Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS05800 and RALBFv3_RS05805 are separated by 8 nucleotides RALBFv3_RS05805 and RALBFv3_RS05810 are separated by 61 nucleotides
RALBFv3_RS05800: RALBFv3_RS05800 - two-component sensor histidine kinase, at 1,291,003 to 1,292,331
_RS05800
RALBFv3_RS05805: RALBFv3_RS05805 - hybrid sensor histidine kinase/response regulator, at 1,292,340 to 1,293,458
_RS05805
RALBFv3_RS05810: RALBFv3_RS05810 - response regulator, at 1,293,520 to 1,294,530
_RS05810
Position (kb)
1292
1293
1294 Strain fitness (log2 ratio)
-1
0
1
2 at 1291.378 kb on - strand, within RALBFv3_RS05800 at 1291.404 kb on + strand, within RALBFv3_RS05800 at 1291.404 kb on + strand, within RALBFv3_RS05800 at 1291.404 kb on + strand, within RALBFv3_RS05800 at 1291.405 kb on - strand, within RALBFv3_RS05800 at 1291.408 kb on + strand, within RALBFv3_RS05800 at 1291.408 kb on + strand, within RALBFv3_RS05800 at 1291.408 kb on + strand, within RALBFv3_RS05800 at 1291.409 kb on - strand, within RALBFv3_RS05800 at 1291.409 kb on - strand, within RALBFv3_RS05800 at 1291.409 kb on - strand, within RALBFv3_RS05800 at 1291.409 kb on - strand, within RALBFv3_RS05800 at 1291.409 kb on - strand, within RALBFv3_RS05800 at 1291.470 kb on + strand, within RALBFv3_RS05800 at 1291.489 kb on - strand, within RALBFv3_RS05800 at 1291.519 kb on + strand, within RALBFv3_RS05800 at 1291.520 kb on - strand, within RALBFv3_RS05800 at 1291.520 kb on - strand, within RALBFv3_RS05800 at 1291.520 kb on - strand, within RALBFv3_RS05800 at 1291.521 kb on + strand, within RALBFv3_RS05800 at 1291.522 kb on - strand, within RALBFv3_RS05800 at 1291.522 kb on - strand, within RALBFv3_RS05800 at 1291.522 kb on - strand, within RALBFv3_RS05800 at 1291.525 kb on + strand, within RALBFv3_RS05800 at 1291.749 kb on + strand, within RALBFv3_RS05800 at 1291.750 kb on - strand, within RALBFv3_RS05800 at 1292.236 kb on + strand at 1292.329 kb on + strand at 1292.330 kb on - strand at 1292.376 kb on + strand at 1292.377 kb on - strand at 1292.433 kb on + strand at 1292.434 kb on - strand at 1293.045 kb on + strand, within RALBFv3_RS05805 at 1293.046 kb on - strand, within RALBFv3_RS05805 at 1293.249 kb on + strand, within RALBFv3_RS05805 at 1293.249 kb on + strand, within RALBFv3_RS05805 at 1293.251 kb on + strand, within RALBFv3_RS05805 at 1293.251 kb on + strand, within RALBFv3_RS05805 at 1293.469 kb on - strand at 1293.479 kb on - strand at 1293.479 kb on - strand at 1293.479 kb on - strand at 1293.506 kb on - strand at 1293.556 kb on + strand at 1293.556 kb on + strand at 1293.556 kb on + strand at 1293.556 kb on + strand at 1293.556 kb on + strand at 1293.557 kb on - strand at 1293.586 kb on + strand at 1293.587 kb on - strand at 1293.587 kb on - strand at 1293.587 kb on - strand at 1293.587 kb on - strand at 1293.787 kb on + strand, within RALBFv3_RS05810 at 1293.841 kb on + strand, within RALBFv3_RS05810 at 1293.841 kb on + strand, within RALBFv3_RS05810 at 1294.003 kb on + strand, within RALBFv3_RS05810 at 1294.003 kb on + strand, within RALBFv3_RS05810 at 1294.049 kb on - strand, within RALBFv3_RS05810
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 1,291,378 - RALBFv3_RS05800 0.28 +1.2 1,291,404 + RALBFv3_RS05800 0.30 -0.8 1,291,404 + RALBFv3_RS05800 0.30 -0.3 1,291,404 + RALBFv3_RS05800 0.30 +0.6 1,291,405 - RALBFv3_RS05800 0.30 +1.4 1,291,408 + RALBFv3_RS05800 0.30 -0.8 1,291,408 + RALBFv3_RS05800 0.30 +1.7 1,291,408 + RALBFv3_RS05800 0.30 +1.7 1,291,409 - RALBFv3_RS05800 0.31 +1.8 1,291,409 - RALBFv3_RS05800 0.31 +1.0 1,291,409 - RALBFv3_RS05800 0.31 +1.2 1,291,409 - RALBFv3_RS05800 0.31 +1.2 1,291,409 - RALBFv3_RS05800 0.31 +1.2 1,291,470 + RALBFv3_RS05800 0.35 +2.0 1,291,489 - RALBFv3_RS05800 0.37 +1.1 1,291,519 + RALBFv3_RS05800 0.39 +0.2 1,291,520 - RALBFv3_RS05800 0.39 +1.1 1,291,520 - RALBFv3_RS05800 0.39 +1.5 1,291,520 - RALBFv3_RS05800 0.39 +1.3 1,291,521 + RALBFv3_RS05800 0.39 -0.8 1,291,522 - RALBFv3_RS05800 0.39 +1.4 1,291,522 - RALBFv3_RS05800 0.39 +1.4 1,291,522 - RALBFv3_RS05800 0.39 -1.1 1,291,525 + RALBFv3_RS05800 0.39 -1.2 1,291,749 + RALBFv3_RS05800 0.56 -0.5 1,291,750 - RALBFv3_RS05800 0.56 +1.2 1,292,236 + +1.4 1,292,329 + -0.2 1,292,330 - +0.7 1,292,376 + +1.8 1,292,377 - +1.3 1,292,433 + +0.9 1,292,434 - +1.4 1,293,045 + RALBFv3_RS05805 0.63 +1.0 1,293,046 - RALBFv3_RS05805 0.63 +1.1 1,293,249 + RALBFv3_RS05805 0.81 +1.5 1,293,249 + RALBFv3_RS05805 0.81 +0.7 1,293,251 + RALBFv3_RS05805 0.81 +1.0 1,293,251 + RALBFv3_RS05805 0.81 +1.5 1,293,469 - -0.0 1,293,479 - +0.8 1,293,479 - -0.3 1,293,479 - +1.4 1,293,506 - +0.4 1,293,556 + +0.5 1,293,556 + +0.5 1,293,556 + -0.3 1,293,556 + +0.3 1,293,556 + +1.6 1,293,557 - -0.1 1,293,586 + +0.7 1,293,587 - +0.2 1,293,587 - +1.4 1,293,587 - +0.4 1,293,587 - +1.0 1,293,787 + RALBFv3_RS05810 0.26 +0.0 1,293,841 + RALBFv3_RS05810 0.32 +0.4 1,293,841 + RALBFv3_RS05810 0.32 +0.0 1,294,003 + RALBFv3_RS05810 0.48 +0.3 1,294,003 + RALBFv3_RS05810 0.48 +0.2 1,294,049 - RALBFv3_RS05810 0.52 +0.2
Or see this region's nucleotide sequence