Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS23060 and RS_RS26365 are separated by 31 nucleotides RS_RS26365 and RS_RS23065 are separated by 141 nucleotides RS_RS23065 and RS_RS23070 are separated by 156 nucleotides
RS_RS23060: RS_RS23060 - LLM class flavin-dependent oxidoreductase, at 1,562,030 to 1,563,004
_RS23060
RS_RS26365: RS_RS26365 - hypothetical protein, at 1,563,036 to 1,563,239
_RS26365
RS_RS23065: RS_RS23065 - hypothetical protein, at 1,563,381 to 1,564,421
_RS23065
RS_RS23070: RS_RS23070 - alpha/beta hydrolase, at 1,564,578 to 1,565,396
_RS23070
Position (kb)
1563
1564
1565 Strain fitness (log2 ratio)
-1
0
1
2 at 1562.860 kb on - strand, within RS_RS23060 at 1563.092 kb on + strand, within RS_RS26365 at 1563.092 kb on + strand, within RS_RS26365 at 1563.092 kb on + strand, within RS_RS26365 at 1563.093 kb on - strand, within RS_RS26365 at 1563.093 kb on - strand, within RS_RS26365 at 1563.093 kb on - strand, within RS_RS26365 at 1563.096 kb on + strand, within RS_RS26365 at 1563.096 kb on + strand, within RS_RS26365 at 1563.097 kb on - strand, within RS_RS26365 at 1563.097 kb on - strand, within RS_RS26365 at 1563.113 kb on + strand, within RS_RS26365 at 1563.114 kb on - strand, within RS_RS26365 at 1563.347 kb on + strand at 1563.348 kb on - strand at 1563.348 kb on - strand at 1563.348 kb on - strand at 1563.478 kb on - strand at 1563.478 kb on - strand at 1563.531 kb on + strand, within RS_RS23065 at 1563.532 kb on - strand, within RS_RS23065 at 1563.532 kb on - strand, within RS_RS23065 at 1563.556 kb on - strand, within RS_RS23065 at 1563.624 kb on + strand, within RS_RS23065 at 1563.625 kb on - strand, within RS_RS23065 at 1563.625 kb on - strand, within RS_RS23065 at 1563.625 kb on - strand, within RS_RS23065 at 1563.625 kb on - strand, within RS_RS23065 at 1564.051 kb on - strand, within RS_RS23065 at 1564.143 kb on + strand, within RS_RS23065 at 1564.147 kb on - strand, within RS_RS23065 at 1564.147 kb on - strand, within RS_RS23065 at 1564.469 kb on - strand at 1564.500 kb on - strand at 1564.500 kb on - strand at 1564.500 kb on - strand at 1564.720 kb on - strand, within RS_RS23070 at 1564.720 kb on - strand, within RS_RS23070 at 1565.089 kb on - strand, within RS_RS23070 at 1565.104 kb on - strand, within RS_RS23070 at 1565.104 kb on - strand, within RS_RS23070 at 1565.235 kb on + strand, within RS_RS23070 at 1565.236 kb on - strand, within RS_RS23070 at 1565.236 kb on - strand, within RS_RS23070 at 1565.331 kb on + strand at 1565.332 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 1,562,860 - RS_RS23060 0.85 +0.9 1,563,092 + RS_RS26365 0.27 +0.9 1,563,092 + RS_RS26365 0.27 +0.3 1,563,092 + RS_RS26365 0.27 -1.0 1,563,093 - RS_RS26365 0.28 +0.2 1,563,093 - RS_RS26365 0.28 -0.4 1,563,093 - RS_RS26365 0.28 +0.2 1,563,096 + RS_RS26365 0.29 -0.4 1,563,096 + RS_RS26365 0.29 +0.6 1,563,097 - RS_RS26365 0.30 +0.0 1,563,097 - RS_RS26365 0.30 -0.4 1,563,113 + RS_RS26365 0.38 +0.3 1,563,114 - RS_RS26365 0.38 +0.1 1,563,347 + -0.2 1,563,348 - +0.3 1,563,348 - +0.2 1,563,348 - +0.1 1,563,478 - +0.3 1,563,478 - -0.2 1,563,531 + RS_RS23065 0.14 +0.2 1,563,532 - RS_RS23065 0.15 -0.2 1,563,532 - RS_RS23065 0.15 +0.0 1,563,556 - RS_RS23065 0.17 +0.2 1,563,624 + RS_RS23065 0.23 -0.1 1,563,625 - RS_RS23065 0.23 -0.8 1,563,625 - RS_RS23065 0.23 +2.2 1,563,625 - RS_RS23065 0.23 +1.0 1,563,625 - RS_RS23065 0.23 -0.3 1,564,051 - RS_RS23065 0.64 -0.7 1,564,143 + RS_RS23065 0.73 +0.5 1,564,147 - RS_RS23065 0.74 -1.4 1,564,147 - RS_RS23065 0.74 -0.2 1,564,469 - +0.6 1,564,500 - -0.0 1,564,500 - +1.2 1,564,500 - -0.3 1,564,720 - RS_RS23070 0.17 -0.0 1,564,720 - RS_RS23070 0.17 -0.6 1,565,089 - RS_RS23070 0.62 -0.3 1,565,104 - RS_RS23070 0.64 +1.2 1,565,104 - RS_RS23070 0.64 +0.3 1,565,235 + RS_RS23070 0.80 -0.3 1,565,236 - RS_RS23070 0.80 +0.7 1,565,236 - RS_RS23070 0.80 +0.0 1,565,331 + +1.0 1,565,332 - -0.3
Or see this region's nucleotide sequence