Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS12840

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS12835 and RS_RS12840 are separated by 344 nucleotidesRS_RS12840 and RS_RS12845 are separated by 53 nucleotides RS_RS12835: RS_RS12835 - TetR/AcrR family transcriptional regulator, at 2,768,636 to 2,769,265 _RS12835 RS_RS12840: RS_RS12840 - DUF3313 domain-containing protein, at 2,769,610 to 2,770,272 _RS12840 RS_RS12845: RS_RS12845 - MCE family protein, at 2,770,326 to 2,772,002 _RS12845 Position (kb) 2769 2770 2771Strain fitness (log2 ratio) -3 -2 -1 0 1at 2768.645 kb on - strandat 2768.862 kb on - strand, within RS_RS12835at 2769.526 kb on + strandat 2769.527 kb on - strandat 2769.697 kb on + strand, within RS_RS12840at 2769.697 kb on + strand, within RS_RS12840at 2769.698 kb on - strand, within RS_RS12840at 2769.721 kb on + strand, within RS_RS12840at 2769.721 kb on + strand, within RS_RS12840at 2769.722 kb on - strand, within RS_RS12840at 2769.722 kb on - strand, within RS_RS12840at 2769.722 kb on - strand, within RS_RS12840at 2769.769 kb on + strand, within RS_RS12840at 2769.796 kb on + strand, within RS_RS12840at 2769.797 kb on - strand, within RS_RS12840at 2769.797 kb on - strand, within RS_RS12840at 2769.893 kb on - strand, within RS_RS12840at 2770.052 kb on - strand, within RS_RS12840at 2770.052 kb on - strand, within RS_RS12840at 2770.108 kb on + strand, within RS_RS12840at 2770.108 kb on + strand, within RS_RS12840at 2770.109 kb on - strand, within RS_RS12840at 2770.109 kb on - strand, within RS_RS12840at 2770.123 kb on + strand, within RS_RS12840at 2770.124 kb on - strand, within RS_RS12840at 2770.156 kb on + strand, within RS_RS12840at 2770.157 kb on - strand, within RS_RS12840at 2770.270 kb on + strandat 2770.779 kb on + strand, within RS_RS12845at 2770.780 kb on - strand, within RS_RS12845at 2770.780 kb on - strand, within RS_RS12845at 2770.780 kb on - strand, within RS_RS12845at 2770.780 kb on - strand, within RS_RS12845at 2770.780 kb on - strand, within RS_RS12845at 2770.780 kb on - strand, within RS_RS12845at 2770.932 kb on + strand, within RS_RS12845at 2770.933 kb on - strand, within RS_RS12845at 2770.933 kb on - strand, within RS_RS12845at 2771.031 kb on + strand, within RS_RS12845at 2771.032 kb on - strand, within RS_RS12845

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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2,768,645 - -1.0
2,768,862 - RS_RS12835 0.36 -1.3
2,769,526 + +0.8
2,769,527 - -0.3
2,769,697 + RS_RS12840 0.13 +0.2
2,769,697 + RS_RS12840 0.13 +1.7
2,769,698 - RS_RS12840 0.13 -0.4
2,769,721 + RS_RS12840 0.17 -3.1
2,769,721 + RS_RS12840 0.17 +0.0
2,769,722 - RS_RS12840 0.17 -0.2
2,769,722 - RS_RS12840 0.17 -1.0
2,769,722 - RS_RS12840 0.17 +0.3
2,769,769 + RS_RS12840 0.24 -0.1
2,769,796 + RS_RS12840 0.28 -0.4
2,769,797 - RS_RS12840 0.28 +0.0
2,769,797 - RS_RS12840 0.28 +0.1
2,769,893 - RS_RS12840 0.43 -0.2
2,770,052 - RS_RS12840 0.67 -0.6
2,770,052 - RS_RS12840 0.67 -0.1
2,770,108 + RS_RS12840 0.75 -0.2
2,770,108 + RS_RS12840 0.75 +0.1
2,770,109 - RS_RS12840 0.75 -0.5
2,770,109 - RS_RS12840 0.75 +1.7
2,770,123 + RS_RS12840 0.77 -0.3
2,770,124 - RS_RS12840 0.78 -0.4
2,770,156 + RS_RS12840 0.82 +0.7
2,770,157 - RS_RS12840 0.83 -0.1
2,770,270 + -0.3
2,770,779 + RS_RS12845 0.27 +0.7
2,770,780 - RS_RS12845 0.27 -0.5
2,770,780 - RS_RS12845 0.27 -0.0
2,770,780 - RS_RS12845 0.27 +0.0
2,770,780 - RS_RS12845 0.27 +0.3
2,770,780 - RS_RS12845 0.27 -0.3
2,770,780 - RS_RS12845 0.27 -0.2
2,770,932 + RS_RS12845 0.36 -0.6
2,770,933 - RS_RS12845 0.36 +0.5
2,770,933 - RS_RS12845 0.36 -0.3
2,771,031 + RS_RS12845 0.42 -0.2
2,771,032 - RS_RS12845 0.42 +0.3

Or see this region's nucleotide sequence