Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS19035 and RS_RS19040 overlap by 4 nucleotides RS_RS19040 and RS_RS19045 are separated by 35 nucleotides RS_RS19045 and RS_RS19050 overlap by 11 nucleotides
RS_RS19035: RS_RS19035 - flagellar basal body M-ring protein FliF, at 494,042 to 495,877
_RS19035
RS_RS19040: RS_RS19040 - flagellar motor switch protein FliG, at 495,874 to 496,866
_RS19040
RS_RS19045: RS_RS19045 - flagellar assembly protein FliH, at 496,902 to 497,648
_RS19045
RS_RS19050: RS_RS19050 - flagellum-specific ATP synthase FliI, at 497,638 to 499,083
_RS19050
Position (kb) 495
496
497 Strain fitness (log2 ratio) -2
-1
0
1 at 495.001 kb on + strand, within RS_RS19035 at 495.002 kb on - strand, within RS_RS19035 at 495.243 kb on - strand, within RS_RS19035 at 495.243 kb on - strand, within RS_RS19035 at 495.243 kb on - strand, within RS_RS19035 at 495.323 kb on + strand, within RS_RS19035 at 495.324 kb on - strand, within RS_RS19035 at 495.324 kb on - strand, within RS_RS19035 at 495.540 kb on - strand, within RS_RS19035 at 495.540 kb on - strand, within RS_RS19035 at 496.094 kb on - strand, within RS_RS19040 at 496.173 kb on + strand, within RS_RS19040 at 496.174 kb on - strand, within RS_RS19040 at 496.306 kb on + strand, within RS_RS19040 at 496.306 kb on + strand, within RS_RS19040 at 496.307 kb on - strand, within RS_RS19040 at 496.307 kb on - strand, within RS_RS19040 at 496.307 kb on - strand, within RS_RS19040 at 496.307 kb on - strand, within RS_RS19040 at 496.859 kb on - strand at 496.859 kb on - strand at 496.931 kb on + strand at 496.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 495,001 + RS_RS19035 0.52 +0.1 495,002 - RS_RS19035 0.52 +0.0 495,243 - RS_RS19035 0.65 -0.0 495,243 - RS_RS19035 0.65 -0.4 495,243 - RS_RS19035 0.65 -0.3 495,323 + RS_RS19035 0.70 -0.1 495,324 - RS_RS19035 0.70 +0.9 495,324 - RS_RS19035 0.70 +0.6 495,540 - RS_RS19035 0.82 -1.7 495,540 - RS_RS19035 0.82 -2.3 496,094 - RS_RS19040 0.22 +0.9 496,173 + RS_RS19040 0.30 +0.5 496,174 - RS_RS19040 0.30 -1.2 496,306 + RS_RS19040 0.44 -0.4 496,306 + RS_RS19040 0.44 +0.0 496,307 - RS_RS19040 0.44 -1.1 496,307 - RS_RS19040 0.44 -0.5 496,307 - RS_RS19040 0.44 -0.5 496,307 - RS_RS19040 0.44 +0.1 496,859 - -0.3 496,859 - +0.9 496,931 + +0.7 496,931 + +0.3
Or see this region's nucleotide sequence