Experiment: Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3820 and Psyr_3821 are separated by 78 nucleotides Psyr_3821 and Psyr_3822 are separated by 1 nucleotides
Psyr_3820: Psyr_3820 - conserved domain protein, at 4,545,277 to 4,545,822
_3820
Psyr_3821: Psyr_3821 - Short-chain dehydrogenase/reductase SDR, at 4,545,901 to 4,546,635
_3821
Psyr_3822: Psyr_3822 - Protein of unknown function DUF6, at 4,546,637 to 4,547,515
_3822
Position (kb)
4545
4546
4547 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4544.908 kb on + strand at 4544.909 kb on - strand at 4544.962 kb on + strand at 4544.990 kb on - strand at 4545.092 kb on - strand at 4545.129 kb on + strand at 4545.140 kb on + strand at 4545.140 kb on + strand at 4545.140 kb on + strand at 4545.140 kb on + strand at 4545.141 kb on - strand at 4545.147 kb on + strand at 4545.147 kb on + strand at 4545.148 kb on - strand at 4545.553 kb on + strand, within Psyr_3820 at 4545.553 kb on + strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4545.554 kb on - strand, within Psyr_3820 at 4546.210 kb on + strand, within Psyr_3821 at 4546.210 kb on + strand, within Psyr_3821 at 4546.211 kb on - strand, within Psyr_3821 at 4546.363 kb on + strand, within Psyr_3821 at 4546.464 kb on - strand, within Psyr_3821 at 4546.468 kb on - strand, within Psyr_3821 at 4546.561 kb on + strand, within Psyr_3821 at 4546.561 kb on + strand, within Psyr_3821 at 4546.561 kb on + strand, within Psyr_3821 at 4546.561 kb on + strand, within Psyr_3821 at 4546.561 kb on + strand, within Psyr_3821 at 4546.561 kb on + strand, within Psyr_3821 at 4546.561 kb on + strand, within Psyr_3821 at 4546.562 kb on - strand at 4546.562 kb on - strand at 4546.562 kb on - strand at 4546.562 kb on - strand at 4546.562 kb on - strand at 4546.562 kb on - strand at 4546.588 kb on + strand at 4546.588 kb on + strand at 4546.589 kb on - strand at 4546.589 kb on - strand at 4546.843 kb on + strand, within Psyr_3822 at 4546.844 kb on - strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.854 kb on + strand, within Psyr_3822 at 4546.855 kb on - strand, within Psyr_3822 at 4546.855 kb on - strand, within Psyr_3822 at 4546.889 kb on + strand, within Psyr_3822 at 4546.890 kb on - strand, within Psyr_3822 at 4546.890 kb on - strand, within Psyr_3822 at 4546.983 kb on + strand, within Psyr_3822 at 4546.983 kb on + strand, within Psyr_3822 at 4546.983 kb on + strand, within Psyr_3822 at 4546.983 kb on + strand, within Psyr_3822 at 4546.984 kb on - strand, within Psyr_3822 at 4546.984 kb on - strand, within Psyr_3822 at 4546.984 kb on - strand, within Psyr_3822 at 4546.984 kb on - strand, within Psyr_3822 at 4546.990 kb on - strand, within Psyr_3822 at 4547.004 kb on + strand, within Psyr_3822 at 4547.046 kb on + strand, within Psyr_3822 at 4547.046 kb on + strand, within Psyr_3822 at 4547.046 kb on + strand, within Psyr_3822 at 4547.047 kb on - strand, within Psyr_3822 at 4547.047 kb on - strand, within Psyr_3822 at 4547.067 kb on + strand, within Psyr_3822 at 4547.067 kb on + strand, within Psyr_3822 at 4547.068 kb on - strand, within Psyr_3822 at 4547.068 kb on - strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.301 kb on + strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.302 kb on - strand, within Psyr_3822 at 4547.430 kb on + strand at 4547.430 kb on + strand at 4547.430 kb on + strand at 4547.430 kb on + strand at 4547.430 kb on + strand at 4547.431 kb on - strand at 4547.431 kb on - strand at 4547.431 kb on - strand at 4547.431 kb on - strand at 4547.454 kb on + strand at 4547.454 kb on + strand at 4547.455 kb on - strand at 4547.455 kb on - strand at 4547.504 kb on + strand at 4547.508 kb on + strand at 