Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS01105 and RALBFv3_RS01110 are separated by 32 nucleotides RALBFv3_RS01110 and RALBFv3_RS01115 are separated by 48 nucleotides RALBFv3_RS01115 and RALBFv3_RS01120 are separated by 114 nucleotides
RALBFv3_RS01105: RALBFv3_RS01105 - PaaI family thioesterase, at 243,675 to 244,109
_RS01105
RALBFv3_RS01110: RALBFv3_RS01110 - gluconate 2-dehydrogenase, at 244,142 to 244,924
_RS01110
RALBFv3_RS01115: RALBFv3_RS01115 - acyl-CoA dehydrogenase, at 244,973 to 246,841
_RS01115
RALBFv3_RS01120: RALBFv3_RS01120 - acetyl-CoA acetyltransferase, at 246,956 to 248,134
_RS01120
Position (kb) 244
245
246
247 Strain fitness (log2 ratio) -1
0
1 at 244.365 kb on + strand, within RALBFv3_RS01110 at 244.366 kb on - strand, within RALBFv3_RS01110 at 244.429 kb on - strand, within RALBFv3_RS01110 at 245.496 kb on + strand, within RALBFv3_RS01115 at 245.496 kb on + strand, within RALBFv3_RS01115 at 245.850 kb on + strand, within RALBFv3_RS01115 at 246.267 kb on + strand, within RALBFv3_RS01115 at 246.623 kb on - strand, within RALBFv3_RS01115 at 246.673 kb on + strand at 246.673 kb on + strand at 246.795 kb on + strand at 247.020 kb on + strand at 247.021 kb on - strand at 247.021 kb on - strand at 247.162 kb on - strand, within RALBFv3_RS01120 at 247.659 kb on + strand, within RALBFv3_RS01120
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 244,365 + RALBFv3_RS01110 0.28 -0.0 244,366 - RALBFv3_RS01110 0.29 -1.0 244,429 - RALBFv3_RS01110 0.37 -1.0 245,496 + RALBFv3_RS01115 0.28 +0.5 245,496 + RALBFv3_RS01115 0.28 +0.1 245,850 + RALBFv3_RS01115 0.47 +0.3 246,267 + RALBFv3_RS01115 0.69 -0.1 246,623 - RALBFv3_RS01115 0.88 -0.1 246,673 + -0.2 246,673 + -0.2 246,795 + -0.3 247,020 + +0.5 247,021 - -0.5 247,021 - -0.1 247,162 - RALBFv3_RS01120 0.17 -0.3 247,659 + RALBFv3_RS01120 0.60 +1.5
Or see this region's nucleotide sequence