Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00345 and RS_RS00350 are separated by 253 nucleotides RS_RS00350 and RS_RS00355 are separated by 47 nucleotides
RS_RS00345: RS_RS00345 - LysM peptidoglycan-binding domain-containing protein, at 78,593 to 79,765
_RS00345
RS_RS00350: RS_RS00350 - peptide deformylase, at 80,019 to 80,528
_RS00350
RS_RS00355: RS_RS00355 - glycosyltransferase family 39 protein, at 80,576 to 82,549
_RS00355
Position (kb)
80
81 Strain fitness (log2 ratio)
-1
0
1
2
3 at 79.069 kb on - strand, within RS_RS00345 at 79.120 kb on - strand, within RS_RS00345 at 79.198 kb on + strand, within RS_RS00345 at 79.199 kb on - strand, within RS_RS00345 at 79.200 kb on + strand, within RS_RS00345 at 79.200 kb on + strand, within RS_RS00345 at 79.200 kb on + strand, within RS_RS00345 at 79.200 kb on + strand, within RS_RS00345 at 79.201 kb on - strand, within RS_RS00345 at 79.201 kb on - strand, within RS_RS00345 at 79.201 kb on - strand, within RS_RS00345 at 79.201 kb on - strand, within RS_RS00345 at 79.201 kb on - strand, within RS_RS00345 at 79.201 kb on - strand, within RS_RS00345 at 79.446 kb on + strand, within RS_RS00345 at 79.447 kb on - strand, within RS_RS00345 at 79.447 kb on - strand, within RS_RS00345 at 79.522 kb on - strand, within RS_RS00345 at 79.566 kb on + strand, within RS_RS00345 at 79.567 kb on - strand, within RS_RS00345 at 79.567 kb on - strand, within RS_RS00345 at 79.775 kb on - strand at 79.787 kb on + strand at 79.788 kb on - strand at 79.788 kb on - strand at 79.818 kb on + strand at 79.822 kb on + strand at 79.822 kb on + strand at 79.823 kb on - strand at 80.457 kb on + strand, within RS_RS00350 at 80.458 kb on - strand, within RS_RS00350 at 80.635 kb on - strand at 80.726 kb on + strand at 80.749 kb on + strand at 80.822 kb on + strand, within RS_RS00355 at 80.822 kb on + strand, within RS_RS00355 at 81.068 kb on + strand, within RS_RS00355 at 81.068 kb on + strand, within RS_RS00355 at 81.068 kb on + strand, within RS_RS00355 at 81.380 kb on + strand, within RS_RS00355 at 81.484 kb on + strand, within RS_RS00355
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 79,069 - RS_RS00345 0.41 +0.9 79,120 - RS_RS00345 0.45 -0.1 79,198 + RS_RS00345 0.52 +1.7 79,199 - RS_RS00345 0.52 -0.0 79,200 + RS_RS00345 0.52 -0.3 79,200 + RS_RS00345 0.52 +0.7 79,200 + RS_RS00345 0.52 -0.5 79,200 + RS_RS00345 0.52 -0.6 79,201 - RS_RS00345 0.52 +0.9 79,201 - RS_RS00345 0.52 +0.1 79,201 - RS_RS00345 0.52 -1.7 79,201 - RS_RS00345 0.52 -0.1 79,201 - RS_RS00345 0.52 -0.7 79,201 - RS_RS00345 0.52 -0.3 79,446 + RS_RS00345 0.73 -0.8 79,447 - RS_RS00345 0.73 -0.1 79,447 - RS_RS00345 0.73 -0.7 79,522 - RS_RS00345 0.79 +0.7 79,566 + RS_RS00345 0.83 +1.3 79,567 - RS_RS00345 0.83 +1.9 79,567 - RS_RS00345 0.83 +0.9 79,775 - +0.2 79,787 + -0.1 79,788 - -0.1 79,788 - -0.9 79,818 + -0.6 79,822 + +0.9 79,822 + -0.9 79,823 - +2.9 80,457 + RS_RS00350 0.86 +0.9 80,458 - RS_RS00350 0.86 -0.4 80,635 - +0.9 80,726 + -0.7 80,749 + -0.8 80,822 + RS_RS00355 0.12 -0.7 80,822 + RS_RS00355 0.12 -0.4 81,068 + RS_RS00355 0.25 -0.9 81,068 + RS_RS00355 0.25 -1.1 81,068 + RS_RS00355 0.25 -0.8 81,380 + RS_RS00355 0.41 -0.9 81,484 + RS_RS00355 0.46 -0.8
Or see this region's nucleotide sequence