Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS17000 and RS_RS17005 are separated by 15 nucleotides RS_RS17005 and RS_RS17010 are separated by 71 nucleotides
RS_RS17000: RS_RS17000 - histidine kinase, at 3,657,889 to 3,659,368
_RS17000
RS_RS17005: RS_RS17005 - IS630 family transposase ISRso5, at 3,659,384 to 3,660,475
_RS17005
RS_RS17010: RS_RS17010 - restriction endonuclease subunit R, at 3,660,547 to 3,663,411
_RS17010
Position (kb)
3659
3660
3661 Strain fitness (log2 ratio)
-1
0
1
2 at 3658.640 kb on + strand, within RS_RS17000 at 3658.640 kb on + strand, within RS_RS17000 at 3658.640 kb on + strand, within RS_RS17000 at 3658.641 kb on - strand, within RS_RS17000 at 3659.055 kb on - strand, within RS_RS17000 at 3659.055 kb on - strand, within RS_RS17000 at 3659.099 kb on + strand, within RS_RS17000 at 3659.099 kb on + strand, within RS_RS17000 at 3659.195 kb on - strand, within RS_RS17000 at 3659.386 kb on - strand at 3659.386 kb on - strand at 3659.386 kb on - strand at 3659.386 kb on - strand at 3659.402 kb on - strand at 3660.510 kb on + strand at 3660.514 kb on + strand at 3660.548 kb on + strand at 3660.548 kb on + strand at 3660.665 kb on + strand at 3660.665 kb on + strand at 3660.665 kb on + strand at 3660.666 kb on - strand at 3660.666 kb on - strand at 3661.327 kb on + strand, within RS_RS17010 at 3661.327 kb on + strand, within RS_RS17010 at 3661.327 kb on + strand, within RS_RS17010 at 3661.327 kb on + strand, within RS_RS17010 at 3661.327 kb on + strand, within RS_RS17010 at 3661.328 kb on - strand, within RS_RS17010 at 3661.328 kb on - strand, within RS_RS17010 at 3661.328 kb on - strand, within RS_RS17010 at 3661.472 kb on + strand, within RS_RS17010 at 3661.472 kb on + strand, within RS_RS17010 at 3661.472 kb on + strand, within RS_RS17010 at 3661.473 kb on - strand, within RS_RS17010
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 3,658,640 + RS_RS17000 0.51 -0.0 3,658,640 + RS_RS17000 0.51 +0.9 3,658,640 + RS_RS17000 0.51 -0.2 3,658,641 - RS_RS17000 0.51 -0.4 3,659,055 - RS_RS17000 0.79 +2.2 3,659,055 - RS_RS17000 0.79 -1.6 3,659,099 + RS_RS17000 0.82 -0.6 3,659,099 + RS_RS17000 0.82 -0.3 3,659,195 - RS_RS17000 0.88 -0.4 3,659,386 - -0.8 3,659,386 - -1.0 3,659,386 - +0.1 3,659,386 - -0.1 3,659,402 - +1.9 3,660,510 + +0.2 3,660,514 + -0.6 3,660,548 + -0.4 3,660,548 + -0.3 3,660,665 + -1.0 3,660,665 + -0.2 3,660,665 + +0.3 3,660,666 - +0.4 3,660,666 - -0.4 3,661,327 + RS_RS17010 0.27 -0.4 3,661,327 + RS_RS17010 0.27 -0.1 3,661,327 + RS_RS17010 0.27 -0.2 3,661,327 + RS_RS17010 0.27 -0.1 3,661,327 + RS_RS17010 0.27 -0.3 3,661,328 - RS_RS17010 0.27 -0.6 3,661,328 - RS_RS17010 0.27 +0.9 3,661,328 - RS_RS17010 0.27 -0.6 3,661,472 + RS_RS17010 0.32 -0.2 3,661,472 + RS_RS17010 0.32 -0.9 3,661,472 + RS_RS17010 0.32 +1.9 3,661,473 - RS_RS17010 0.32 +0.9
Or see this region's nucleotide sequence