Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS21045 and RS_RS26260 are separated by 508 nucleotides RS_RS26260 and RS_RS21055 are separated by 31 nucleotides
RS_RS21045: RS_RS21045 - GntR family transcriptional regulator, at 1,037,705 to 1,038,583
_RS21045
RS_RS26260: RS_RS26260 - hypothetical protein, at 1,039,092 to 1,039,958
_RS26260
RS_RS21055: RS_RS21055 - LysR family transcriptional regulator, at 1,039,990 to 1,040,886
_RS21055
Position (kb)
1039
1040 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1038.229 kb on - strand, within RS_RS21045 at 1038.229 kb on - strand, within RS_RS21045 at 1038.525 kb on + strand at 1038.526 kb on - strand at 1038.526 kb on - strand at 1038.526 kb on - strand at 1038.526 kb on - strand at 1038.546 kb on + strand at 1038.546 kb on + strand at 1038.546 kb on + strand at 1038.546 kb on + strand at 1038.546 kb on + strand at 1038.547 kb on - strand at 1038.547 kb on - strand at 1038.614 kb on + strand at 1038.614 kb on + strand at 1038.718 kb on + strand at 1038.719 kb on - strand at 1038.900 kb on + strand at 1038.900 kb on + strand at 1038.900 kb on + strand at 1038.900 kb on + strand at 1038.901 kb on - strand at 1039.048 kb on + strand at 1039.049 kb on - strand at 1039.049 kb on - strand at 1039.238 kb on - strand, within RS_RS26260 at 1039.374 kb on + strand, within RS_RS26260 at 1039.374 kb on + strand, within RS_RS26260 at 1039.374 kb on + strand, within RS_RS26260 at 1039.375 kb on - strand, within RS_RS26260 at 1039.375 kb on - strand, within RS_RS26260 at 1039.375 kb on - strand, within RS_RS26260 at 1039.375 kb on - strand, within RS_RS26260 at 1039.375 kb on - strand, within RS_RS26260 at 1039.376 kb on + strand, within RS_RS26260 at 1039.377 kb on - strand, within RS_RS26260 at 1039.473 kb on + strand, within RS_RS26260 at 1039.474 kb on - strand, within RS_RS26260 at 1039.495 kb on - strand, within RS_RS26260 at 1039.551 kb on + strand, within RS_RS26260 at 1039.551 kb on + strand, within RS_RS26260 at 1039.551 kb on + strand, within RS_RS26260 at 1039.551 kb on + strand, within RS_RS26260 at 1039.551 kb on + strand, within RS_RS26260 at 1039.552 kb on - strand, within RS_RS26260 at 1039.665 kb on + strand, within RS_RS26260 at 1039.666 kb on - strand, within RS_RS26260 at 1039.666 kb on - strand, within RS_RS26260 at 1039.755 kb on + strand, within RS_RS26260 at 1039.756 kb on - strand, within RS_RS26260 at 1040.117 kb on + strand, within RS_RS21055 at 1040.117 kb on + strand, within RS_RS21055 at 1040.117 kb on + strand, within RS_RS21055 at 1040.118 kb on - strand, within RS_RS21055 at 1040.118 kb on - strand, within RS_RS21055 at 1040.219 kb on + strand, within RS_RS21055 at 1040.220 kb on - strand, within RS_RS21055 at 1040.294 kb on + strand, within RS_RS21055 at 1040.303 kb on + strand, within RS_RS21055 at 1040.304 kb on - strand, within RS_RS21055 at 1040.304 kb on - strand, within RS_RS21055 at 1040.304 kb on - strand, within RS_RS21055 at 1040.304 kb on - strand, within RS_RS21055 at 1040.304 kb on - strand, within RS_RS21055 at 1040.304 kb on - strand, within RS_RS21055 at 1040.899 kb on + strand at 1040.900 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 1,038,229 - RS_RS21045 0.60 -0.3 1,038,229 - RS_RS21045 0.60 -0.3 1,038,525 + +0.2 1,038,526 - -0.3 1,038,526 - +0.9 1,038,526 - -0.1 1,038,526 - +1.7 1,038,546 + +0.7 1,038,546 + -0.3 1,038,546 + +0.3 1,038,546 + -0.6 1,038,546 + -1.3 1,038,547 - +1.2 1,038,547 - +2.1 1,038,614 + -0.1 1,038,614 + +0.5 1,038,718 + +0.3 1,038,719 - -0.1 1,038,900 + +0.6 1,038,900 + -0.2 1,038,900 + -1.3 1,038,900 + -0.3 1,038,901 - +0.0 1,039,048 + -0.1 1,039,049 - -0.9 1,039,049 - -0.3 1,039,238 - RS_RS26260 0.17 -0.5 1,039,374 + RS_RS26260 0.33 -0.2 1,039,374 + RS_RS26260 0.33 -0.2 1,039,374 + RS_RS26260 0.33 -0.9 1,039,375 - RS_RS26260 0.33 -0.3 1,039,375 - RS_RS26260 0.33 +0.9 1,039,375 - RS_RS26260 0.33 -0.1 1,039,375 - RS_RS26260 0.33 -0.9 1,039,375 - RS_RS26260 0.33 +0.4 1,039,376 + RS_RS26260 0.33 -1.3 1,039,377 - RS_RS26260 0.33 +0.3 1,039,473 + RS_RS26260 0.44 +0.7 1,039,474 - RS_RS26260 0.44 +0.7 1,039,495 - RS_RS26260 0.46 +0.7 1,039,551 + RS_RS26260 0.53 +2.0 1,039,551 + RS_RS26260 0.53 +2.1 1,039,551 + RS_RS26260 0.53 -1.3 1,039,551 + RS_RS26260 0.53 -0.6 1,039,551 + RS_RS26260 0.53 +1.0 1,039,552 - RS_RS26260 0.53 -0.3 1,039,665 + RS_RS26260 0.66 +0.3 1,039,666 - RS_RS26260 0.66 -0.4 1,039,666 - RS_RS26260 0.66 +0.1 1,039,755 + RS_RS26260 0.76 -0.3 1,039,756 - RS_RS26260 0.77 -0.2 1,040,117 + RS_RS21055 0.14 -0.0 1,040,117 + RS_RS21055 0.14 -0.6 1,040,117 + RS_RS21055 0.14 +0.3 1,040,118 - RS_RS21055 0.14 +0.1 1,040,118 - RS_RS21055 0.14 +0.3 1,040,219 + RS_RS21055 0.26 -0.1 1,040,220 - RS_RS21055 0.26 -0.8 1,040,294 + RS_RS21055 0.34 +0.1 1,040,303 + RS_RS21055 0.35 +0.2 1,040,304 - RS_RS21055 0.35 +1.1 1,040,304 - RS_RS21055 0.35 -0.2 1,040,304 - RS_RS21055 0.35 +0.2 1,040,304 - RS_RS21055 0.35 -0.6 1,040,304 - RS_RS21055 0.35 -2.1 1,040,304 - RS_RS21055 0.35 -0.1 1,040,899 + -0.4 1,040,900 - -0.5
Or see this region's nucleotide sequence