Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS18215 and RALBFv3_RS18220 are separated by 273 nucleotides RALBFv3_RS18220 and RALBFv3_RS18225 are separated by 100 nucleotides
RALBFv3_RS18215: RALBFv3_RS18215 - acid phosphatase, at 613,389 to 615,545
_RS18215
RALBFv3_RS18220: RALBFv3_RS18220 - cytochrome-c peroxidase, at 615,819 to 617,162
_RS18220
RALBFv3_RS18225: RALBFv3_RS18225 - TerC family protein, at 617,263 to 618,843
_RS18225
Position (kb) 615
616
617
618 Strain fitness (log2 ratio) -2
-1
0
1 at 614.860 kb on + strand, within RALBFv3_RS18215 at 615.320 kb on - strand, within RALBFv3_RS18215 at 615.340 kb on + strand at 615.341 kb on - strand at 615.341 kb on - strand at 615.341 kb on - strand at 615.412 kb on + strand at 615.412 kb on + strand at 615.784 kb on - strand at 615.972 kb on + strand, within RALBFv3_RS18220 at 616.119 kb on + strand, within RALBFv3_RS18220 at 616.204 kb on - strand, within RALBFv3_RS18220 at 616.287 kb on + strand, within RALBFv3_RS18220 at 616.521 kb on + strand, within RALBFv3_RS18220 at 616.522 kb on - strand, within RALBFv3_RS18220 at 616.527 kb on + strand, within RALBFv3_RS18220 at 616.539 kb on + strand, within RALBFv3_RS18220 at 616.540 kb on - strand, within RALBFv3_RS18220 at 616.540 kb on - strand, within RALBFv3_RS18220 at 616.640 kb on + strand, within RALBFv3_RS18220 at 616.909 kb on - strand, within RALBFv3_RS18220 at 617.262 kb on + strand at 617.262 kb on + strand at 617.262 kb on + strand at 617.263 kb on - strand at 617.263 kb on - strand at 617.263 kb on - strand at 617.263 kb on - strand at 617.899 kb on + strand, within RALBFv3_RS18225 at 617.899 kb on + strand, within RALBFv3_RS18225
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 614,860 + RALBFv3_RS18215 0.68 +1.1 615,320 - RALBFv3_RS18215 0.90 -0.5 615,340 + +0.1 615,341 - -0.1 615,341 - -0.1 615,341 - -0.1 615,412 + +0.1 615,412 + -0.0 615,784 - -0.8 615,972 + RALBFv3_RS18220 0.11 -0.1 616,119 + RALBFv3_RS18220 0.22 +1.1 616,204 - RALBFv3_RS18220 0.29 -1.6 616,287 + RALBFv3_RS18220 0.35 -0.1 616,521 + RALBFv3_RS18220 0.52 -0.2 616,522 - RALBFv3_RS18220 0.52 -0.1 616,527 + RALBFv3_RS18220 0.53 -0.9 616,539 + RALBFv3_RS18220 0.54 -0.1 616,540 - RALBFv3_RS18220 0.54 -0.6 616,540 - RALBFv3_RS18220 0.54 -2.1 616,640 + RALBFv3_RS18220 0.61 +0.1 616,909 - RALBFv3_RS18220 0.81 -0.8 617,262 + -2.2 617,262 + -1.2 617,262 + +0.2 617,263 - +0.0 617,263 - +0.8 617,263 - -0.3 617,263 - -1.7 617,899 + RALBFv3_RS18225 0.40 -2.4 617,899 + RALBFv3_RS18225 0.40 -2.7
Or see this region's nucleotide sequence