Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01100 and RS_RS01105 are separated by 266 nucleotides RS_RS01105 and RS_RS01110 overlap by 4 nucleotides
RS_RS01100: RS_RS01100 - sigma 54-dependent transcriptional regulator, at 243,896 to 245,503
_RS01100
RS_RS01105: RS_RS01105 - hypothetical protein, at 245,770 to 246,153
_RS01105
RS_RS01110: RS_RS01110 - RtcB family protein, at 246,150 to 247,370
_RS01110
Position (kb) 245
246
247 Strain fitness (log2 ratio) -2
-1
0
1
2 at 245.043 kb on + strand, within RS_RS01100 at 245.044 kb on - strand, within RS_RS01100 at 245.113 kb on - strand, within RS_RS01100 at 245.182 kb on - strand, within RS_RS01100 at 245.208 kb on + strand, within RS_RS01100 at 245.239 kb on - strand, within RS_RS01100 at 245.515 kb on + strand at 245.525 kb on + strand at 245.525 kb on + strand at 245.678 kb on + strand at 245.679 kb on - strand at 245.929 kb on + strand, within RS_RS01105 at 245.929 kb on + strand, within RS_RS01105 at 245.930 kb on - strand, within RS_RS01105 at 245.930 kb on - strand, within RS_RS01105 at 245.959 kb on + strand, within RS_RS01105 at 245.960 kb on - strand, within RS_RS01105 at 245.962 kb on + strand, within RS_RS01105 at 245.963 kb on - strand, within RS_RS01105 at 245.963 kb on - strand, within RS_RS01105 at 246.037 kb on + strand, within RS_RS01105 at 246.038 kb on - strand, within RS_RS01105 at 246.038 kb on - strand, within RS_RS01105 at 246.055 kb on + strand, within RS_RS01105 at 246.055 kb on + strand, within RS_RS01105 at 246.165 kb on + strand at 246.165 kb on + strand at 246.165 kb on + strand at 246.166 kb on - strand at 246.166 kb on - strand at 246.597 kb on + strand, within RS_RS01110 at 246.785 kb on + strand, within RS_RS01110 at 246.817 kb on - strand, within RS_RS01110 at 246.849 kb on + strand, within RS_RS01110 at 246.850 kb on - strand, within RS_RS01110 at 246.879 kb on + strand, within RS_RS01110 at 246.879 kb on + strand, within RS_RS01110 at 246.880 kb on - strand, within RS_RS01110 at 246.880 kb on - strand, within RS_RS01110 at 246.990 kb on + strand, within RS_RS01110 at 246.990 kb on + strand, within RS_RS01110 at 246.990 kb on + strand, within RS_RS01110 at 246.990 kb on + strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110 at 246.991 kb on - strand, within RS_RS01110
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 245,043 + RS_RS01100 0.71 +0.9 245,044 - RS_RS01100 0.71 -1.1 245,113 - RS_RS01100 0.76 +0.8 245,182 - RS_RS01100 0.80 +1.9 245,208 + RS_RS01100 0.82 +1.6 245,239 - RS_RS01100 0.84 -0.3 245,515 + -1.5 245,525 + -0.2 245,525 + +0.2 245,678 + -1.5 245,679 - +0.4 245,929 + RS_RS01105 0.41 +0.5 245,929 + RS_RS01105 0.41 +0.1 245,930 - RS_RS01105 0.42 +1.9 245,930 - RS_RS01105 0.42 -0.1 245,959 + RS_RS01105 0.49 -0.4 245,960 - RS_RS01105 0.49 +0.2 245,962 + RS_RS01105 0.50 +1.9 245,963 - RS_RS01105 0.50 -2.3 245,963 - RS_RS01105 0.50 +0.2 246,037 + RS_RS01105 0.70 -0.4 246,038 - RS_RS01105 0.70 -0.9 246,038 - RS_RS01105 0.70 -0.1 246,055 + RS_RS01105 0.74 -0.3 246,055 + RS_RS01105 0.74 -1.4 246,165 + +0.0 246,165 + +0.5 246,165 + +0.5 246,166 - -0.4 246,166 - -0.4 246,597 + RS_RS01110 0.37 +0.5 246,785 + RS_RS01110 0.52 -0.7 246,817 - RS_RS01110 0.55 +0.9 246,849 + RS_RS01110 0.57 +1.9 246,850 - RS_RS01110 0.57 +0.7 246,879 + RS_RS01110 0.60 +0.2 246,879 + RS_RS01110 0.60 +0.1 246,880 - RS_RS01110 0.60 -1.1 246,880 - RS_RS01110 0.60 -0.3 246,990 + RS_RS01110 0.69 +0.1 246,990 + RS_RS01110 0.69 -0.3 246,990 + RS_RS01110 0.69 -0.2 246,990 + RS_RS01110 0.69 -0.3 246,991 - RS_RS01110 0.69 +1.9 246,991 - RS_RS01110 0.69 -0.1 246,991 - RS_RS01110 0.69 +0.3 246,991 - RS_RS01110 0.69 +0.3 246,991 - RS_RS01110 0.69 -0.1
Or see this region's nucleotide sequence