Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS02745 and RALBFv3_RS02750 are separated by 11 nucleotides RALBFv3_RS02750 and RALBFv3_RS02755 are separated by 36 nucleotides RALBFv3_RS02755 and RALBFv3_RS02760 are separated by 18 nucleotides RALBFv3_RS02760 and RALBFv3_RS02765 are separated by 359 nucleotides
RALBFv3_RS02745: RALBFv3_RS02745 - enoyl-CoA hydratase, at 611,667 to 612,446
_RS02745
RALBFv3_RS02750: RALBFv3_RS02750 - histidine phosphatase family protein, at 612,458 to 613,192
_RS02750
RALBFv3_RS02755: RALBFv3_RS02755 - phosphotransferase family protein, at 613,229 to 614,314
_RS02755
RALBFv3_RS02760: RALBFv3_RS02760 - glyoxalase, at 614,333 to 614,743
_RS02760
RALBFv3_RS02765: RALBFv3_RS02765 - LysR family transcriptional regulator, at 615,103 to 616,077
_RS02765
Position (kb) 613
614
615 Strain fitness (log2 ratio) -1
0
1
2 at 612.297 kb on - strand, within RALBFv3_RS02745 at 612.494 kb on + strand at 612.495 kb on - strand at 612.521 kb on + strand at 612.521 kb on + strand at 612.522 kb on - strand at 613.163 kb on + strand at 613.164 kb on - strand at 613.427 kb on + strand, within RALBFv3_RS02755 at 613.428 kb on - strand, within RALBFv3_RS02755 at 613.428 kb on - strand, within RALBFv3_RS02755 at 613.590 kb on - strand, within RALBFv3_RS02755 at 613.793 kb on + strand, within RALBFv3_RS02755 at 613.793 kb on + strand, within RALBFv3_RS02755 at 613.794 kb on - strand, within RALBFv3_RS02755 at 614.100 kb on - strand, within RALBFv3_RS02755 at 614.100 kb on - strand, within RALBFv3_RS02755 at 614.165 kb on + strand, within RALBFv3_RS02755 at 614.743 kb on + strand at 614.743 kb on + strand at 614.744 kb on - strand at 614.744 kb on - strand at 615.070 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 612,297 - RALBFv3_RS02745 0.81 +0.1 612,494 + +0.7 612,495 - +0.3 612,521 + +2.0 612,521 + -0.7 612,522 - -0.6 613,163 + -1.7 613,164 - -0.8 613,427 + RALBFv3_RS02755 0.18 +0.7 613,428 - RALBFv3_RS02755 0.18 +0.1 613,428 - RALBFv3_RS02755 0.18 +0.6 613,590 - RALBFv3_RS02755 0.33 +0.5 613,793 + RALBFv3_RS02755 0.52 -0.0 613,793 + RALBFv3_RS02755 0.52 +0.2 613,794 - RALBFv3_RS02755 0.52 +0.5 614,100 - RALBFv3_RS02755 0.80 +0.4 614,100 - RALBFv3_RS02755 0.80 -0.2 614,165 + RALBFv3_RS02755 0.86 -0.3 614,743 + +0.1 614,743 + -1.4 614,744 - +1.8 614,744 - -0.6 615,070 - +1.3
Or see this region's nucleotide sequence