Experiment: Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1979 and Psyr_1980 are separated by 129 nucleotides Psyr_1980 and Psyr_1981 are separated by 67 nucleotides Psyr_1981 and Psyr_1982 are separated by 63 nucleotides
Psyr_1979: Psyr_1979 - tRNA-U20a,U20b-dihydrouridine synthase, at 2,297,844 to 2,298,809
_1979
Psyr_1980: Psyr_1980 - Heat shock protein Hsp20, at 2,298,939 to 2,299,382
_1980
Psyr_1981: Psyr_1981 - PAS:GGDEF, at 2,299,450 to 2,301,642
_1981
Psyr_1982: Psyr_1982 - transcriptional regulator, LysR family, at 2,301,706 to 2,302,578
_1982
Position (kb)
2299
2300
2301
2302 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2298.731 kb on + strand at 2298.731 kb on + strand at 2298.731 kb on + strand at 2298.732 kb on - strand at 2298.732 kb on - strand at 2298.769 kb on + strand at 2298.770 kb on - strand at 2298.770 kb on - strand at 2298.870 kb on + strand at 2298.871 kb on - strand at 2298.908 kb on - strand at 2298.944 kb on + strand at 2298.945 kb on - strand at 2299.053 kb on + strand, within Psyr_1980 at 2299.053 kb on + strand, within Psyr_1980 at 2299.054 kb on - strand, within Psyr_1980 at 2299.200 kb on + strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.201 kb on - strand, within Psyr_1980 at 2299.259 kb on + strand, within Psyr_1980 at 2299.413 kb on + strand at 2299.414 kb on - strand at 2299.538 kb on + strand at 2299.547 kb on + strand at 2299.547 kb on + strand at 2299.547 kb on - strand at 2299.548 kb on - strand at 2299.548 kb on - strand at 2299.548 kb on - strand at 2299.553 kb on + strand at 2299.554 kb on - strand at 2299.554 kb on - strand at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.742 kb on + strand, within Psyr_1981 at 2299.743 kb on - strand, within Psyr_1981 at 2299.743 kb on - strand, within Psyr_1981 at 2299.851 kb on + strand, within Psyr_1981 at 2299.852 kb on - strand, within Psyr_1981 at 2299.960 kb on - strand, within Psyr_1981 at 2300.043 kb on + strand, within Psyr_1981 at 2300.043 kb on + strand, within Psyr_1981 at 2300.044 kb on - strand, within Psyr_1981 at 2300.083 kb on + strand, within Psyr_1981 at 2300.083 kb on + strand, within Psyr_1981 at 2300.083 kb on + strand, within Psyr_1981 at 2300.084 kb on - strand, within Psyr_1981 at 2300.084 kb on - strand, within Psyr_1981 at 2300.084 kb on - strand, within Psyr_1981 at 2300.117 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.162 kb on + strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.163 kb on - strand, within Psyr_1981 at 2300.527 kb on + strand, within Psyr_1981 at 2300.884 kb on + strand, within Psyr_1981 at 2300.884 kb on + strand, within Psyr_1981 at 2300.886 kb on + strand, within Psyr_1981 at 2300.887 kb on - strand, within Psyr_1981 at 2300.887 kb on - strand, within Psyr_1981 at 2301.021 kb on + strand, within Psyr_1981 at 2301.021 kb on + strand, within Psyr_1981 at 2301.021 kb on + strand, within Psyr_1981 at 2301.086 kb on + strand, within Psyr_1981 at 2301.305 kb on + strand, within Psyr_1981 at 2301.350 kb on + strand, within Psyr_1981 at 2301.351 kb on - strand, within Psyr_1981 at 2301.351 kb on - strand, within Psyr_1981 at 2301.704 kb on - strand at 2301.782 kb on + strand at 2301.783 kb on - strand at 2301.783 kb on - strand at 2301.787 kb on + strand at 2301.788 kb on - strand at 2301.788 kb on - strand at 2301.788 kb on - strand at 2301.805 kb on + strand, within Psyr_1982 at 2301.906 kb on + strand, within Psyr_1982 at 2301.906 kb on + strand, within Psyr_1982 at 2301.906 kb on + strand, within Psyr_1982 at 2301.907 kb on - strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.908 kb on + strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.909 kb on - strand, within Psyr_1982 at 2301.979 kb on - strand, within Psyr_1982 at 2302.021 kb on - strand, within Psyr_1982 at 2302.140 kb on + strand, within Psyr_1982 at 2302.140 kb on + strand, within Psyr_1982 at 2302.141 kb on - strand, within Psyr_1982 at 2302.252 kb on + strand, within Psyr_1982 at 2302.252 kb on + strand, within Psyr_1982 at 2302.373 kb on + strand, within Psyr_1982 at 2302.374 kb on - strand, within Psyr_1982 at 2302.638 kb on + strand at 2302.639 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant remove 2,298,731 + +0.4 2,298,731 + -0.8 2,298,731 + +0.6 2,298,732 - +1.9 2,298,732 - -0.5 2,298,769 + +0.5 2,298,770 - +0.5 2,298,770 - -0.6 2,298,870 + +0.8 2,298,871 - +0.3 2,298,908 - +1.4 2,298,944 + -0.6 2,298,945 - -1.2 2,299,053 + Psyr_1980 0.26 -1.5 2,299,053 + Psyr_1980 0.26 +1.7 2,299,054 - Psyr_1980 0.26 -0.2 2,299,200 + Psyr_1980 0.59 -0.5 2,299,201 - Psyr_1980 0.59 +0.9 2,299,201 - Psyr_1980 0.59 +0.2 2,299,201 - Psyr_1980 0.59 +0.8 2,299,259 + Psyr_1980 0.72 -2.3 2,299,413 + -0.7 2,299,414 - -0.6 2,299,538 + +1.9 2,299,547 + -0.2 2,299,547 + -2.2 2,299,547 - +0.4 2,299,548 - +0.6 2,299,548 - -1.8 2,299,548 - +0.1 2,299,553 + +0.2 2,299,554 - +0.3 2,299,554 - -1.2 2,299,742 + Psyr_1981 0.13 +0.7 2,299,742 + Psyr_1981 0.13 +1.1 2,299,742 + Psyr_1981 0.13 +1.0 2,299,742 + Psyr_1981 0.13 +0.7 2,299,742 + Psyr_1981 0.13 +0.3 2,299,743 - Psyr_1981 0.13 +0.3 2,299,743 - Psyr_1981 0.13 +0.8 2,299,851 + Psyr_1981 0.18 -0.2 2,299,852 - Psyr_1981 0.18 +1.4 2,299,960 - Psyr_1981 0.23 +0.6 2,300,043 + Psyr_1981 