Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS15320 and RS_RS15325 are separated by 22 nucleotides RS_RS15325 and RS_RS15330 are separated by 156 nucleotides RS_RS15330 and RS_RS15335 are separated by 161 nucleotides
RS_RS15320: RS_RS15320 - carbohydrate ABC transporter permease, at 3,286,322 to 3,287,152
_RS15320
RS_RS15325: RS_RS15325 - membrane protein, at 3,287,175 to 3,287,456
_RS15325
RS_RS15330: RS_RS15330 - carbohydrate ABC transporter substrate-binding protein, at 3,287,613 to 3,289,355
_RS15330
RS_RS15335: RS_RS15335 - glycerol kinase, at 3,289,517 to 3,291,013
_RS15335
Position (kb)
3287
3288
3289
3290 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3286.715 kb on + strand, within RS_RS15320 at 3286.716 kb on - strand, within RS_RS15320 at 3286.860 kb on - strand, within RS_RS15320 at 3287.117 kb on + strand at 3287.117 kb on + strand at 3287.118 kb on - strand at 3287.118 kb on - strand at 3287.118 kb on - strand at 3287.352 kb on + strand, within RS_RS15325 at 3287.353 kb on - strand, within RS_RS15325 at 3287.598 kb on + strand at 3287.608 kb on + strand at 3287.608 kb on + strand at 3287.609 kb on - strand at 3287.994 kb on + strand, within RS_RS15330 at 3287.995 kb on - strand, within RS_RS15330 at 3287.995 kb on - strand, within RS_RS15330 at 3287.995 kb on - strand, within RS_RS15330 at 3288.036 kb on + strand, within RS_RS15330 at 3288.069 kb on + strand, within RS_RS15330 at 3288.069 kb on + strand, within RS_RS15330 at 3288.069 kb on + strand, within RS_RS15330 at 3288.069 kb on + strand, within RS_RS15330 at 3288.069 kb on + strand, within RS_RS15330 at 3288.069 kb on + strand, within RS_RS15330 at 3288.070 kb on - strand, within RS_RS15330 at 3288.070 kb on - strand, within RS_RS15330 at 3288.070 kb on - strand, within RS_RS15330 at 3288.165 kb on + strand, within RS_RS15330 at 3288.165 kb on + strand, within RS_RS15330 at 3288.166 kb on - strand, within RS_RS15330 at 3288.166 kb on - strand, within RS_RS15330 at 3288.258 kb on + strand, within RS_RS15330 at 3288.297 kb on + strand, within RS_RS15330 at 3288.298 kb on - strand, within RS_RS15330 at 3288.358 kb on - strand, within RS_RS15330 at 3288.372 kb on + strand, within RS_RS15330 at 3288.574 kb on - strand, within RS_RS15330 at 3288.595 kb on - strand, within RS_RS15330 at 3288.682 kb on - strand, within RS_RS15330 at 3288.873 kb on + strand, within RS_RS15330 at 3288.873 kb on + strand, within RS_RS15330 at 3288.963 kb on + strand, within RS_RS15330 at 3288.963 kb on + strand, within RS_RS15330 at 3288.964 kb on - strand, within RS_RS15330 at 3288.987 kb on + strand, within RS_RS15330 at 3289.311 kb on + strand at 3289.432 kb on + strand at 3289.432 kb on + strand at 3289.432 kb on + strand at 3289.433 kb on - strand at 3289.517 kb on - strand at 3289.524 kb on - strand at 3290.058 kb on + strand, within RS_RS15335 at 3290.058 kb on + strand, within RS_RS15335 at 3290.059 kb on - strand, within RS_RS15335 at 3290.301 kb on - strand, within RS_RS15335 at 3290.301 kb on - strand, within RS_RS15335 at 3290.301 kb on - strand, within RS_RS15335 at 3290.301 kb on - strand, within RS_RS15335
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 3,286,715 + RS_RS15320 0.47 +0.9 3,286,716 - RS_RS15320 0.47 -0.7 3,286,860 - RS_RS15320 0.65 +1.9 3,287,117 + +0.0 3,287,117 + +0.1 3,287,118 - +0.3 3,287,118 - -0.3 3,287,118 - -0.7 3,287,352 + RS_RS15325 0.63 +0.6 3,287,353 - RS_RS15325 0.63 -0.1 3,287,598 + -0.6 3,287,608 + -1.0 3,287,608 + +0.3 3,287,609 - -0.6 3,287,994 + RS_RS15330 0.22 -0.6 3,287,995 - RS_RS15330 0.22 -0.4 3,287,995 - RS_RS15330 0.22 +0.1 3,287,995 - RS_RS15330 0.22 -1.0 3,288,036 + RS_RS15330 0.24 +0.1 3,288,069 + RS_RS15330 0.26 +1.9 3,288,069 + RS_RS15330 0.26 -0.1 3,288,069 + RS_RS15330 0.26 -0.5 3,288,069 + RS_RS15330 0.26 -0.9 3,288,069 + RS_RS15330 0.26 -1.4 3,288,069 + RS_RS15330 0.26 +0.3 3,288,070 - RS_RS15330 0.26 -1.2 3,288,070 - RS_RS15330 0.26 -0.7 3,288,070 - RS_RS15330 0.26 -0.4 3,288,165 + RS_RS15330 0.32 -0.7 3,288,165 + RS_RS15330 0.32 -0.1 3,288,166 - RS_RS15330 0.32 -1.2 3,288,166 - RS_RS15330 0.32 -0.5 3,288,258 + RS_RS15330 0.37 -0.6 3,288,297 + RS_RS15330 0.39 -1.3 3,288,298 - RS_RS15330 0.39 +1.9 3,288,358 - RS_RS15330 0.43 +0.0 3,288,372 + RS_RS15330 0.44 -0.5 3,288,574 - RS_RS15330 0.55 -0.4 3,288,595 - RS_RS15330 0.56 +0.1 3,288,682 - RS_RS15330 0.61 -0.1 3,288,873 + RS_RS15330 0.72 -1.2 3,288,873 + RS_RS15330 0.72 +1.9 3,288,963 + RS_RS15330 0.77 +0.9 3,288,963 + RS_RS15330 0.77 -0.6 3,288,964 - RS_RS15330 0.78 -1.0 3,288,987 + RS_RS15330 0.79 +0.5 3,289,311 + -0.7 3,289,432 + -0.4 3,289,432 + -0.4 3,289,432 + +1.9 3,289,433 - -1.0 3,289,517 - +0.9 3,289,524 - -2.3 3,290,058 + RS_RS15335 0.36 +0.6 3,290,058 + RS_RS15335 0.36 -0.3 3,290,059 - RS_RS15335 0.36 -0.0 3,290,301 - RS_RS15335 0.52 -0.3 3,290,301 - RS_RS15335 0.52 -0.4 3,290,301 - RS_RS15335 0.52 +1.9 3,290,301 - RS_RS15335 0.52 -1.0
Or see this region's nucleotide sequence