Experiment: Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1469 and Psyr_1470 are separated by 296 nucleotides Psyr_1470 and Psyr_1471 are separated by 19 nucleotides Psyr_1471 and Psyr_1472 are separated by 73 nucleotides
Psyr_1469: Psyr_1469 - hypothetical protein, at 1,656,084 to 1,656,401
_1469
Psyr_1470: Psyr_1470 - hypothetical protein, at 1,656,698 to 1,657,270
_1470
Psyr_1471: Psyr_1471 - hypothetical protein, at 1,657,290 to 1,657,826
_1471
Psyr_1472: Psyr_1472 - hypothetical protein, at 1,657,900 to 1,658,265
_1472
Position (kb)
1657
1658 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1656.320 kb on - strand, within Psyr_1469 at 1656.320 kb on - strand, within Psyr_1469 at 1656.320 kb on - strand, within Psyr_1469 at 1656.323 kb on + strand, within Psyr_1469 at 1656.323 kb on + strand, within Psyr_1469 at 1656.326 kb on + strand, within Psyr_1469 at 1656.378 kb on + strand at 1656.379 kb on - strand at 1656.383 kb on + strand at 1656.383 kb on + strand at 1656.386 kb on + strand at 1656.389 kb on + strand at 1656.390 kb on - strand at 1656.414 kb on - strand at 1656.415 kb on + strand at 1656.416 kb on - strand at 1656.416 kb on - strand at 1656.459 kb on - strand at 1656.467 kb on + strand at 1656.468 kb on - strand at 1656.468 kb on - strand at 1656.490 kb on - strand at 1656.490 kb on - strand at 1656.544 kb on + strand at 1656.597 kb on + strand at 1656.640 kb on + strand at 1656.641 kb on - strand at 1656.687 kb on + strand at 1656.697 kb on - strand at 1656.754 kb on + strand at 1656.763 kb on + strand, within Psyr_1470 at 1656.894 kb on + strand, within Psyr_1470 at 1656.894 kb on + strand, within Psyr_1470 at 1656.894 kb on + strand, within Psyr_1470 at 1656.894 kb on + strand, within Psyr_1470 at 1656.894 kb on + strand, within Psyr_1470 at 1656.894 kb on + strand, within Psyr_1470 at 1656.894 kb on + strand, within Psyr_1470 at 1656.895 kb on - strand, within Psyr_1470 at 1656.895 kb on - strand, within Psyr_1470 at 1656.895 kb on - strand, within Psyr_1470 at 1656.895 kb on - strand, within Psyr_1470 at 1656.895 kb on - strand, within Psyr_1470 at 1656.895 kb on - strand, within Psyr_1470 at 1656.895 kb on - strand, within Psyr_1470 at 1656.924 kb on + strand, within Psyr_1470 at 1656.924 kb on + strand, within Psyr_1470 at 1656.925 kb on - strand, within Psyr_1470 at 1656.967 kb on + strand, within Psyr_1470 at 1656.967 kb on + strand, within Psyr_1470 at 1656.968 kb on - strand, within Psyr_1470 at 1656.981 kb on + strand, within Psyr_1470 at 1656.982 kb on - strand, within Psyr_1470 at 1656.982 kb on - strand, within Psyr_1470 at 1656.982 kb on - strand, within Psyr_1470 at 1656.982 kb on - strand, within Psyr_1470 at 1656.982 kb on - strand, within Psyr_1470 at 1656.982 kb on - strand, within Psyr_1470 at 1656.982 kb on - strand, within Psyr_1470 at 1656.982 kb on - strand, within Psyr_1470 at 1656.986 kb on - strand, within Psyr_1470 at 1657.069 kb on + strand, within