Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS15255

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS15250 and RALBFv3_RS15255 are separated by 370 nucleotidesRALBFv3_RS15255 and RALBFv3_RS15260 are separated by 43 nucleotides RALBFv3_RS15250: RALBFv3_RS15250 - BAX inhibitor (BI)-1/YccA family protein, at 3,320,969 to 3,321,670 _RS15250 RALBFv3_RS15255: RALBFv3_RS15255 - nucleoside-diphosphate kinase, at 3,322,041 to 3,322,466 _RS15255 RALBFv3_RS15260: RALBFv3_RS15260 - 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN, at 3,322,510 to 3,323,661 _RS15260 Position (kb) 3322 3323Strain fitness (log2 ratio) -1 0 1at 3321.064 kb on - strand, within RALBFv3_RS15250at 3321.103 kb on - strandat 3321.111 kb on + strand, within RALBFv3_RS15250at 3321.112 kb on - strand, within RALBFv3_RS15250at 3321.865 kb on - strandat 3321.865 kb on - strandat 3322.455 kb on + strandat 3322.455 kb on + strandat 3322.818 kb on + strand, within RALBFv3_RS15260

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
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3,321,064 - RALBFv3_RS15250 0.14 -0.9
3,321,103 - -0.5
3,321,111 + RALBFv3_RS15250 0.20 -1.5
3,321,112 - RALBFv3_RS15250 0.20 -0.3
3,321,865 - +0.2
3,321,865 - -0.2
3,322,455 + +0.5
3,322,455 + -0.2
3,322,818 + RALBFv3_RS15260 0.27 -1.1

Or see this region's nucleotide sequence