Experiment: Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3045 and Psyr_3046 overlap by 4 nucleotides Psyr_3046 and Psyr_3047 are separated by 284 nucleotides Psyr_3047 and Psyr_3048 are separated by 30 nucleotides
Psyr_3045: Psyr_3045 - PqiB family protein, at 3,642,785 to 3,644,464
_3045
Psyr_3046: Psyr_3046 - Protein of unknown function DUF330, at 3,644,461 to 3,645,084
_3046
Psyr_3047: Psyr_3047 - Helix-turn-helix motif protein, at 3,645,369 to 3,645,818
_3047
Psyr_3048: Psyr_3048 - transcriptional regulator, LysR family, at 3,645,849 to 3,646,760
_3048
Position (kb)
3644
3645
3646 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3643.617 kb on + strand, within Psyr_3045 at 3643.618 kb on - strand, within Psyr_3045 at 3643.618 kb on - strand, within Psyr_3045 at 3643.667 kb on + strand, within Psyr_3045 at 3643.667 kb on + strand, within Psyr_3045 at 3643.667 kb on + strand, within Psyr_3045 at 3643.820 kb on + strand, within Psyr_3045 at 3643.820 kb on + strand, within Psyr_3045 at 3643.973 kb on + strand, within Psyr_3045 at 3643.973 kb on + strand, within Psyr_3045 at 3643.973 kb on + strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3643.974 kb on - strand, within Psyr_3045 at 3644.321 kb on - strand at 3644.486 kb on + strand at 3644.487 kb on - strand at 3644.487 kb on - strand at 3644.536 kb on + strand, within Psyr_3046 at 3644.536 kb on + strand, within Psyr_3046 at 3644.539 kb on + strand, within Psyr_3046 at 3644.539 kb on + strand, within Psyr_3046 at 3644.539 kb on + strand, within Psyr_3046 at 3644.539 kb on + strand, within Psyr_3046 at 3644.540 kb on - strand, within Psyr_3046 at 3644.540 kb on - strand, within Psyr_3046 at 3644.540 kb on - strand, within Psyr_3046 at 3644.860 kb on + strand, within Psyr_3046 at 3644.861 kb on - strand, within Psyr_3046 at 3644.861 kb on - strand, within Psyr_3046 at 3644.861 kb on - strand, within Psyr_3046 at 3645.090 kb on - strand at 3645.090 kb on - strand at 3645.105 kb on + strand at 3645.200 kb on + strand at 3645.219 kb on + strand at 3645.219 kb on + strand at 3645.220 kb on - strand at 3645.223 kb on + strand at 3645.224 kb on - strand at 3645.249 kb on + strand at 3645.249 kb on + strand at 3645.252 kb on + strand at 3645.252 kb on + strand at 3645.393 kb on - strand at 3645.393 kb on - strand at 3645.475 kb on - strand, within Psyr_3047 at 3645.475 kb on - strand, within Psyr_3047 at 3645.475 kb on - strand, within Psyr_3047 at 3645.507 kb on + strand, within Psyr_3047 at 3645.507 kb on + strand, within Psyr_3047 at 3645.507 kb on + strand, within Psyr_3047 at 3645.507 kb on + strand, within Psyr_3047 at 3645.508 kb on - strand, within Psyr_3047 at 3645.508 kb on - strand, within Psyr_3047 at 3645.546 kb on + strand, within Psyr_3047 at 3645.546 kb on + strand, within Psyr_3047 at 3645.546 kb on + strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.547 kb on - strand, within Psyr_3047 at 3645.575 kb on + strand, within Psyr_3047 at 3645.576 kb on - strand, within Psyr_3047 at 3645.576 kb on - strand, within Psyr_3047 at 3645.592 kb on + strand, within Psyr_3047 at 3645.795 kb on + strand at 3645.795 kb on + strand at 3645.795 kb on + strand at 3645.795 kb on + strand at 3645.795 kb on + strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.796 kb on - strand at 3645.817 kb on - strand at 3645.827 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant remove 3,643,617 + Psyr_3045 0.50 +0.8 3,643,618 - Psyr_3045 0.50 -0.0 3,643,618 - Psyr_3045 0.50 -0.5 3,643,667 + Psyr_3045 0.53 +1.0 3,643,667 + Psyr_3045 0.53 +1.9 3,643,667 + Psyr_3045 0.53 -0.1 3,643,820 + Psyr_3045 0.62 +0.8 3,643,820 + Psyr_3045 0.62 +0.4 3,643,973 + Psyr_3045 0.71 +0.5 3,643,973 + Psyr_3045 0.71 -0.7 3,643,973 + Psyr_3045 0.71 -0.9 3,643,974 - Psyr_3045 0.71 -2.1 3,643,974 - Psyr_3045 0.71 -0.5 3,643,974 - Psyr_3045 0.71 +0.2 3,643,974 - Psyr_3045 0.71 -0.1 3,643,974 - Psyr_3045 0.71 -0.7 3,644,321 - +1.5 3,644,486 + +0.3 3,644,487 - -0.7 3,644,487 - -0.2 3,644,536 + Psyr_3046 0.12 -0.9 3,644,536 + Psyr_3046 0.12 -0.9 3,644,539 + Psyr_3046 0.12 -1.2 3,644,539 + Psyr_3046 0.12 +1.3 3,644,539 + Psyr_3046 0.12 -0.1 3,644,539 + Psyr_3046 0.12 +0.1 3,644,540 - Psyr_3046 0.13 -1.3 3,644,540 - Psyr_3046 0.13 -0.6 3,644,540 - Psyr_3046 0.13 +0.5 3,644,860 + Psyr_3046 0.64 -0.5 3,644,861 - Psyr_3046 0.64 -1.2 3,644,861 - Psyr_3046 0.64 -0.9 3,644,861 - Psyr_3046 0.64 -4.2 3,645,090 - -0.3 3,645,090 - -0.3 3,645,105 + +0.2 3,645,200 + -0.7 3,645,219 + +0.3 3,645,219 + -1.4 3,645,220 - -1.0 3,645,223 + -0.0 3,645,224 - +0.1 3,645,249 + +0.1 3,645,249 + +0.2 3,645,252 + +0.1 3,645,252 + -0.8 3,645,393 - -0.3 3,645,393 - -1.0 3,645,475 - Psyr_3047 0.24 -1.0 3,645,475 - Psyr_3047 0.24 +0.8 3,645,475 - Psyr_3047 0.24 -0.1 3,645,507 + Psyr_3047 0.31 -0.8 3,645,507 + Psyr_3047 0.31 +1.1 3,645,507 + Psyr_3047 0.31 +0.8 3,645,507 + Psyr_3047 0.31 +0.1 3,645,508 - Psyr_3047 0.31 -0.7 3,645,508 - Psyr_3047 0.31 -0.4 3,645,546 + Psyr_3047 0.39 +0.3 3,645,546 + Psyr_3047 0.39 -0.6 3,645,546 + Psyr_3047 0.39 -1.6 3,645,547 - Psyr_3047 0.40 +0.0 3,645,547 - Psyr_3047 0.40 -0.7 3,645,547 - Psyr_3047 0.40 +1.0 3,645,547 - Psyr_3047 0.40 +1.5 3,645,547 - Psyr_3047 0.40 +0.2 3,645,547 - Psyr_3047 0.40 +1.1 3,645,575 + Psyr_3047 0.46 -0.2 3,645,576 - Psyr_3047 0.46 -0.3 3,645,576 - Psyr_3047 0.46 -0.1 3,645,592 + Psyr_3047 0.50 -0.0 3,645,795 + +0.9 3,645,795 + +1.5 3,645,795 + +0.2 3,645,795 + -0.3 3,645,795 + -1.6 3,645,796 - +0.9 3,645,796 - -0.3 3,645,796 - +2.0 3,645,796 - -0.4 3,645,796 - -0.4 3,645,796 - -0.5 3,645,796 - -0.1 3,645,796 - -0.8 3,645,817 - -0.1 3,645,827 + -0.0
Or see this region's nucleotide sequence