Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS20230

Experiment: SMSA no antibiotic control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS20225 and RALBFv3_RS20230 overlap by 4 nucleotidesRALBFv3_RS20230 and RALBFv3_RS20235 are separated by 88 nucleotides RALBFv3_RS20225: RALBFv3_RS20225 - RNA ligase RtcB family protein, at 1,230,986 to 1,232,125 _RS20225 RALBFv3_RS20230: RALBFv3_RS20230 - peptide chain release factor H, at 1,232,122 to 1,232,745 _RS20230 RALBFv3_RS20235: RALBFv3_RS20235 - LysR family transcriptional regulator, at 1,232,834 to 1,233,757 _RS20235 Position (kb) 1232 1233Strain fitness (log2 ratio) -1 0 1at 1231.312 kb on + strand, within RALBFv3_RS20225at 1231.603 kb on + strand, within RALBFv3_RS20225at 1231.817 kb on + strand, within RALBFv3_RS20225at 1231.991 kb on + strand, within RALBFv3_RS20225at 1232.013 kb on - strandat 1232.097 kb on - strandat 1233.038 kb on - strand, within RALBFv3_RS20235at 1233.038 kb on - strand, within RALBFv3_RS20235at 1233.039 kb on + strand, within RALBFv3_RS20235at 1233.039 kb on + strand, within RALBFv3_RS20235at 1233.039 kb on + strand, within RALBFv3_RS20235at 1233.040 kb on - strand, within RALBFv3_RS20235at 1233.040 kb on - strand, within RALBFv3_RS20235at 1233.124 kb on - strand, within RALBFv3_RS20235at 1233.241 kb on - strand, within RALBFv3_RS20235at 1233.241 kb on - strand, within RALBFv3_RS20235at 1233.241 kb on - strand, within RALBFv3_RS20235at 1233.426 kb on + strand, within RALBFv3_RS20235at 1233.426 kb on + strand, within RALBFv3_RS20235

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA no antibiotic control
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1,231,312 + RALBFv3_RS20225 0.29 -0.0
1,231,603 + RALBFv3_RS20225 0.54 -0.9
1,231,817 + RALBFv3_RS20225 0.73 +0.1
1,231,991 + RALBFv3_RS20225 0.88 +0.2
1,232,013 - -0.6
1,232,097 - +0.6
1,233,038 - RALBFv3_RS20235 0.22 -0.2
1,233,038 - RALBFv3_RS20235 0.22 +1.1
1,233,039 + RALBFv3_RS20235 0.22 -0.7
1,233,039 + RALBFv3_RS20235 0.22 -0.1
1,233,039 + RALBFv3_RS20235 0.22 +0.2
1,233,040 - RALBFv3_RS20235 0.22 -0.5
1,233,040 - RALBFv3_RS20235 0.22 +0.2
1,233,124 - RALBFv3_RS20235 0.31 -0.4
1,233,241 - RALBFv3_RS20235 0.44 -0.7
1,233,241 - RALBFv3_RS20235 0.44 -0.5
1,233,241 - RALBFv3_RS20235 0.44 -0.6
1,233,426 + RALBFv3_RS20235 0.64 -0.0
1,233,426 + RALBFv3_RS20235 0.64 +0.1

Or see this region's nucleotide sequence