Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS10215 and RS_RS10220 are separated by 99 nucleotides RS_RS10220 and RS_RS10225 are separated by 13 nucleotides RS_RS10225 and RS_RS10230 overlap by 4 nucleotides
RS_RS10215: RS_RS10215 - urease accessory protein, at 2,209,115 to 2,210,002
_RS10215
RS_RS10220: RS_RS10220 - urea ABC transporter ATP-binding subunit UrtE, at 2,210,102 to 2,210,794
_RS10220
RS_RS10225: RS_RS10225 - urea ABC transporter ATP-binding protein UrtD, at 2,210,808 to 2,211,671
_RS10225
RS_RS10230: RS_RS10230 - urea ABC transporter permease subunit UrtC, at 2,211,668 to 2,212,858
_RS10230
Position (kb) 2210
2211 Strain fitness (log2 ratio) -1
0
1
2
3 at 2209.711 kb on - strand, within RS_RS10215 at 2210.451 kb on + strand, within RS_RS10220 at 2210.451 kb on + strand, within RS_RS10220 at 2210.556 kb on + strand, within RS_RS10220 at 2210.556 kb on + strand, within RS_RS10220 at 2210.556 kb on + strand, within RS_RS10220 at 2210.766 kb on + strand at 2210.767 kb on - strand at 2210.821 kb on + strand at 2211.550 kb on + strand, within RS_RS10225 at 2211.550 kb on + strand, within RS_RS10225
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 2,209,711 - RS_RS10215 0.67 +2.8 2,210,451 + RS_RS10220 0.50 -0.2 2,210,451 + RS_RS10220 0.50 +0.2 2,210,556 + RS_RS10220 0.66 +0.0 2,210,556 + RS_RS10220 0.66 +0.3 2,210,556 + RS_RS10220 0.66 -1.5 2,210,766 + -0.8 2,210,767 - -0.5 2,210,821 + -1.0 2,211,550 + RS_RS10225 0.86 +1.2 2,211,550 + RS_RS10225 0.86 -0.7
Or see this region's nucleotide sequence