Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS00350

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS00345 and RS_RS00350 are separated by 253 nucleotidesRS_RS00350 and RS_RS00355 are separated by 47 nucleotides RS_RS00345: RS_RS00345 - LysM peptidoglycan-binding domain-containing protein, at 78,593 to 79,765 _RS00345 RS_RS00350: RS_RS00350 - peptide deformylase, at 80,019 to 80,528 _RS00350 RS_RS00355: RS_RS00355 - glycosyltransferase family 39 protein, at 80,576 to 82,549 _RS00355 Position (kb) 80 81Strain fitness (log2 ratio) -1 0 1 2 3at 79.069 kb on - strand, within RS_RS00345at 79.120 kb on - strand, within RS_RS00345at 79.198 kb on + strand, within RS_RS00345at 79.199 kb on - strand, within RS_RS00345at 79.200 kb on + strand, within RS_RS00345at 79.200 kb on + strand, within RS_RS00345at 79.200 kb on + strand, within RS_RS00345at 79.200 kb on + strand, within RS_RS00345at 79.201 kb on - strand, within RS_RS00345at 79.201 kb on - strand, within RS_RS00345at 79.201 kb on - strand, within RS_RS00345at 79.201 kb on - strand, within RS_RS00345at 79.201 kb on - strand, within RS_RS00345at 79.201 kb on - strand, within RS_RS00345at 79.446 kb on + strand, within RS_RS00345at 79.447 kb on - strand, within RS_RS00345at 79.447 kb on - strand, within RS_RS00345at 79.522 kb on - strand, within RS_RS00345at 79.566 kb on + strand, within RS_RS00345at 79.567 kb on - strand, within RS_RS00345at 79.567 kb on - strand, within RS_RS00345at 79.775 kb on - strandat 79.787 kb on + strandat 79.788 kb on - strandat 79.788 kb on - strandat 79.818 kb on + strandat 79.822 kb on + strandat 79.822 kb on + strandat 79.823 kb on - strandat 80.457 kb on + strand, within RS_RS00350at 80.458 kb on - strand, within RS_RS00350at 80.635 kb on - strandat 80.726 kb on + strandat 80.749 kb on + strandat 80.822 kb on + strand, within RS_RS00355at 80.822 kb on + strand, within RS_RS00355at 81.068 kb on + strand, within RS_RS00355at 81.068 kb on + strand, within RS_RS00355at 81.068 kb on + strand, within RS_RS00355at 81.380 kb on + strand, within RS_RS00355at 81.484 kb on + strand, within RS_RS00355

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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79,069 - RS_RS00345 0.41 +0.9
79,120 - RS_RS00345 0.45 -0.1
79,198 + RS_RS00345 0.52 +1.7
79,199 - RS_RS00345 0.52 -0.0
79,200 + RS_RS00345 0.52 -0.3
79,200 + RS_RS00345 0.52 +0.7
79,200 + RS_RS00345 0.52 -0.5
79,200 + RS_RS00345 0.52 -0.6
79,201 - RS_RS00345 0.52 +0.9
79,201 - RS_RS00345 0.52 +0.1
79,201 - RS_RS00345 0.52 -1.7
79,201 - RS_RS00345 0.52 -0.1
79,201 - RS_RS00345 0.52 -0.7
79,201 - RS_RS00345 0.52 -0.3
79,446 + RS_RS00345 0.73 -0.8
79,447 - RS_RS00345 0.73 -0.1
79,447 - RS_RS00345 0.73 -0.7
79,522 - RS_RS00345 0.79 +0.7
79,566 + RS_RS00345 0.83 +1.3
79,567 - RS_RS00345 0.83 +1.9
79,567 - RS_RS00345 0.83 +0.9
79,775 - +0.2
79,787 + -0.1
79,788 - -0.1
79,788 - -0.9
79,818 + -0.6
79,822 + +0.9
79,822 + -0.9
79,823 - +2.9
80,457 + RS_RS00350 0.86 +0.9
80,458 - RS_RS00350 0.86 -0.4
80,635 - +0.9
80,726 + -0.7
80,749 + -0.8
80,822 + RS_RS00355 0.12 -0.7
80,822 + RS_RS00355 0.12 -0.4
81,068 + RS_RS00355 0.25 -0.9
81,068 + RS_RS00355 0.25 -1.1
81,068 + RS_RS00355 0.25 -0.8
81,380 + RS_RS00355 0.41 -0.9
81,484 + RS_RS00355 0.46 -0.8

Or see this region's nucleotide sequence