Psyr_1470 at 1657.070 kb on - strand, within Psyr_1470 at 1657.110 kb on + strand, within Psyr_1470 at 1657.110 kb on + strand, within Psyr_1470 at 1657.110 kb on + strand, within Psyr_1470 at 1657.110 kb on + strand, within Psyr_1470 at 1657.110 kb on + strand, within Psyr_1470 at 1657.111 kb on - strand, within Psyr_1470 at 1657.111 kb on - strand, within Psyr_1470 at 1657.111 kb on - strand, within Psyr_1470 at 1657.111 kb on - strand, within Psyr_1470 at 1657.116 kb on + strand, within Psyr_1470 at 1657.116 kb on + strand, within Psyr_1470 at 1657.117 kb on - strand, within Psyr_1470 at 1657.117 kb on - strand, within Psyr_1470 at 1657.151 kb on + strand, within Psyr_1470 at 1657.151 kb on + strand, within Psyr_1470 at 1657.152 kb on - strand, within Psyr_1470 at 1657.152 kb on - strand, within Psyr_1470 at 1657.152 kb on - strand, within Psyr_1470 at 1657.152 kb on - strand, within Psyr_1470 at 1657.152 kb on - strand, within Psyr_1470 at 1657.180 kb on + strand, within Psyr_1470 at 1657.180 kb on + strand, within Psyr_1470 at 1657.242 kb on + strand at 1657.243 kb on - strand at 1657.243 kb on - strand at 1657.243 kb on - strand at 1657.258 kb on + strand at 1657.275 kb on + strand at 1657.275 kb on + strand at 1657.275 kb on + strand at 1657.275 kb on + strand at 1657.275 kb on + strand at 1657.275 kb on + strand at 1657.276 kb on - strand at 1657.276 kb on - strand at 1657.276 kb on - strand at 1657.276 kb on - strand at 1657.276 kb on - strand at 1657.276 kb on - strand at 1657.276 kb on - strand at 1657.276 kb on - strand at 1657.306 kb on + strand at 1657.306 kb on + strand at 1657.306 kb on + strand at 1657.306 kb on + strand at 1657.307 kb on - strand at 1657.307 kb on - strand at 1657.307 kb on - strand at 1657.307 kb on - strand at 1657.328 kb on + strand at 1657.329 kb on - strand at 1657.390 kb on + strand, within Psyr_1471 at 1657.391 kb on - strand, within Psyr_1471 at 1657.396 kb on + strand, within Psyr_1471 at 1657.404 kb on - strand, within Psyr_1471 at 1657.586 kb on - strand, within Psyr_1471 at 1657.629 kb on + strand, within Psyr_1471 at 1657.683 kb on - strand, within Psyr_1471 at 1657.683 kb on - strand, within Psyr_1471 at 1657.754 kb on + strand, within Psyr_1471 at 1657.772 kb on + strand, within Psyr_1471 at 1657.773 kb on - strand at 1657.804 kb on + strand at 1657.824 kb on + strand at 1657.824 kb on + strand at 1657.824 kb on + strand at 1657.825 kb on - strand at 1657.825 kb on - strand at 1657.825 kb on - strand at 1657.882 kb on + strand at 1657.899 kb on - strand at 1657.899 kb on - strand at 1657.941 kb on + strand, within Psyr_1472 at 1657.941 kb on + strand, within Psyr_1472 at 1658.052 kb on - strand, within Psyr_1472 at 1658.190 kb on + strand, within Psyr_1472 at 1658.190 kb on + strand, within Psyr_1472 at 1658.190 kb on + strand, within Psyr_1472 at 1658.190 kb on + strand, within Psyr_1472 at 1658.190 kb on + strand, within Psyr_1472 at 1658.190 kb on + strand, within Psyr_1472 at 1658.190 kb on + strand, within Psyr_1472 at 1658.191 kb on - strand, within Psyr_1472 at 1658.191 kb on - strand at 1658.191 kb on - strand, within Psyr_1472 at 1658.191 kb on - strand, within Psyr_1472 at 1658.191 kb on - strand, within Psyr_1472 at 1658.191 kb on - strand, within Psyr_1472 at 1658.191 kb on - strand, within Psyr_1472 at 1658.191 kb on - strand, within Psyr_1472 at 1658.191 kb on - strand, within Psyr_1472 at 1658.191 kb on - strand, within Psyr_1472 at 1658.191 kb on - strand, within Psyr_1472 at 1658.226 kb on + strand, within Psyr_1472 at 1658.226 kb on + strand, within Psyr_1472 at 1658.260 kb on - strand at 1658.342 kb on - strand at 1658.342 kb on - strand at 1658.434 kb on - strand at 1658.482 kb on + strand at 1658.483 kb on - strand at 1658.578 kb on + strand at 1658.586 kb on + strand at 1658.586 kb on + strand at 1658.586 kb on + strand at 1658.587 kb on - strand at 1658.587 kb on - strand at 1658.702 kb on + strand at 1658.702 kb on + strand at 1658.702 kb on + strand at 1658.702 kb on + strand at 1658.703 kb on - strand at 1658.703 kb on - strand at 1658.703 kb on - strand at 1658.703 kb on - strand at 1658.727 kb on - strand at 1658.745 kb on - strand at 1658.768 kb on + strand at 1658.769 kb on - strand at 1658.783 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant remove 1,656,320 - Psyr_1469 0.74 +0.4 1,656,320 - Psyr_1469 0.74 +0.9 1,656,320 - Psyr_1469 0.74 +0.1 1,656,323 + Psyr_1469 0.75 -0.3 1,656,323 + Psyr_1469 0.75 +0.8 1,656,326 + Psyr_1469 0.76 +1.2 1,656,378 + +2.3 1,656,379 - -0.3 1,656,383 + +1.3 1,656,383 + +1.3 1,656,386 + -0.2 1,656,389 + -2.0 1,656,390 - +0.4 1,656,414 - +0.3 1,656,415 + +0.4 1,656,416 - +0.7 1,656,416 - +1.6 1,656,459 - -0.1 1,656,467 + +0.7 1,656,468 - +0.4 1,656,468 - -0.4 1,656,490 - +0.2 1,656,490 - -1.1 1,656,544 + -0.9 1,656,597 + -0.1 1,656,640 + +0.6 1,656,641 - -0.5 1,656,687 + -0.1 1,656,697 - +0.1 1,656,754 + -2.0 1,656,763 + Psyr_1470 0.11 +0.5 1,656,894 + Psyr_1470 0.34 -0.1 1,656,894 + Psyr_1470 0.34 -0.1 1,656,894 + Psyr_1470 0.34 -1.6 1,656,894 + Psyr_1470 0.34 +0.6 1,656,894 + Psyr_1470 0.34 -1.0 1,656,894 + Psyr_1470 0.34 +0.8 1,656,894 + Psyr_1470 0.34 -0.6 1,656,895 - Psyr_1470 0.34 +0.6 1,656,895 - Psyr_1470 0.34 +0.4 1,656,895 - Psyr_1470 0.34 -0.1 1,656,895 - Psyr_1470 0.34 -0.6 1,656,895 - Psyr_1470 0.34 -1.4 1,656,895 - Psyr_1470 0.34 -3.1 1,656,895 - Psyr_1470 0.34 +0.5 1,656,924 + Psyr_1470 0.39 +0.5 1,656,924 + Psyr_1470 0.39 +0.3 1,656,925 - Psyr_1470 0.40 +0.3 1,656,967 + Psyr_1470 0.47 +1.1 1,656,967 + Psyr_1470 0.47 -0.3 1,656,968 - Psyr_1470 0.47 -1.9 1,656,981 + Psyr_1470 0.49 -0.2 1,656,982 - Psyr_1470 0.50 +1.3 1,656,982 - Psyr_1470 0.50 -0.4 1,656,982 - Psyr_1470 0.50 +0.1 1,656,982 - Psyr_1470 0.50 -0.6 1,656,982 - Psyr_1470 0.50 +1.3 1,656,982 - Psyr_1470 0.50 +0.2 1,656,982 - Psyr_1470 0.50 +0.7 1,656,982 - Psyr_1470 0.50 -1.3 1,656,986 - Psyr_1470 0.50 -1.1 