4547.547 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant remove 4,544,908 + +0.8 4,544,909 - -0.2 4,544,962 + -0.0 4,544,990 - +1.0 4,545,092 - +0.0 4,545,129 + +0.8 4,545,140 + +0.5 4,545,140 + -0.4 4,545,140 + +0.2 4,545,140 + -0.0 4,545,141 - +0.3 4,545,147 + -0.2 4,545,147 + -1.0 4,545,148 - -0.5 4,545,553 + Psyr_3820 0.51 -2.3 4,545,553 + Psyr_3820 0.51 +0.3 4,545,554 - Psyr_3820 0.51 -0.4 4,545,554 - Psyr_3820 0.51 +0.1 4,545,554 - Psyr_3820 0.51 +0.9 4,545,554 - Psyr_3820 0.51 -0.2 4,545,554 - Psyr_3820 0.51 +0.2 4,545,554 - Psyr_3820 0.51 -1.1 4,546,210 + Psyr_3821 0.42 -1.1 4,546,210 + Psyr_3821 0.42 -0.8 4,546,211 - Psyr_3821 0.42 -0.1 4,546,363 + Psyr_3821 0.63 -0.2 4,546,464 - Psyr_3821 0.77 -1.2 4,546,468 - Psyr_3821 0.77 -0.7 4,546,561 + Psyr_3821 0.90 -0.5 4,546,561 + Psyr_3821 0.90 -0.8 4,546,561 + Psyr_3821 0.90 +0.4 4,546,561 + Psyr_3821 0.90 -0.3 4,546,561 + Psyr_3821 0.90 +0.4 4,546,561 + Psyr_3821 0.90 -0.3 4,546,561 + Psyr_3821 0.90 -0.3 4,546,562 - -0.5 4,546,562 - +0.3 4,546,562 - +0.4 4,546,562 - -1.2 4,546,562 - +0.2 4,546,562 - +1.1 4,546,588 + +0.4 4,546,588 + +0.1 4,546,589 - -0.0 4,546,589 - +0.5 4,546,843 + Psyr_3822 0.23 +0.8 4,546,844 - Psyr_3822 0.24 -0.0 4,546,854 + Psyr_3822 0.25 -1.0 4,546,854 + Psyr_3822 0.25 -0.3 4,546,854 + Psyr_3822 0.25 +0.3 4,546,854 + Psyr_3822 0.25 +0.2 4,546,854 + Psyr_3822 0.25 -0.7 4,546,854 + Psyr_3822 0.25 -0.9 4,546,854 + Psyr_3822 0.25 +1.5 4,546,854 + Psyr_3822 0.25 +0.1 4,546,854 + Psyr_3822 0.25 -0.0 4,546,854 + Psyr_3822 0.25 +1.7 4,546,854 + Psyr_3822 0.25 -0.4 4,546,854 + Psyr_3822 0.25 -1.0 4,546,854 + Psyr_3822 0.25 -0.7 4,546,855 - Psyr_3822 0.25 -0.1 4,546,855 - Psyr_3822 0.25 +0.5 4,546,889 + Psyr_3822 0.29 -0.4 4,546,890 - Psyr_3822 0.29 -0.9 4,546,890 - Psyr_3822 0.29 -0.5 4,546,983 + Psyr_3822 0.39 +0.5 4,546,983 + Psyr_3822 0.39 -1.6 4,546,983 + Psyr_3822 0.39 +1.0 4,546,983 + Psyr_3822 0.39 +0.4 4,546,984 - Psyr_3822 0.39 -1.8 4,546,984 - Psyr_3822 0.39 -0.2 4,546,984 - Psyr_3822 0.39 +0.7 4,546,984 - Psyr_3822 0.39 +0.2 4,546,990 - Psyr_3822 0.40 -0.3 4,547,004 + Psyr_3822 0.42 -0.4 4,547,046 + Psyr_3822 0.47 +0.3 4,547,046 + Psyr_3822 0.47 -1.6 4,547,046 + Psyr_3822 0.47 -2.3 4,547,047 - Psyr_3822 0.47 -0.4 4,547,047 - Psyr_3822 0.47 +0.2 4,547,067 + Psyr_3822 0.49 +0.1 4,547,067 + Psyr_3822 0.49 +1.0 4,547,068 - Psyr_3822 0.49 +0.0 4,547,068 - Psyr_3822 0.49 -0.8 4,547,301 + Psyr_3822 0.76 -0.8 4,547,301 + Psyr_3822 0.76 -0.4 4,547,301 + Psyr_3822 0.76 +2.1 4,547,301 + Psyr_3822 0.76 -0.2 4,547,301 + Psyr_3822 0.76 +0.7 4,547,301 + Psyr_3822 0.76 -1.1 4,547,301 + Psyr_3822 0.76 -1.2 4,547,301 + Psyr_3822 0.76 -0.1 4,547,301 + Psyr_3822 0.76 +1.2 4,547,301 + Psyr_3822 0.76 -0.3 4,547,301 + Psyr_3822 0.76 +0.4 4,547,301 + Psyr_3822 0.76 +0.5 4,547,301 + Psyr_3822 0.76 +2.5 4,547,301 + Psyr_3822 0.76 +0.1 4,547,302 - Psyr_3822 0.76 -0.1 4,547,302 - Psyr_3822 0.76 +1.8 4,547,302 - Psyr_3822 0.76 +0.5 4,547,302 - Psyr_3822 0.76 +0.6 4,547,302 - Psyr_3822 0.76 +2.7 4,547,302 - Psyr_3822 0.76 -1.9 4,547,302 - Psyr_3822 0.76 -0.9 4,547,302 - Psyr_3822 0.76 +0.1 4,547,302 - Psyr_3822 0.76 +0.1 4,547,302 - Psyr_3822 0.76 -0.1 4,547,302 - Psyr_3822 0.76 +0.5 4,547,302 - Psyr_3822 0.76 -1.3 4,547,302 - Psyr_3822 0.76 +0.2 4,547,302 - Psyr_3822 0.76 +0.7 4,547,302 - Psyr_3822 0.76 +0.5 4,547,302 - Psyr_3822 0.76 +1.1 4,547,302 - Psyr_3822 0.76 +0.6 4,547,430 + +0.4 4,547,430 + +1.3 4,547,430 + +0.0 4,547,430 + -0.3 4,547,430 + +0.5 4,547,431 - +0.9 4,547,431 - +0.8 4,547,431 - +0.3 4,547,431 - +0.8 4,547,454 + -0.6 4,547,454 + -0.1 4,547,455 - -0.9 4,547,455 - +0.1 4,547,504 + -0.2 4,547,508 + -0.9 4,547,547 + -0.8
Or see this region's nucleotide sequence