Experiment: Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0469 and Psyr_0470 are separated by 75 nucleotides Psyr_0470 and Psyr_0471 are separated by 9 nucleotides Psyr_0471 and Psyr_0472 overlap by 4 nucleotides Psyr_0472 and Psyr_0473 are separated by 33 nucleotides
Psyr_0469: Psyr_0469 - dihydroxyacid dehydratase, at 508,137 to 509,984
_0469
Psyr_0470: Psyr_0470 - conserved hypothetical protein, at 510,060 to 510,383
_0470
Psyr_0471: Psyr_0471 - coproporphyrinogen III oxidase, anaerobic, at 510,393 to 511,607
_0471
Psyr_0472: Psyr_0472 - Ham1-like protein, at 511,604 to 512,197
_0472
Psyr_0473: Psyr_0473 - Methionine biosynthesis MetW, at 512,231 to 512,851
_0473
Position (kb) 510
511
512 Strain fitness (log2 ratio) -2
-1
0
1
2 at 509.404 kb on + strand, within Psyr_0469 at 509.405 kb on - strand, within Psyr_0469 at 509.405 kb on - strand, within Psyr_0469 at 509.405 kb on - strand, within Psyr_0469 at 509.405 kb on - strand, within Psyr_0469 at 509.406 kb on + strand, within Psyr_0469 at 509.406 kb on + strand, within Psyr_0469 at 509.407 kb on - strand, within Psyr_0469 at 509.611 kb on - strand, within Psyr_0469 at 509.623 kb on - strand, within Psyr_0469 at 509.910 kb on + strand at 509.996 kb on - strand at 510.002 kb on + strand at 510.003 kb on - strand at 510.003 kb on - strand at 510.003 kb on - strand at 510.061 kb on + strand at 510.061 kb on + strand at 510.061 kb on + strand at 510.062 kb on - strand at 510.062 kb on - strand at 510.062 kb on - strand at 510.124 kb on + strand, within Psyr_0470 at 510.124 kb on + strand, within Psyr_0470 at 510.124 kb on + strand, within Psyr_0470 at 510.124 kb on + strand, within Psyr_0470 at 510.169 kb on + strand, within Psyr_0470 at 510.169 kb on + strand, within Psyr_0470 at 510.170 kb on - strand, within Psyr_0470 at 510.170 kb on - strand, within Psyr_0470 at 510.394 kb on + strand at 510.394 kb on + strand at 510.394 kb on + strand at 510.394 kb on + strand at 510.394 kb on + strand at 510.394 kb on + strand at 510.395 kb on - strand at 510.395 kb on - strand at 510.406 kb on + strand at 510.406 kb on + strand at 510.407 kb on - strand at 510.550 kb on - strand, within Psyr_0471 at 510.665 kb on - strand, within Psyr_0471 at 510.692 kb on + strand, within Psyr_0471 at 510.772 kb on + strand, within Psyr_0471 at 510.772 kb on + strand, within Psyr_0471 at 510.772 kb on + strand, within Psyr_0471 at 510.772 kb on + strand, within Psyr_0471 at 510.811 kb on + strand, within Psyr_0471 at 510.811 kb on + strand, within Psyr_0471 at 510.812 kb on - strand, within Psyr_0471 at 510.826 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.469 kb on - strand, within Psyr_0471 at 511.496 kb on + strand at 511.496 kb on + strand at 511.497 kb on - strand at 511.504 kb on + strand at 511.504 kb on + strand at 511.504 kb on + strand at 511.504 kb on + strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.835 kb on + strand, within Psyr_0472 at 511.835 kb on + strand, within Psyr_0472 at 511.836 kb on - strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.948 kb on + strand, within Psyr_0472 at 512.277 kb on + strand at 512.277 kb on + strand at 512.303 kb on - strand, within Psyr_0473 at 512.436 kb on + strand, within Psyr_0473 at 512.437 kb on - strand, within Psyr_0473 at 512.443 kb on + strand, within Psyr_0473 at 512.444 kb on - strand, within Psyr_0473 at 512.444 kb on - strand, within Psyr_0473 at 512.445 kb on + strand, within Psyr_0473 at 512.445 kb on + strand, within Psyr_0473 at 512.445 kb on + strand, within Psyr_0473 at 512.446 kb on - strand, within Psyr_0473 at 512.446 kb on - strand, within Psyr_0473 at 512.446 kb on - strand, within Psyr_0473 at 512.490 kb on + strand, within Psyr_0473 at 512.490 kb on + strand, within Psyr_0473 at 512.491 kb on - strand, within Psyr_0473 at 512.491 kb on - strand, within Psyr_0473