1,657,069 + Psyr_1470 0.65 -1.1 1,657,070 - Psyr_1470 0.65 +0.0 1,657,110 + Psyr_1470 0.72 +2.4 1,657,110 + Psyr_1470 0.72 -0.8 1,657,110 + Psyr_1470 0.72 +0.9 1,657,110 + Psyr_1470 0.72 +0.1 1,657,110 + Psyr_1470 0.72 +1.4 1,657,111 - Psyr_1470 0.72 -0.9 1,657,111 - Psyr_1470 0.72 -0.5 1,657,111 - Psyr_1470 0.72 +0.1 1,657,111 - Psyr_1470 0.72 +2.4 1,657,116 + Psyr_1470 0.73 -0.2 1,657,116 + Psyr_1470 0.73 +0.5 1,657,117 - Psyr_1470 0.73 +0.5 1,657,117 - Psyr_1470 0.73 +0.6 1,657,151 + Psyr_1470 0.79 -1.9 1,657,151 + Psyr_1470 0.79 +2.2 1,657,152 - Psyr_1470 0.79 -0.6 1,657,152 - Psyr_1470 0.79 -0.5 1,657,152 - Psyr_1470 0.79 +1.0 1,657,152 - Psyr_1470 0.79 -0.1 1,657,152 - Psyr_1470 0.79 +0.0 1,657,180 + Psyr_1470 0.84 -0.8 1,657,180 + Psyr_1470 0.84 -1.5 1,657,242 + -1.6 1,657,243 - -1.2 1,657,243 - -0.4 1,657,243 - -1.0 1,657,258 + +0.8 1,657,275 + +0.8 1,657,275 + -0.3 1,657,275 + -0.8 1,657,275 + -0.1 1,657,275 + +0.4 1,657,275 + +0.4 1,657,276 - -0.5 1,657,276 - +2.2 1,657,276 - +0.1 1,657,276 - +0.9 1,657,276 - +0.5 1,657,276 - +0.4 1,657,276 - -1.1 1,657,276 - -0.3 1,657,306 + -1.8 1,657,306 + -0.3 1,657,306 + -0.5 1,657,306 + +1.5 1,657,307 - -0.4 1,657,307 - -0.4 1,657,307 - -2.7 1,657,307 - +1.4 1,657,328 + +0.7 1,657,329 - +1.6 1,657,390 + Psyr_1471 0.19 -0.6 1,657,391 - Psyr_1471 0.19 -0.5 1,657,396 + Psyr_1471 0.20 -0.8 1,657,404 - Psyr_1471 0.21 +0.6 1,657,586 - Psyr_1471 0.55 +0.2 1,657,629 + Psyr_1471 0.63 -0.6 1,657,683 - Psyr_1471 0.73 -1.7 1,657,683 - Psyr_1471 0.73 +0.2 1,657,754 + Psyr_1471 0.86 +0.1 1,657,772 + Psyr_1471 0.90 -1.0 1,657,773 - -0.6 1,657,804 + +0.3 1,657,824 + -1.0 1,657,824 + +0.1 1,657,824 + -1.2 1,657,825 - +0.3 1,657,825 - +0.3 1,657,825 - +0.1 1,657,882 + -0.1 1,657,899 - +1.1 1,657,899 - +0.6 1,657,941 + Psyr_1472 0.11 -0.4 1,657,941 + Psyr_1472 0.11 +0.4 1,658,052 - Psyr_1472 0.42 -0.4 1,658,190 + Psyr_1472 0.79 +0.0 1,658,190 + Psyr_1472 0.79 -0.8 1,658,190 + Psyr_1472 0.79 +0.1 1,658,190 + Psyr_1472 0.79 -0.3 1,658,190 + Psyr_1472 0.79 -0.1 1,658,190 + Psyr_1472 0.79 -0.2 1,658,190 + Psyr_1472 0.79 -0.9 1,658,191 - Psyr_1472 0.80 -0.7 1,658,191 - -0.8 1,658,191 - Psyr_1472 0.80 +0.6 1,658,191 - Psyr_1472 0.80 -3.6 1,658,191 - Psyr_1472 0.80 -2.5 1,658,191 - Psyr_1472 0.80 -0.7 1,658,191 - Psyr_1472 0.80 -0.1 1,658,191 - Psyr_1472 0.80 -0.9 1,658,191 - Psyr_1472 0.80 +0.2 1,658,191 - Psyr_1472 0.80 +0.6 1,658,191 - Psyr_1472 0.80 +0.1 1,658,226 + Psyr_1472 0.89 -0.1 1,658,226 + Psyr_1472 0.89 +0.1 1,658,260 - -0.7 1,658,342 - +0.0 1,658,342 - +0.6 1,658,434 - +1.9 1,658,482 + -1.1 1,658,483 - +0.7 1,658,578 + -0.3 1,658,586 + -0.2 1,658,586 + +0.5 1,658,586 + +0.3 1,658,587 - -2.1 1,658,587 - +0.5 1,658,702 + -0.2 1,658,702 + -0.7 1,658,702 + -0.4 1,658,702 + +0.5 1,658,703 - -0.3 1,658,703 - -0.7 1,658,703 - +0.2 1,658,703 - -0.4 1,658,727 - +0.2 1,658,745 - -0.6 1,658,768 + -0.7 1,658,769 - +0.5 1,658,783 - +1.7
Or see this region's nucleotide sequence