Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01845 and RS_RS01850 are separated by 225 nucleotides RS_RS01850 and RS_RS01855 are separated by 10 nucleotides RS_RS01855 and RS_RS01860 are separated by 10 nucleotides
RS_RS01845: RS_RS01845 - GGDEF domain-containing protein, at 402,412 to 405,330
_RS01845
RS_RS01850: RS_RS01850 - OsmC family peroxiredoxin, at 405,556 to 405,960
_RS01850
RS_RS01855: RS_RS01855 - pirin family protein, at 405,971 to 406,840
_RS01855
RS_RS01860: RS_RS01860 - DNA-binding protein YbiB, at 406,851 to 407,864
_RS01860
Position (kb) 405
406 Strain fitness (log2 ratio) -1
0
1
2 at 404.780 kb on + strand, within RS_RS01845 at 404.780 kb on + strand, within RS_RS01845 at 404.786 kb on + strand, within RS_RS01845 at 404.786 kb on + strand, within RS_RS01845 at 404.809 kb on + strand, within RS_RS01845 at 404.809 kb on + strand, within RS_RS01845 at 404.856 kb on - strand, within RS_RS01845 at 404.856 kb on - strand, within RS_RS01845 at 405.274 kb on + strand at 405.275 kb on - strand at 405.312 kb on - strand at 405.476 kb on + strand at 405.476 kb on + strand at 405.477 kb on - strand at 405.477 kb on - strand at 405.516 kb on - strand at 405.748 kb on + strand, within RS_RS01850 at 405.749 kb on - strand, within RS_RS01850 at 405.811 kb on + strand, within RS_RS01850 at 405.812 kb on - strand, within RS_RS01850 at 405.812 kb on - strand, within RS_RS01850 at 406.147 kb on + strand, within RS_RS01855 at 406.170 kb on - strand, within RS_RS01855 at 406.496 kb on + strand, within RS_RS01855 at 406.496 kb on + strand, within RS_RS01855 at 406.497 kb on - strand, within RS_RS01855 at 406.497 kb on - strand, within RS_RS01855 at 406.497 kb on - strand, within RS_RS01855 at 406.718 kb on - strand, within RS_RS01855 at 406.721 kb on + strand, within RS_RS01855 at 406.721 kb on + strand, within RS_RS01855 at 406.722 kb on - strand, within RS_RS01855 at 406.722 kb on - strand, within RS_RS01855
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 404,780 + RS_RS01845 0.81 -0.4 404,780 + RS_RS01845 0.81 -0.0 404,786 + RS_RS01845 0.81 +0.0 404,786 + RS_RS01845 0.81 +0.2 404,809 + RS_RS01845 0.82 -0.3 404,809 + RS_RS01845 0.82 -0.2 404,856 - RS_RS01845 0.84 +1.5 404,856 - RS_RS01845 0.84 +1.8 405,274 + +1.4 405,275 - +1.8 405,312 - -0.5 405,476 + -0.3 405,476 + -0.8 405,477 - -0.7 405,477 - -0.2 405,516 - +0.2 405,748 + RS_RS01850 0.47 -0.6 405,749 - RS_RS01850 0.48 +1.8 405,811 + RS_RS01850 0.63 -0.6 405,812 - RS_RS01850 0.63 -0.0 405,812 - RS_RS01850 0.63 +1.8 406,147 + RS_RS01855 0.20 +1.8 406,170 - RS_RS01855 0.23 -1.1 406,496 + RS_RS01855 0.60 -0.8 406,496 + RS_RS01855 0.60 +0.1 406,497 - RS_RS01855 0.60 +1.8 406,497 - RS_RS01855 0.60 +0.8 406,497 - RS_RS01855 0.60 -0.1 406,718 - RS_RS01855 0.86 +0.6 406,721 + RS_RS01855 0.86 -0.2 406,721 + RS_RS01855 0.86 -0.2 406,722 - RS_RS01855 0.86 +1.4 406,722 - RS_RS01855 0.86 -0.3
Or see this region's nucleotide sequence