Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00210 and RS_RS00215 are separated by 34 nucleotides RS_RS00215 and RS_RS00220 are separated by 145 nucleotides RS_RS00220 and RS_RS00225 are separated by 100 nucleotides
RS_RS00210: RS_RS00210 - HslU--HslV peptidase ATPase subunit, at 45,560 to 46,891
_RS00210
RS_RS00215: RS_RS00215 - HslU--HslV peptidase proteolytic subunit, at 46,926 to 47,462
_RS00215
RS_RS00220: RS_RS00220 - ABC transporter substrate-binding protein, at 47,608 to 49,200
_RS00220
RS_RS00225: RS_RS00225 - GTP-binding protein, at 49,301 to 50,599
_RS00225
Position (kb) 47
48
49
50 Strain fitness (log2 ratio) -2
-1
0
1
2 at 46.963 kb on + strand at 46.963 kb on + strand at 46.963 kb on + strand at 46.963 kb on + strand at 46.964 kb on - strand at 46.964 kb on - strand at 47.063 kb on - strand, within RS_RS00215 at 47.245 kb on + strand, within RS_RS00215 at 47.332 kb on + strand, within RS_RS00215 at 47.332 kb on + strand, within RS_RS00215 at 47.333 kb on - strand, within RS_RS00215 at 47.333 kb on - strand, within RS_RS00215 at 47.333 kb on - strand, within RS_RS00215 at 47.452 kb on + strand at 47.452 kb on + strand at 47.452 kb on + strand at 47.453 kb on - strand at 47.523 kb on + strand at 47.524 kb on - strand at 47.524 kb on - strand at 47.524 kb on - strand at 47.524 kb on - strand at 47.699 kb on + strand at 47.699 kb on + strand at 47.699 kb on + strand at 47.699 kb on + strand at 47.700 kb on - strand at 47.700 kb on - strand at 47.700 kb on - strand at 47.700 kb on - strand at 47.867 kb on + strand, within RS_RS00220 at 47.867 kb on + strand, within RS_RS00220 at 47.868 kb on - strand, within RS_RS00220 at 47.868 kb on - strand, within RS_RS00220 at 48.377 kb on + strand, within RS_RS00220 at 48.378 kb on - strand, within RS_RS00220 at 48.378 kb on - strand, within RS_RS00220 at 48.378 kb on - strand, within RS_RS00220 at 48.378 kb on - strand, within RS_RS00220 at 48.563 kb on + strand, within RS_RS00220 at 48.620 kb on + strand, within RS_RS00220 at 48.621 kb on - strand, within RS_RS00220 at 48.701 kb on + strand, within RS_RS00220 at 48.701 kb on + strand, within RS_RS00220 at 48.701 kb on + strand, within RS_RS00220 at 48.702 kb on - strand, within RS_RS00220 at 48.702 kb on - strand, within RS_RS00220 at 48.702 kb on - strand, within RS_RS00220 at 48.803 kb on + strand, within RS_RS00220 at 48.804 kb on - strand, within RS_RS00220 at 48.804 kb on - strand, within RS_RS00220 at 48.804 kb on - strand, within RS_RS00220 at 48.804 kb on - strand, within RS_RS00220 at 48.804 kb on - strand, within RS_RS00220 at 48.812 kb on + strand, within RS_RS00220 at 48.812 kb on + strand, within RS_RS00220 at 48.813 kb on - strand, within RS_RS00220 at 48.813 kb on - strand, within RS_RS00220 at 48.857 kb on + strand, within RS_RS00220 at 48.857 kb on + strand, within RS_RS00220 at 48.857 kb on + strand, within RS_RS00220 at 48.858 kb on - strand, within RS_RS00220 at 49.219 kb on + strand at 49.710 kb on + strand, within RS_RS00225 at 49.711 kb on - strand, within RS_RS00225 at 49.756 kb on + strand, within RS_RS00225 at 49.756 kb on + strand, within RS_RS00225 at 50.154 kb on + strand, within RS_RS00225 at 50.155 kb on - strand, within RS_RS00225
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 46,963 + -0.1 46,963 + -0.1 46,963 + -0.3 46,963 + +0.2 46,964 - +0.5 46,964 - -0.2 47,063 - RS_RS00215 0.26 -0.1 47,245 + RS_RS00215 0.59 -0.4 47,332 + RS_RS00215 0.76 -1.1 47,332 + RS_RS00215 0.76 -0.5 47,333 - RS_RS00215 0.76 +0.9 47,333 - RS_RS00215 0.76 +0.3 47,333 - RS_RS00215 0.76 -1.0 47,452 + +0.2 47,452 + -0.3 47,452 + -0.2 47,453 - -0.5 47,523 + +0.0 47,524 - +0.9 47,524 - -0.2 47,524 - -0.9 47,524 - -0.9 47,699 + -0.1 47,699 + +0.1 47,699 + -0.4 47,699 + -0.3 47,700 - -2.4 47,700 - -0.1 47,700 - -0.2 47,700 - +1.9 47,867 + RS_RS00220 0.16 +0.3 47,867 + RS_RS00220 0.16 +0.3 47,868 - RS_RS00220 0.16 +0.4 47,868 - RS_RS00220 0.16 +0.9 48,377 + RS_RS00220 0.48 +0.1 48,378 - RS_RS00220 0.48 -0.3 48,378 - RS_RS00220 0.48 +0.0 48,378 - RS_RS00220 0.48 -0.6 48,378 - RS_RS00220 0.48 -0.5 48,563 + RS_RS00220 0.60 -0.0 48,620 + RS_RS00220 0.64 -0.4 48,621 - RS_RS00220 0.64 -0.4 48,701 + RS_RS00220 0.69 -0.1 48,701 + RS_RS00220 0.69 -0.3 48,701 + RS_RS00220 0.69 -0.7 48,702 - RS_RS00220 0.69 +0.2 48,702 - RS_RS00220 0.69 -0.3 48,702 - RS_RS00220 0.69 -0.4 48,803 + RS_RS00220 0.75 +0.9 48,804 - RS_RS00220 0.75 -0.5 48,804 - RS_RS00220 0.75 -1.3 48,804 - RS_RS00220 0.75 +0.5 48,804 - RS_RS00220 0.75 +1.9 48,804 - RS_RS00220 0.75 -0.7 48,812 + RS_RS00220 0.76 -0.9 48,812 + RS_RS00220 0.76 +1.0 48,813 - RS_RS00220 0.76 +0.3 48,813 - RS_RS00220 0.76 -0.4 48,857 + RS_RS00220 0.78 -0.9 48,857 + RS_RS00220 0.78 -1.0 48,857 + RS_RS00220 0.78 -0.5 48,858 - RS_RS00220 0.78 -0.2 49,219 + -0.5 49,710 + RS_RS00225 0.31 -0.7 49,711 - RS_RS00225 0.32 -1.8 49,756 + RS_RS00225 0.35 -0.2 49,756 + RS_RS00225 0.35 -1.1 50,154 + RS_RS00225 0.66 -0.1 50,155 - RS_RS00225 0.66 -0.8
Or see this region's nucleotide sequence