0.27 +1.5 2,300,043 + Psyr_1981 0.27 +0.8 2,300,044 - Psyr_1981 0.27 +0.1 2,300,083 + Psyr_1981 0.29 +1.5 2,300,083 + Psyr_1981 0.29 -2.3 2,300,083 + Psyr_1981 0.29 +0.7 2,300,084 - Psyr_1981 0.29 +0.7 2,300,084 - Psyr_1981 0.29 +0.4 2,300,084 - Psyr_1981 0.29 +1.0 2,300,117 + Psyr_1981 0.30 +0.1 2,300,162 + Psyr_1981 0.32 +0.4 2,300,162 + Psyr_1981 0.32 +0.6 2,300,162 + Psyr_1981 0.32 -3.9 2,300,162 + Psyr_1981 0.32 -1.1 2,300,162 + Psyr_1981 0.32 -1.8 2,300,163 - Psyr_1981 0.33 +0.7 2,300,163 - Psyr_1981 0.33 +0.6 2,300,163 - Psyr_1981 0.33 +0.7 2,300,163 - Psyr_1981 0.33 -1.0 2,300,163 - Psyr_1981 0.33 +0.6 2,300,163 - Psyr_1981 0.33 +1.2 2,300,527 + Psyr_1981 0.49 +1.0 2,300,884 + Psyr_1981 0.65 -0.6 2,300,884 + Psyr_1981 0.65 +0.1 2,300,886 + Psyr_1981 0.65 -0.6 2,300,887 - Psyr_1981 0.66 -0.3 2,300,887 - Psyr_1981 0.66 +0.9 2,301,021 + Psyr_1981 0.72 +0.3 2,301,021 + Psyr_1981 0.72 -0.6 2,301,021 + Psyr_1981 0.72 +0.7 2,301,086 + Psyr_1981 0.75 +0.8 2,301,305 + Psyr_1981 0.85 -1.0 2,301,350 + Psyr_1981 0.87 -0.8 2,301,351 - Psyr_1981 0.87 +0.3 2,301,351 - Psyr_1981 0.87 +0.4 2,301,704 - +0.3 2,301,782 + -0.6 2,301,783 - +0.2 2,301,783 - +0.0 2,301,787 + -0.0 2,301,788 - +1.6 2,301,788 - +0.5 2,301,788 - -0.8 2,301,805 + Psyr_1982 0.11 -1.2 2,301,906 + Psyr_1982 0.23 +0.6 2,301,906 + Psyr_1982 0.23 -0.2 2,301,906 + Psyr_1982 0.23 -0.5 2,301,907 - Psyr_1982 0.23 -1.6 2,301,908 + Psyr_1982 0.23 +0.7 2,301,908 + Psyr_1982 0.23 -1.5 2,301,908 + Psyr_1982 0.23 +1.2 2,301,908 + Psyr_1982 0.23 -0.8 2,301,908 + Psyr_1982 0.23 -0.6 2,301,908 + Psyr_1982 0.23 -0.1 2,301,908 + Psyr_1982 0.23 +0.3 2,301,908 + Psyr_1982 0.23 -0.3 2,301,908 + Psyr_1982 0.23 -0.1 2,301,908 + Psyr_1982 0.23 -0.0 2,301,908 + Psyr_1982 0.23 -0.9 2,301,909 - Psyr_1982 0.23 -0.1 2,301,909 - Psyr_1982 0.23 -0.5 2,301,909 - Psyr_1982 0.23 +0.1 2,301,909 - Psyr_1982 0.23 -1.0 2,301,909 - Psyr_1982 0.23 +0.3 2,301,909 - Psyr_1982 0.23 +0.3 2,301,909 - Psyr_1982 0.23 +0.3 2,301,909 - Psyr_1982 0.23 -0.8 2,301,909 - Psyr_1982 0.23 +1.1 2,301,909 - Psyr_1982 0.23 +0.9 2,301,909 - Psyr_1982 0.23 -0.9 2,301,909 - Psyr_1982 0.23 +1.4 2,301,909 - Psyr_1982 0.23 +0.0 2,301,909 - Psyr_1982 0.23 +0.4 2,301,909 - Psyr_1982 0.23 -0.8 2,301,909 - Psyr_1982 0.23 +1.7 2,301,979 - Psyr_1982 0.31 -0.1 2,302,021 - Psyr_1982 0.36 -1.0 2,302,140 + Psyr_1982 0.50 -0.7 2,302,140 + Psyr_1982 0.50 +0.3 2,302,141 - Psyr_1982 0.50 -0.4 2,302,252 + Psyr_1982 0.63 -0.0 2,302,252 + Psyr_1982 0.63 +0.0 2,302,373 + Psyr_1982 0.76 -0.1 2,302,374 - Psyr_1982 0.77 +0.4 2,302,638 + +0.1 2,302,639 - +0.7
Or see this region's nucleotide sequence