Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS08190

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS08180 and RS_RS08185 are separated by 109 nucleotidesRS_RS08185 and RS_RS08190 are separated by 40 nucleotidesRS_RS08190 and RS_RS08195 are separated by 79 nucleotides RS_RS08180: RS_RS08180 - RNA-binding transcriptional accessory protein, at 1,742,266 to 1,744,605 _RS08180 RS_RS08185: RS_RS08185 - hypothetical protein, at 1,744,715 to 1,745,491 _RS08185 RS_RS08190: RS_RS08190 - DUF465 domain-containing protein, at 1,745,532 to 1,745,735 _RS08190 RS_RS08195: RS_RS08195 - ATP-dependent DNA helicase, at 1,745,815 to 1,747,956 _RS08195 Position (kb) 1745 1746Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1744.729 kb on + strandat 1745.300 kb on + strand, within RS_RS08185at 1745.300 kb on + strand, within RS_RS08185at 1745.376 kb on - strand, within RS_RS08185at 1745.376 kb on - strand, within RS_RS08185at 1745.538 kb on - strandat 1745.702 kb on - strand, within RS_RS08190at 1746.073 kb on + strand, within RS_RS08195at 1746.074 kb on - strand, within RS_RS08195at 1746.079 kb on + strand, within RS_RS08195at 1746.080 kb on - strand, within RS_RS08195at 1746.233 kb on - strand, within RS_RS08195at 1746.233 kb on - strand, within RS_RS08195at 1746.439 kb on + strand, within RS_RS08195at 1746.440 kb on - strand, within RS_RS08195at 1746.443 kb on - strand, within RS_RS08195

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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1,744,729 + +0.7
1,745,300 + RS_RS08185 0.75 +0.1
1,745,300 + RS_RS08185 0.75 +1.4
1,745,376 - RS_RS08185 0.85 +1.4
1,745,376 - RS_RS08185 0.85 -0.5
1,745,538 - +1.4
1,745,702 - RS_RS08190 0.83 -0.9
1,746,073 + RS_RS08195 0.12 +0.2
1,746,074 - RS_RS08195 0.12 -0.1
1,746,079 + RS_RS08195 0.12 +0.4
1,746,080 - RS_RS08195 0.12 -1.8
1,746,233 - RS_RS08195 0.20 +0.6
1,746,233 - RS_RS08195 0.20 +3.0
1,746,439 + RS_RS08195 0.29 -0.6
1,746,440 - RS_RS08195 0.29 +2.4
1,746,443 - RS_RS08195 0.29 -0.9

Or see this region's nucleotide sequence