Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS18345 and RS_RS18350 are separated by 122 nucleotides RS_RS18350 and RS_RS18355 are separated by 39 nucleotides RS_RS18355 and RS_RS18360 are separated by 103 nucleotides RS_RS18360 and RS_RS18365 overlap by 4 nucleotides
RS_RS18345: RS_RS18345 - ferric iron uptake transcriptional regulator, at 321,184 to 321,639
_RS18345
RS_RS18350: RS_RS18350 - hypothetical protein, at 321,762 to 322,007
_RS18350
RS_RS18355: RS_RS18355 - hypothetical protein, at 322,047 to 322,352
_RS18355
RS_RS18360: RS_RS18360 - hypothetical protein, at 322,456 to 322,773
_RS18360
RS_RS18365: RS_RS18365 - ferrous iron transport protein B, at 322,770 to 324,635
_RS18365
Position (kb) 321
322
323 Strain fitness (log2 ratio) -2
-1
0
1
2 at 320.765 kb on + strand at 320.766 kb on - strand at 320.766 kb on - strand at 320.849 kb on + strand at 320.849 kb on + strand at 320.849 kb on + strand at 320.850 kb on - strand at 320.850 kb on - strand at 320.858 kb on + strand at 320.916 kb on + strand at 321.006 kb on + strand at 321.007 kb on - strand at 321.025 kb on + strand at 321.025 kb on + strand at 321.189 kb on + strand at 321.189 kb on + strand at 321.190 kb on - strand at 321.190 kb on - strand at 321.190 kb on - strand at 321.190 kb on - strand at 321.190 kb on - strand at 321.352 kb on + strand, within RS_RS18345 at 321.400 kb on - strand, within RS_RS18345 at 321.425 kb on - strand, within RS_RS18345 at 321.574 kb on + strand, within RS_RS18345 at 321.574 kb on + strand, within RS_RS18345 at 321.575 kb on - strand, within RS_RS18345 at 321.575 kb on - strand, within RS_RS18345 at 321.575 kb on - strand, within RS_RS18345 at 321.575 kb on - strand, within RS_RS18345 at 321.637 kb on + strand at 321.757 kb on + strand at 321.758 kb on - strand at 321.758 kb on - strand at 321.991 kb on - strand at 321.991 kb on - strand at 322.375 kb on - strand at 322.385 kb on + strand at 322.386 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 320,765 + -0.4 320,766 - -0.3 320,766 - -0.3 320,849 + -1.5 320,849 + +0.1 320,849 + -0.4 320,850 - -0.8 320,850 - +0.1 320,858 + +0.1 320,916 + -0.3 321,006 + +1.7 321,007 - +0.7 321,025 + -0.4 321,025 + -0.6 321,189 + +0.2 321,189 + -0.8 321,190 - -0.3 321,190 - -1.9 321,190 - +1.0 321,190 - -0.9 321,190 - -0.2 321,352 + RS_RS18345 0.37 +1.0 321,400 - RS_RS18345 0.47 +0.2 321,425 - RS_RS18345 0.53 +1.7 321,574 + RS_RS18345 0.86 -0.6 321,574 + RS_RS18345 0.86 -0.2 321,575 - RS_RS18345 0.86 +0.4 321,575 - RS_RS18345 0.86 -0.1 321,575 - RS_RS18345 0.86 +0.2 321,575 - RS_RS18345 0.86 +1.7 321,637 + -1.5 321,757 + +0.3 321,758 - +2.7 321,758 - -0.4 321,991 - -0.3 321,991 - -1.4 322,375 - +0.6 322,385 + +0.2 322,386 - +0.9
Or see this region's nucleotide sequence