Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2123

Experiment: Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2121 and Psyr_2122 are separated by 141 nucleotidesPsyr_2122 and Psyr_2123 are separated by 11 nucleotidesPsyr_2123 and Psyr_2124 are separated by 87 nucleotides Psyr_2121: Psyr_2121 - regulatory protein, LysR:LysR, substrate-binding protein, at 2,478,901 to 2,479,821 _2121 Psyr_2122: Psyr_2122 - protocatechuate 3,4-dioxygenase, beta subunit, at 2,479,963 to 2,480,682 _2122 Psyr_2123: Psyr_2123 - protocatechuate 3,4-dioxygenase, alpha subunit, at 2,480,694 to 2,481,296 _2123 Psyr_2124: Psyr_2124 - Benzoate transport, at 2,481,384 to 2,482,733 _2124 Position (kb) 2480 2481 2482Strain fitness (log2 ratio) -2 -1 0 1 2at 2479.938 kb on - strandat 2479.938 kb on - strandat 2480.035 kb on + strand, within Psyr_2122at 2480.035 kb on + strand, within Psyr_2122at 2480.035 kb on + strand, within Psyr_2122at 2480.197 kb on - strand, within Psyr_2122at 2480.197 kb on - strand, within Psyr_2122at 2480.228 kb on - strand, within Psyr_2122at 2480.308 kb on + strand, within Psyr_2122at 2480.309 kb on - strand, within Psyr_2122at 2480.375 kb on - strand, within Psyr_2122at 2480.375 kb on - strand, within Psyr_2122at 2480.405 kb on - strand, within Psyr_2122at 2480.500 kb on + strand, within Psyr_2122at 2480.500 kb on + strand, within Psyr_2122at 2480.895 kb on + strand, within Psyr_2123at 2481.101 kb on + strand, within Psyr_2123at 2481.102 kb on - strand, within Psyr_2123at 2481.102 kb on - strand, within Psyr_2123at 2481.102 kb on - strand, within Psyr_2123at 2481.123 kb on + strand, within Psyr_2123at 2481.123 kb on + strand, within Psyr_2123at 2481.123 kb on + strand, within Psyr_2123at 2481.123 kb on + strand, within Psyr_2123at 2481.124 kb on - strand, within Psyr_2123at 2481.222 kb on + strand, within Psyr_2123at 2481.222 kb on + strand, within Psyr_2123at 2481.223 kb on - strand, within Psyr_2123at 2481.223 kb on - strand, within Psyr_2123at 2481.363 kb on + strandat 2481.363 kb on + strandat 2481.369 kb on + strandat 2481.416 kb on + strandat 2481.416 kb on + strandat 2481.417 kb on - strandat 2481.703 kb on + strand, within Psyr_2124at 2481.704 kb on - strand, within Psyr_2124at 2481.723 kb on + strand, within Psyr_2124at 2481.723 kb on + strand, within Psyr_2124at 2481.724 kb on - strand, within Psyr_2124at 2481.724 kb on - strand, within Psyr_2124at 2481.724 kb on - strand, within Psyr_2124at 2481.820 kb on - strand, within Psyr_2124at 2481.820 kb on - strand, within Psyr_2124at 2482.102 kb on + strand, within Psyr_2124at 2482.167 kb on + strand, within Psyr_2124at 2482.167 kb on + strand, within Psyr_2124at 2482.168 kb on - strand, within Psyr_2124at 2482.200 kb on + strand, within Psyr_2124at 2482.200 kb on + strand, within Psyr_2124at 2482.200 kb on + strand, within Psyr_2124at 2482.200 kb on + strand, within Psyr_2124at 2482.201 kb on - strand, within Psyr_2124

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Per-strain Table

Position Strand Gene LocusTag Fraction Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant
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2,479,938 - +1.3
2,479,938 - +0.4
2,480,035 + Psyr_2122 0.10 +0.1
2,480,035 + Psyr_2122 0.10 -0.4
2,480,035 + Psyr_2122 0.10 -1.7
2,480,197 - Psyr_2122 0.33 +0.0
2,480,197 - Psyr_2122 0.33 +0.6
2,480,228 - Psyr_2122 0.37 -1.0
2,480,308 + Psyr_2122 0.48 -1.2
2,480,309 - Psyr_2122 0.48 -0.2
2,480,375 - Psyr_2122 0.57 -0.2
2,480,375 - Psyr_2122 0.57 +0.0
2,480,405 - Psyr_2122 0.61 +0.5
2,480,500 + Psyr_2122 0.75 +2.0
2,480,500 + Psyr_2122 0.75 -0.6
2,480,895 + Psyr_2123 0.33 +0.7
2,481,101 + Psyr_2123 0.67 +1.1
2,481,102 - Psyr_2123 0.68 +0.1
2,481,102 - Psyr_2123 0.68 -1.0
2,481,102 - Psyr_2123 0.68 +1.4
2,481,123 + Psyr_2123 0.71 -0.0
2,481,123 + Psyr_2123 0.71 +0.3
2,481,123 + Psyr_2123 0.71 +0.6
2,481,123 + Psyr_2123 0.71 -2.2
2,481,124 - Psyr_2123 0.71 +0.3
2,481,222 + Psyr_2123 0.88 +0.3
2,481,222 + Psyr_2123 0.88 -0.5
2,481,223 - Psyr_2123 0.88 +1.6
2,481,223 - Psyr_2123 0.88 +1.1
2,481,363 + -0.3
2,481,363 + -1.8
2,481,369 + -1.0
2,481,416 + +1.1
2,481,416 + +1.2
2,481,417 - +0.8
2,481,703 + Psyr_2124 0.24 -0.3
2,481,704 - Psyr_2124 0.24 +0.4
2,481,723 + Psyr_2124 0.25 +0.1
2,481,723 + Psyr_2124 0.25 -0.6
2,481,724 - Psyr_2124 0.25 +0.7
2,481,724 - Psyr_2124 0.25 -0.9
2,481,724 - Psyr_2124 0.25 +0.4
2,481,820 - Psyr_2124 0.32 +0.5
2,481,820 - Psyr_2124 0.32 +0.4
2,482,102 + Psyr_2124 0.53 -0.3
2,482,167 + Psyr_2124 0.58 -0.5
2,482,167 + Psyr_2124 0.58 +1.4
2,482,168 - Psyr_2124 0.58 +0.2
2,482,200 + Psyr_2124 0.60 +0.3
2,482,200 + Psyr_2124 0.60 -0.0
2,482,200 + Psyr_2124 0.60 -0.2
2,482,200 + Psyr_2124 0.60 -0.1
2,482,201 - Psyr_2124 0.61 +0.2

Or see this region's nucleotide sequence