Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS17815 and RALBFv3_RS17820 are separated by 102 nucleotides RALBFv3_RS17820 and RALBFv3_RS17825 are separated by 265 nucleotides RALBFv3_RS17825 and RALBFv3_RS17830 are separated by 140 nucleotides
RALBFv3_RS17815: RALBFv3_RS17815 - bifunctional DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase, at 499,508 to 500,344
_RS17815
RALBFv3_RS17820: RALBFv3_RS17820 - RidA family protein, at 500,447 to 500,923
_RS17820
RALBFv3_RS17825: RALBFv3_RS17825 - arylesterase, at 501,189 to 501,830
_RS17825
RALBFv3_RS17830: RALBFv3_RS17830 - 2-dehydropantoate 2-reductase, at 501,971 to 502,999
_RS17830
Position (kb) 501
502 Strain fitness (log2 ratio) -1
0
1 at 500.202 kb on + strand, within RALBFv3_RS17815 at 500.319 kb on + strand at 500.319 kb on + strand at 500.319 kb on + strand at 500.319 kb on + strand at 500.320 kb on - strand at 500.320 kb on - strand at 500.358 kb on + strand at 500.359 kb on - strand at 500.630 kb on + strand, within RALBFv3_RS17820 at 500.630 kb on + strand, within RALBFv3_RS17820 at 500.796 kb on + strand, within RALBFv3_RS17820 at 500.796 kb on + strand, within RALBFv3_RS17820 at 500.797 kb on - strand, within RALBFv3_RS17820 at 500.797 kb on - strand, within RALBFv3_RS17820 at 500.797 kb on - strand, within RALBFv3_RS17820 at 500.817 kb on + strand, within RALBFv3_RS17820 at 500.817 kb on + strand, within RALBFv3_RS17820 at 500.817 kb on + strand, within RALBFv3_RS17820 at 501.067 kb on + strand at 501.858 kb on - strand at 501.863 kb on - strand at 501.863 kb on - strand at 501.929 kb on - strand at 501.989 kb on + strand at 502.013 kb on + strand at 502.013 kb on + strand at 502.013 kb on + strand at 502.014 kb on - strand at 502.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 500,202 + RALBFv3_RS17815 0.83 +0.1 500,319 + -0.2 500,319 + -0.8 500,319 + -0.3 500,319 + +0.8 500,320 - -0.2 500,320 - +1.1 500,358 + +0.0 500,359 - -0.8 500,630 + RALBFv3_RS17820 0.38 -1.0 500,630 + RALBFv3_RS17820 0.38 +1.1 500,796 + RALBFv3_RS17820 0.73 +1.3 500,796 + RALBFv3_RS17820 0.73 +0.3 500,797 - RALBFv3_RS17820 0.73 -0.2 500,797 - RALBFv3_RS17820 0.73 -0.7 500,797 - RALBFv3_RS17820 0.73 -1.7 500,817 + RALBFv3_RS17820 0.78 -1.3 500,817 + RALBFv3_RS17820 0.78 +0.7 500,817 + RALBFv3_RS17820 0.78 +0.9 501,067 + +0.4 501,858 - -0.3 501,863 - +0.5 501,863 - -0.5 501,929 - -0.1 501,989 + +0.2 502,013 + +0.2 502,013 + +0.1 502,013 + -0.1 502,014 - +0.0 502,014 - -0.4
Or see this region's nucleotide sequence