Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS01395

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS01390 and RALBFv3_RS01395 are separated by 134 nucleotidesRALBFv3_RS01395 and RALBFv3_RS01400 are separated by 15 nucleotides RALBFv3_RS01390: RALBFv3_RS01390 - efflux RND transporter periplasmic adaptor subunit, at 312,411 to 313,619 _RS01390 RALBFv3_RS01395: RALBFv3_RS01395 - squalene synthase HpnD, at 313,754 to 314,626 _RS01395 RALBFv3_RS01400: RALBFv3_RS01400 - hypothetical protein, at 314,642 to 315,826 _RS01400 Position (kb) 313 314 315Strain fitness (log2 ratio) -2 -1 0 1 2at 312.784 kb on + strand, within RALBFv3_RS01390at 312.785 kb on - strand, within RALBFv3_RS01390at 312.785 kb on - strand, within RALBFv3_RS01390at 313.093 kb on + strand, within RALBFv3_RS01390at 313.115 kb on - strand, within RALBFv3_RS01390at 313.151 kb on + strand, within RALBFv3_RS01390at 313.542 kb on - strandat 313.652 kb on + strandat 313.653 kb on - strandat 313.751 kb on - strandat 313.751 kb on - strandat 313.808 kb on + strandat 313.848 kb on - strand, within RALBFv3_RS01395at 313.850 kb on + strand, within RALBFv3_RS01395at 314.315 kb on + strand, within RALBFv3_RS01395at 314.315 kb on + strand, within RALBFv3_RS01395at 314.316 kb on - strand, within RALBFv3_RS01395at 314.316 kb on - strand, within RALBFv3_RS01395at 314.730 kb on + strandat 314.927 kb on - strand, within RALBFv3_RS01400at 315.025 kb on + strand, within RALBFv3_RS01400at 315.025 kb on + strand, within RALBFv3_RS01400at 315.435 kb on + strand, within RALBFv3_RS01400

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
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312,784 + RALBFv3_RS01390 0.31 -1.2
312,785 - RALBFv3_RS01390 0.31 -0.9
312,785 - RALBFv3_RS01390 0.31 +2.1
313,093 + RALBFv3_RS01390 0.56 -1.2
313,115 - RALBFv3_RS01390 0.58 -0.7
313,151 + RALBFv3_RS01390 0.61 +1.1
313,542 - -2.1
313,652 + -1.9
313,653 - -0.5
313,751 - -0.1
313,751 - -0.5
313,808 + -1.2
313,848 - RALBFv3_RS01395 0.11 +0.5
313,850 + RALBFv3_RS01395 0.11 -0.5
314,315 + RALBFv3_RS01395 0.64 -0.6
314,315 + RALBFv3_RS01395 0.64 +0.5
314,316 - RALBFv3_RS01395 0.64 +1.8
314,316 - RALBFv3_RS01395 0.64 +1.1
314,730 + -0.2
314,927 - RALBFv3_RS01400 0.24 -0.2
315,025 + RALBFv3_RS01400 0.32 -1.0
315,025 + RALBFv3_RS01400 0.32 +0.5
315,435 + RALBFv3_RS01400 0.67 -0.2

Or see this region's nucleotide sequence