Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS13765 and RS_RS13770 overlap by 4 nucleotides RS_RS13770 and RS_RS13775 overlap by 4 nucleotides RS_RS13775 and RS_RS13780 overlap by 4 nucleotides
RS_RS13765: RS_RS13765 - D-galactonate dehydratase, at 2,962,960 to 2,964,108
_RS13765
RS_RS13770: RS_RS13770 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 2,964,105 to 2,964,746
_RS13770
RS_RS13775: RS_RS13775 - short-chain dehydrogenase, at 2,964,743 to 2,965,540
_RS13775
RS_RS13780: RS_RS13780 - aldose 1-epimerase, at 2,965,537 to 2,966,427
_RS13780
Position (kb)
2964
2965 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2963.132 kb on + strand, within RS_RS13765 at 2963.133 kb on - strand, within RS_RS13765 at 2963.159 kb on + strand, within RS_RS13765 at 2963.160 kb on - strand, within RS_RS13765 at 2963.160 kb on - strand, within RS_RS13765 at 2963.277 kb on - strand, within RS_RS13765 at 2963.450 kb on + strand, within RS_RS13765 at 2963.450 kb on + strand, within RS_RS13765 at 2963.450 kb on + strand, within RS_RS13765 at 2963.450 kb on + strand, within RS_RS13765 at 2963.451 kb on - strand, within RS_RS13765 at 2963.451 kb on - strand, within RS_RS13765 at 2963.759 kb on + strand, within RS_RS13765 at 2963.759 kb on + strand, within RS_RS13765 at 2963.760 kb on - strand, within RS_RS13765 at 2963.760 kb on - strand, within RS_RS13765 at 2963.802 kb on - strand, within RS_RS13765 at 2964.083 kb on + strand at 2964.083 kb on + strand at 2964.084 kb on - strand at 2964.084 kb on - strand at 2964.142 kb on + strand at 2964.247 kb on + strand, within RS_RS13770 at 2964.753 kb on + strand at 2964.838 kb on - strand, within RS_RS13775 at 2965.078 kb on + strand, within RS_RS13775 at 2965.078 kb on + strand, within RS_RS13775 at 2965.079 kb on - strand, within RS_RS13775 at 2965.079 kb on - strand, within RS_RS13775 at 2965.706 kb on + strand, within RS_RS13780 at 2965.706 kb on + strand, within RS_RS13780 at 2965.706 kb on + strand, within RS_RS13780 at 2965.715 kb on + strand, within RS_RS13780 at 2965.716 kb on - strand, within RS_RS13780
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,963,132 + RS_RS13765 0.15 -0.4 2,963,133 - RS_RS13765 0.15 +2.8 2,963,159 + RS_RS13765 0.17 -0.8 2,963,160 - RS_RS13765 0.17 -0.4 2,963,160 - RS_RS13765 0.17 -0.5 2,963,277 - RS_RS13765 0.28 -0.0 2,963,450 + RS_RS13765 0.43 +0.1 2,963,450 + RS_RS13765 0.43 +0.0 2,963,450 + RS_RS13765 0.43 +0.3 2,963,450 + RS_RS13765 0.43 -0.5 2,963,451 - RS_RS13765 0.43 -0.1 2,963,451 - RS_RS13765 0.43 +0.5 2,963,759 + RS_RS13765 0.70 -0.2 2,963,759 + RS_RS13765 0.70 +0.2 2,963,760 - RS_RS13765 0.70 +0.4 2,963,760 - RS_RS13765 0.70 -0.2 2,963,802 - RS_RS13765 0.73 -1.1 2,964,083 + -0.3 2,964,083 + +0.5 2,964,084 - -0.4 2,964,084 - +1.8 2,964,142 + +0.1 2,964,247 + RS_RS13770 0.22 -2.2 2,964,753 + -0.4 2,964,838 - RS_RS13775 0.12 +0.4 2,965,078 + RS_RS13775 0.42 +0.1 2,965,078 + RS_RS13775 0.42 -0.1 2,965,079 - RS_RS13775 0.42 +1.0 2,965,079 - RS_RS13775 0.42 +0.1 2,965,706 + RS_RS13780 0.19 -0.3 2,965,706 + RS_RS13780 0.19 -1.3 2,965,706 + RS_RS13780 0.19 +0.2 2,965,715 + RS_RS13780 0.20 -0.9 2,965,716 - RS_RS13780 0.20 +0.8
Or see this region's nucleotide sequence