4956 0.32 +0.3 5,874,471 + Psyr_4956 0.32 +0.3 5,874,471 + Psyr_4956 0.32 +0.5 5,874,471 + Psyr_4956 0.32 +0.8 5,874,472 - Psyr_4956 0.32 +0.2 5,874,472 - Psyr_4956 0.32 -0.2 5,874,472 - Psyr_4956 0.32 +0.3 5,874,472 - Psyr_4956 0.32 -0.5 5,874,472 - Psyr_4956 0.32 -0.1 5,874,472 - Psyr_4956 0.32 -1.2 5,874,603 + Psyr_4956 0.40 +1.4 5,874,645 + Psyr_4956 0.42 +0.3 5,874,645 + Psyr_4956 0.42 +1.0 5,874,645 + Psyr_4956 0.42 -0.1 5,874,645 + Psyr_4956 0.42 +0.1 5,874,646 - Psyr_4956 0.42 +0.0 5,874,646 - Psyr_4956 0.42 +0.2 5,874,741 + Psyr_4956 0.47 +1.6 5,874,753 + Psyr_4956 0.48 -0.2 5,874,753 + Psyr_4956 0.48 +0.5 5,874,754 - Psyr_4956 0.48 +0.5 5,874,754 - Psyr_4956 0.48 +0.8 5,874,888 + Psyr_4956 0.55 -0.3 5,874,889 - Psyr_4956 0.55 -0.1 5,874,889 - Psyr_4956 0.55 -0.3 5,875,325 + Psyr_4956 0.79 -1.9 5,875,325 + Psyr_4956 0.79 +0.4 5,875,326 - Psyr_4956 0.79 +1.4 5,875,326 - Psyr_4956 0.79 +0.6 5,875,401 + Psyr_4956 0.83 +0.2 5,875,402 - Psyr_4956 0.83 +0.4 5,875,402 - Psyr_4956 0.83 -0.4 5,875,402 - Psyr_4956 0.83 -0.4 5,875,549 - +0.8 5,875,549 - +0.4 5,875,606 - -1.6 5,875,606 - +1.8 5,875,606 - +1.2 5,875,606 - +0.8 5,875,633 + -0.5 5,875,634 - -1.2 5,875,778 + -0.6 5,875,778 + +0.3 5,875,779 - +1.1 5,875,796 - Psyr_4957 0.11 +0.8 5,876,001 + Psyr_4957 0.31 -0.9 5,876,001 + Psyr_4957 0.31 +1.2 5,876,002 - Psyr_4957 0.31 +1.6 5,876,017 + Psyr_4957 0.32 -1.2 5,876,017 + Psyr_4957 0.32 +0.8 5,876,017 + Psyr_4957 0.32 -0.5 5,876,017 + Psyr_4957 0.32 -0.3 5,876,017 + Psyr_4957 0.32 -0.1 5,876,017 + Psyr_4957 0.32 +0.5 5,876,018 - Psyr_4957 0.32 +0.1 5,876,018 - Psyr_4957 0.32 -0.2 5,876,018 - Psyr_4957 0.32 -1.2 5,876,018 - Psyr_4957 0.32 -0.6 5,876,018 - Psyr_4957 0.32 -2.0 5,876,105 + Psyr_4957 0.40 +0.2 5,876,106 - Psyr_4957 0.40 +0.8 5,876,106 - Psyr_4957 0.40 +0.9 5,876,114 + Psyr_4957 0.41 +0.7 5,876,115 - Psyr_4957 0.41 -0.5 5,876,156 + Psyr_4957 0.45 -1.0 5,876,156 + Psyr_4957 0.45 +1.0 5,876,246 + Psyr_4957 0.54 -0.2 5,876,246 + Psyr_4957 0.54 +0.6 5,876,246 + Psyr_4957 0.54 +0.2 5,876,247 - Psyr_4957 0.54 +1.6 5,876,247 - Psyr_4957 0.54 +0.3 5,876,312 + Psyr_4957 0.60 -0.2 5,876,460 + Psyr_4957 0.73 -0.4 5,876,460 + Psyr_4957 0.73 -1.2 5,876,460 + Psyr_4957 0.73 -1.0 5,876,460 + Psyr_4957 0.73 +0.4 5,876,460 + Psyr_4957 0.73 +0.4 5,876,460 + Psyr_4957 0.73 +1.1 5,876,461 - Psyr_4957 0.74 +0.3 5,876,461 - Psyr_4957 0.74 -0.8 5,876,461 - Psyr_4957 0.74 -0.2 5,876,461 - Psyr_4957 0.74 +0.6 5,876,461 - Psyr_4957 0.74 -1.8 5,876,461 - Psyr_4957 0.74 -0.2 5,876,461 - Psyr_4957 0.74 +0.6 5,876,465 - Psyr_4957 0.74 -0.3 5,876,465 - Psyr_4957 0.74 +0.8 5,876,555 + Psyr_4957 0.82 -0.9 5,876,556 - Psyr_4957 0.82 +1.0 5,876,556 - Psyr_4957 0.82 +0.1 5,876,610 + Psyr_4957 0.87 +0.3 5,876,643 + -0.2 5,876,644 - +0.5 5,876,644 - +0.2 5,876,644 - +0.4
Or see this region's nucleotide sequence