Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS17840 and RALBFv3_RS17845 are separated by 23 nucleotides RALBFv3_RS17845 and RALBFv3_RS24295 are separated by 235 nucleotides RALBFv3_RS24295 and RALBFv3_RS17850 are separated by 792 nucleotides
RALBFv3_RS17840: RALBFv3_RS17840 - cytochrome ubiquinol oxidase subunit I, at 504,021 to 505,409
_RS17840
RALBFv3_RS17845: RALBFv3_RS17845 - GbsR/MarR family transcriptional regulator, at 505,433 to 505,993
_RS17845
RALBFv3_RS24295: RALBFv3_RS24295 - hypothetical protein, at 506,229 to 506,414
_RS24295
RALBFv3_RS17850: RALBFv3_RS17850 - hypothetical protein, at 507,207 to 509,630
_RS17850
Position (kb) 506
507 Strain fitness (log2 ratio) -2
-1
0
1
2 at 505.417 kb on + strand at 505.418 kb on - strand at 505.435 kb on - strand at 505.435 kb on - strand at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.587 kb on + strand, within RALBFv3_RS17845 at 505.749 kb on + strand, within RALBFv3_RS17845 at 505.750 kb on - strand, within RALBFv3_RS17845 at 505.750 kb on - strand, within RALBFv3_RS17845 at 505.890 kb on + strand, within RALBFv3_RS17845 at 505.891 kb on - strand, within RALBFv3_RS17845 at 505.891 kb on - strand, within RALBFv3_RS17845 at 505.902 kb on + strand, within RALBFv3_RS17845 at 505.902 kb on + strand, within RALBFv3_RS17845 at 505.903 kb on - strand, within RALBFv3_RS17845 at 506.166 kb on - strand at 506.166 kb on - strand at 506.166 kb on - strand at 506.166 kb on - strand at 506.637 kb on - strand at 506.721 kb on + strand at 506.880 kb on - strand at 506.950 kb on + strand at 506.976 kb on + strand at 506.976 kb on + strand at 507.220 kb on + strand at 507.220 kb on - strand at 507.221 kb on - strand at 507.221 kb on - strand at 507.221 kb on - strand at 507.236 kb on + strand at 507.237 kb on - strand at 507.380 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 505,417 + -1.9 505,418 - -1.8 505,435 - -0.7 505,435 - -0.2 505,587 + RALBFv3_RS17845 0.27 -2.5 505,587 + RALBFv3_RS17845 0.27 -2.1 505,587 + RALBFv3_RS17845 0.27 -1.3 505,587 + RALBFv3_RS17845 0.27 -1.8 505,587 + RALBFv3_RS17845 0.27 -1.3 505,587 + RALBFv3_RS17845 0.27 -1.0 505,587 + RALBFv3_RS17845 0.27 -1.1 505,587 + RALBFv3_RS17845 0.27 -1.1 505,749 + RALBFv3_RS17845 0.56 +1.0 505,750 - RALBFv3_RS17845 0.57 +0.4 505,750 - RALBFv3_RS17845 0.57 +0.1 505,890 + RALBFv3_RS17845 0.81 -0.2 505,891 - RALBFv3_RS17845 0.82 -0.4 505,891 - RALBFv3_RS17845 0.82 -1.1 505,902 + RALBFv3_RS17845 0.84 -0.6 505,902 + RALBFv3_RS17845 0.84 -2.0 505,903 - RALBFv3_RS17845 0.84 -0.6 506,166 - +0.5 506,166 - -1.1 506,166 - +0.1 506,166 - -0.6 506,637 - +1.0 506,721 + +0.1 506,880 - +0.1 506,950 + +2.0 506,976 + -0.7 506,976 + -0.4 507,220 + -0.6 507,220 - -0.9 507,221 - -0.4 507,221 - +0.7 507,221 - +0.4 507,236 + +0.2 507,237 - +1.0 507,380 + +1.6
Or see this region's nucleotide sequence