Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS00210

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS00205 and RS_RS00210 are separated by 217 nucleotidesRS_RS00210 and RS_RS00215 are separated by 34 nucleotidesRS_RS00215 and RS_RS00220 are separated by 145 nucleotides RS_RS00205: RS_RS00205 - hypothetical protein, at 44,050 to 45,342 _RS00205 RS_RS00210: RS_RS00210 - HslU--HslV peptidase ATPase subunit, at 45,560 to 46,891 _RS00210 RS_RS00215: RS_RS00215 - HslU--HslV peptidase proteolytic subunit, at 46,926 to 47,462 _RS00215 RS_RS00220: RS_RS00220 - ABC transporter substrate-binding protein, at 47,608 to 49,200 _RS00220 Position (kb) 45 46 47Strain fitness (log2 ratio) -2 -1 0 1 2at 44.608 kb on + strand, within RS_RS00205at 44.609 kb on - strand, within RS_RS00205at 44.761 kb on + strand, within RS_RS00205at 44.762 kb on - strand, within RS_RS00205at 44.762 kb on - strand, within RS_RS00205at 44.875 kb on + strand, within RS_RS00205at 44.876 kb on - strand, within RS_RS00205at 44.876 kb on - strand, within RS_RS00205at 44.876 kb on - strand, within RS_RS00205at 44.876 kb on - strand, within RS_RS00205at 44.876 kb on - strand, within RS_RS00205at 44.959 kb on - strand, within RS_RS00205at 45.025 kb on + strand, within RS_RS00205at 45.026 kb on - strand, within RS_RS00205at 45.026 kb on - strand, within RS_RS00205at 45.100 kb on + strand, within RS_RS00205at 45.100 kb on + strand, within RS_RS00205at 45.561 kb on + strandat 45.561 kb on + strandat 45.570 kb on + strandat 45.570 kb on + strandat 45.571 kb on - strandat 45.621 kb on + strandat 45.705 kb on + strand, within RS_RS00210at 45.706 kb on - strand, within RS_RS00210at 45.706 kb on - strand, within RS_RS00210at 45.706 kb on - strand, within RS_RS00210at 45.828 kb on + strand, within RS_RS00210at 45.828 kb on + strand, within RS_RS00210at 45.829 kb on - strand, within RS_RS00210at 45.829 kb on - strand, within RS_RS00210at 46.009 kb on + strand, within RS_RS00210at 46.011 kb on + strand, within RS_RS00210at 46.012 kb on - strand, within RS_RS00210at 46.963 kb on + strandat 46.963 kb on + strandat 46.963 kb on + strandat 46.963 kb on + strandat 46.964 kb on - strandat 46.964 kb on - strandat 47.063 kb on - strand, within RS_RS00215at 47.245 kb on + strand, within RS_RS00215at 47.332 kb on + strand, within RS_RS00215at 47.332 kb on + strand, within RS_RS00215at 47.333 kb on - strand, within RS_RS00215at 47.333 kb on - strand, within RS_RS00215at 47.333 kb on - strand, within RS_RS00215at 47.452 kb on + strandat 47.452 kb on + strandat 47.452 kb on + strandat 47.453 kb on - strandat 47.523 kb on + strandat 47.524 kb on - strandat 47.524 kb on - strandat 47.524 kb on - strandat 47.524 kb on - strandat 47.699 kb on + strandat 47.699 kb on + strandat 47.699 kb on + strandat 47.699 kb on + strandat 47.700 kb on - strandat 47.700 kb on - strandat 47.700 kb on - strandat 47.700 kb on - strandat 47.867 kb on + strand, within RS_RS00220at 47.867 kb on + strand, within RS_RS00220at 47.868 kb on - strand, within RS_RS00220at 47.868 kb on - strand, within RS_RS00220

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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44,608 + RS_RS00205 0.43 +0.2
44,609 - RS_RS00205 0.43 +0.2
44,761 + RS_RS00205 0.55 -0.4
44,762 - RS_RS00205 0.55 +0.6
44,762 - RS_RS00205 0.55 -0.6
44,875 + RS_RS00205 0.64 +0.9
44,876 - RS_RS00205 0.64 -0.4
44,876 - RS_RS00205 0.64 -0.4
44,876 - RS_RS00205 0.64 -0.7
44,876 - RS_RS00205 0.64 +0.9
44,876 - RS_RS00205 0.64 -1.3
44,959 - RS_RS00205 0.70 -1.2
45,025 + RS_RS00205 0.75 -1.2
45,026 - RS_RS00205 0.75 +2.5
45,026 - RS_RS00205 0.75 -1.8
45,100 + RS_RS00205 0.81 +0.9
45,100 + RS_RS00205 0.81 -0.2
45,561 + +0.0
45,561 + -0.3
45,570 + -1.7
45,570 + -1.6
45,571 - -0.2
45,621 + -0.8
45,705 + RS_RS00210 0.11 -0.2
45,706 - RS_RS00210 0.11 -0.2
45,706 - RS_RS00210 0.11 +0.2
45,706 - RS_RS00210 0.11 +0.2
45,828 + RS_RS00210 0.20 +0.6
45,828 + RS_RS00210 0.20 +0.1
45,829 - RS_RS00210 0.20 +0.5
45,829 - RS_RS00210 0.20 -0.0
46,009 + RS_RS00210 0.34 -0.7
46,011 + RS_RS00210 0.34 +0.9
46,012 - RS_RS00210 0.34 -0.5
46,963 + -0.1
46,963 + -0.1
46,963 + -0.3
46,963 + +0.2
46,964 - +0.5
46,964 - -0.2
47,063 - RS_RS00215 0.26 -0.1
47,245 + RS_RS00215 0.59 -0.4
47,332 + RS_RS00215 0.76 -1.1
47,332 + RS_RS00215 0.76 -0.5
47,333 - RS_RS00215 0.76 +0.9
47,333 - RS_RS00215 0.76 +0.3
47,333 - RS_RS00215 0.76 -1.0
47,452 + +0.2
47,452 + -0.3
47,452 + -0.2
47,453 - -0.5
47,523 + +0.0
47,524 - +0.9
47,524 - -0.2
47,524 - -0.9
47,524 - -0.9
47,699 + -0.1
47,699 + +0.1
47,699 + -0.4
47,699 + -0.3
47,700 - -2.4
47,700 - -0.1
47,700 - -0.2
47,700 - +1.9
47,867 + RS_RS00220 0.16 +0.3
47,867 + RS_RS00220 0.16 +0.3
47,868 - RS_RS00220 0.16 +0.4
47,868 - RS_RS00220 0.16 +0.9

Or see this region's nucleotide sequence