Per-strain Table
Position Strand Gene LocusTag Fraction Pseudomonas syringae pv. syringae B728a MLX with Paraburkholderia sabiae T6SS mutant remove 509,404 + Psyr_0469 0.69 +0.5 509,405 - Psyr_0469 0.69 +0.4 509,405 - Psyr_0469 0.69 +0.2 509,405 - Psyr_0469 0.69 -1.5 509,405 - Psyr_0469 0.69 +0.2 509,406 + Psyr_0469 0.69 +1.1 509,406 + Psyr_0469 0.69 -0.1 509,407 - Psyr_0469 0.69 -0.6 509,611 - Psyr_0469 0.80 +0.5 509,623 - Psyr_0469 0.80 +0.6 509,910 + +0.3 509,996 - -1.2 510,002 + +1.1 510,003 - +0.7 510,003 - +0.9 510,003 - -0.8 510,061 + -0.1 510,061 + -0.5 510,061 + +0.1 510,062 - +1.1 510,062 - -0.3 510,062 - -0.3 510,124 + Psyr_0470 0.20 +0.1 510,124 + Psyr_0470 0.20 +1.0 510,124 + Psyr_0470 0.20 +0.6 510,124 + Psyr_0470 0.20 +0.9 510,169 + Psyr_0470 0.34 -0.5 510,169 + Psyr_0470 0.34 -1.7 510,170 - Psyr_0470 0.34 -1.1 510,170 - Psyr_0470 0.34 +0.0 510,394 + +0.4 510,394 + -0.3 510,394 + +0.2 510,394 + +0.2 510,394 + -1.1 510,394 + -0.8 510,395 - +0.4 510,395 - -0.9 510,406 + -1.5 510,406 + -0.7 510,407 - -0.4 510,550 - Psyr_0471 0.13 +0.4 510,665 - Psyr_0471 0.22 +1.2 510,692 + Psyr_0471 0.25 -1.3 510,772 + Psyr_0471 0.31 -1.1 510,772 + Psyr_0471 0.31 -0.3 510,772 + Psyr_0471 0.31 -1.7 510,772 + Psyr_0471 0.31 -0.2 510,811 + Psyr_0471 0.34 -0.7 510,811 + Psyr_0471 0.34 -1.3 510,812 - Psyr_0471 0.34 -0.1 510,826 + Psyr_0471 0.36 -0.3 511,417 + Psyr_0471 0.84 -1.0 511,417 + Psyr_0471 0.84 +0.1 511,417 + Psyr_0471 0.84 -1.9 511,417 + Psyr_0471 0.84 -2.0 511,417 + Psyr_0471 0.84 -1.9 511,417 + Psyr_0471 0.84 +0.2 511,417 + Psyr_0471 0.84 -2.1 511,417 + Psyr_0471 0.84 -2.3 511,417 + Psyr_0471 0.84 +0.6 511,417 + Psyr_0471 0.84 +0.2 511,417 + Psyr_0471 0.84 +0.2 511,418 - Psyr_0471 0.84 +1.4 511,418 - Psyr_0471 0.84 -0.5 511,418 - Psyr_0471 0.84 -0.7 511,418 - Psyr_0471 0.84 -2.3 511,418 - Psyr_0471 0.84 -0.4 511,418 - Psyr_0471 0.84 -0.0 511,418 - Psyr_0471 0.84 -0.7 511,469 - Psyr_0471 0.89 -1.4 511,496 + +0.4 511,496 + -0.0 511,497 - +1.0 511,504 + -0.8 511,504 + +0.4 511,504 + -0.9 511,504 + +0.9 511,505 - -1.6 511,505 - +0.8 511,505 - -0.1 511,505 - -0.1 511,505 - -1.5 511,505 - +2.1 511,835 + Psyr_0472 0.39 -0.5 511,835 + Psyr_0472 0.39 -0.9 511,836 - Psyr_0472 0.39 -1.0 511,944 + Psyr_0472 0.57 +0.8 511,944 + Psyr_0472 0.57 +0.4 511,944 + Psyr_0472 0.57 -0.4 511,944 + Psyr_0472 0.57 -0.1 511,944 + Psyr_0472 0.57 -2.5 511,944 + Psyr_0472 0.57 +0.3 511,944 + Psyr_0472 0.57 +0.5 511,944 + Psyr_0472 0.57 +0.8 511,944 + Psyr_0472 0.57 -0.3 511,944 + Psyr_0472 0.57 +0.3 511,945 - Psyr_0472 0.57 -0.6 511,945 - Psyr_0472 0.57 -0.1 511,945 - Psyr_0472 0.57 +0.1 511,945 - Psyr_0472 0.57 -2.0 511,945 - Psyr_0472 0.57 -0.3 511,945 - Psyr_0472 0.57 +0.1 511,948 + Psyr_0472 0.58 +1.0 512,277 + -0.7 512,277 + -0.1 512,303 - Psyr_0473 0.12 +1.6 512,436 + Psyr_0473 0.33 -0.2 512,437 - Psyr_0473 0.33 +0.4 512,443 + Psyr_0473 0.34 -0.3 512,444 - Psyr_0473 0.34 -0.6 512,444 - Psyr_0473 0.34 -1.0 512,445 + Psyr_0473 0.34 -0.4 512,445 + Psyr_0473 0.34 -0.7 512,445 + Psyr_0473 0.34 -0.7 512,446 - Psyr_0473 0.35 +1.2 512,446 - Psyr_0473 0.35 +0.8 512,446 - Psyr_0473 0.35 +0.1 512,490 + Psyr_0473 0.42 -0.5 512,490 + Psyr_0473 0.42 +2.2 512,491 - Psyr_0473 0.42 +0.3 512,491 - Psyr_0473 0.42 +0.0
Or see this region's nucleotide sequence