Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS19500 and RS_RS19505 are separated by 118 nucleotides RS_RS19505 and RS_RS19510 overlap by 4 nucleotides RS_RS19510 and RS_RS19515 are separated by 269 nucleotides
RS_RS19500: RS_RS19500 - hypothetical protein, at 608,100 to 609,632
_RS19500
RS_RS19505: RS_RS19505 - two-component sensor histidine kinase, at 609,751 to 611,148
_RS19505
RS_RS19510: RS_RS19510 - DNA-binding response regulator, at 611,145 to 611,819
_RS19510
RS_RS19515: RS_RS19515 - hypothetical protein, at 612,089 to 612,394
_RS19515
Position (kb) 609
610
611
612 Strain fitness (log2 ratio) -2
-1
0
1 at 609.375 kb on + strand, within RS_RS19500 at 609.375 kb on + strand, within RS_RS19500 at 609.487 kb on + strand at 609.487 kb on + strand at 609.487 kb on + strand at 609.488 kb on - strand at 609.488 kb on - strand at 609.488 kb on - strand at 609.488 kb on - strand at 609.591 kb on + strand at 609.592 kb on - strand at 609.592 kb on - strand at 610.151 kb on + strand, within RS_RS19505 at 610.152 kb on - strand, within RS_RS19505 at 610.271 kb on + strand, within RS_RS19505 at 610.271 kb on + strand, within RS_RS19505 at 610.271 kb on + strand, within RS_RS19505 at 610.271 kb on + strand, within RS_RS19505 at 610.272 kb on - strand, within RS_RS19505 at 610.635 kb on + strand, within RS_RS19505 at 610.635 kb on + strand, within RS_RS19505 at 610.636 kb on - strand, within RS_RS19505 at 610.636 kb on - strand, within RS_RS19505 at 610.647 kb on - strand, within RS_RS19505 at 610.705 kb on - strand, within RS_RS19505 at 610.776 kb on + strand, within RS_RS19505 at 610.776 kb on + strand, within RS_RS19505 at 610.776 kb on + strand, within RS_RS19505 at 610.776 kb on + strand, within RS_RS19505 at 610.776 kb on + strand, within RS_RS19505 at 610.776 kb on + strand, within RS_RS19505 at 610.776 kb on + strand, within RS_RS19505 at 610.777 kb on - strand, within RS_RS19505 at 610.777 kb on - strand, within RS_RS19505 at 610.777 kb on - strand, within RS_RS19505 at 610.777 kb on - strand, within RS_RS19505 at 610.777 kb on - strand, within RS_RS19505 at 610.777 kb on - strand, within RS_RS19505 at 611.054 kb on + strand at 611.054 kb on + strand at 611.060 kb on + strand at 611.060 kb on + strand at 611.060 kb on + strand at 611.060 kb on + strand at 611.060 kb on + strand at 611.060 kb on + strand at 611.061 kb on - strand at 611.061 kb on - strand at 611.170 kb on + strand at 611.170 kb on + strand at 611.171 kb on - strand at 611.171 kb on - strand at 611.171 kb on - strand at 611.171 kb on - strand at 611.359 kb on + strand, within RS_RS19510 at 611.359 kb on + strand, within RS_RS19510 at 611.359 kb on + strand, within RS_RS19510 at 611.530 kb on + strand, within RS_RS19510 at 611.531 kb on - strand, within RS_RS19510 at 611.686 kb on + strand, within RS_RS19510 at 611.686 kb on + strand, within RS_RS19510 at 611.707 kb on + strand, within RS_RS19510 at 611.707 kb on + strand, within RS_RS19510 at 611.776 kb on + strand at 611.777 kb on - strand at 611.777 kb on - strand at 611.826 kb on - strand at 611.826 kb on - strand at 612.039 kb on + strand at 612.040 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 609,375 + RS_RS19500 0.83 -0.8 609,375 + RS_RS19500 0.83 +0.5 609,487 + +0.2 609,487 + -0.7 609,487 + +0.4 609,488 - -0.2 609,488 - -0.1 609,488 - +0.8 609,488 - -2.0 609,591 + +0.8 609,592 - +0.1 609,592 - +0.1 610,151 + RS_RS19505 0.29 +0.6 610,152 - RS_RS19505 0.29 +1.5 610,271 + RS_RS19505 0.37 +0.8 610,271 + RS_RS19505 0.37 -0.9 610,271 + RS_RS19505 0.37 -0.8 610,271 + RS_RS19505 0.37 -0.1 610,272 - RS_RS19505 0.37 +0.8 610,635 + RS_RS19505 0.63 -0.4 610,635 + RS_RS19505 0.63 -1.0 610,636 - RS_RS19505 0.63 -0.4 610,636 - RS_RS19505 0.63 -0.4 610,647 - RS_RS19505 0.64 -0.5 610,705 - RS_RS19505 0.68 -0.2 610,776 + RS_RS19505 0.73 -0.2 610,776 + RS_RS19505 0.73 +0.1 610,776 + RS_RS19505 0.73 -0.1 610,776 + RS_RS19505 0.73 -0.3 610,776 + RS_RS19505 0.73 -0.9 610,776 + RS_RS19505 0.73 -1.0 610,776 + RS_RS19505 0.73 -0.2 610,777 - RS_RS19505 0.73 -0.1 610,777 - RS_RS19505 0.73 -0.2 610,777 - RS_RS19505 0.73 -0.7 610,777 - RS_RS19505 0.73 +0.2 610,777 - RS_RS19505 0.73 +0.1 610,777 - RS_RS19505 0.73 -0.8 611,054 + +1.1 611,054 + -0.1 611,060 + -0.8 611,060 + -0.5 611,060 + -0.7 611,060 + -0.8 611,060 + -0.8 611,060 + -0.5 611,061 - -0.2 611,061 - +0.2 611,170 + -0.2 611,170 + +0.0 611,171 - +0.1 611,171 - -0.5 611,171 - -1.0 611,171 - -0.7 611,359 + RS_RS19510 0.32 +0.4 611,359 + RS_RS19510 0.32 -0.1 611,359 + RS_RS19510 0.32 +0.5 611,530 + RS_RS19510 0.57 -0.6 611,531 - RS_RS19510 0.57 -0.4 611,686 + RS_RS19510 0.80 -0.3 611,686 + RS_RS19510 0.80 -0.1 611,707 + RS_RS19510 0.83 -0.2 611,707 + RS_RS19510 0.83 -0.4 611,776 + -0.8 611,777 - +0.3 611,777 - -0.2 611,826 - +0.2 611,826 - +0.3 612,039 + +0.4 612,040 - +0.8
Or see this region's nucleotide sequence