Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS16775 and RS_RS16780 are separated by 149 nucleotides RS_RS16780 and RS_RS16785 are separated by 64 nucleotides RS_RS16785 and RS_RS16790 are separated by 74 nucleotides
RS_RS16775: RS_RS16775 - copper-translocating P-type ATPase, at 3,608,579 to 3,610,825
_RS16775
RS_RS16780: RS_RS16780 - copper chaperone, at 3,610,975 to 3,611,175
_RS16780
RS_RS16785: RS_RS16785 - OmpW family protein, at 3,611,240 to 3,611,965
_RS16785
RS_RS16790: RS_RS16790 - IS5 family transposase, at 3,612,040 to 3,612,864
_RS16790
Position (kb)
3610
3611
3612 Strain fitness (log2 ratio)
-2
-1
0
1 at 3610.257 kb on + strand, within RS_RS16775 at 3610.257 kb on + strand, within RS_RS16775 at 3610.258 kb on - strand, within RS_RS16775 at 3610.314 kb on + strand, within RS_RS16775 at 3610.314 kb on + strand, within RS_RS16775 at 3610.392 kb on + strand, within RS_RS16775 at 3610.524 kb on - strand, within RS_RS16775 at 3610.875 kb on + strand at 3610.876 kb on - strand at 3610.879 kb on - strand at 3610.898 kb on + strand at 3610.898 kb on + strand at 3610.898 kb on + strand at 3610.898 kb on + strand at 3610.930 kb on + strand at 3610.931 kb on - strand at 3610.931 kb on - strand at 3610.943 kb on + strand at 3610.943 kb on + strand at 3610.944 kb on - strand at 3610.944 kb on - strand at 3611.406 kb on + strand, within RS_RS16785 at 3611.407 kb on - strand, within RS_RS16785 at 3611.407 kb on - strand, within RS_RS16785 at 3611.407 kb on - strand, within RS_RS16785 at 3611.407 kb on - strand, within RS_RS16785 at 3611.538 kb on + strand, within RS_RS16785 at 3611.538 kb on + strand, within RS_RS16785 at 3611.538 kb on + strand, within RS_RS16785 at 3611.538 kb on + strand, within RS_RS16785 at 3611.539 kb on - strand, within RS_RS16785 at 3611.593 kb on - strand, within RS_RS16785 at 3611.593 kb on - strand, within RS_RS16785 at 3611.958 kb on + strand at 3611.959 kb on - strand at 3611.984 kb on - strand at 3611.984 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 3,610,257 + RS_RS16775 0.75 -0.5 3,610,257 + RS_RS16775 0.75 +0.3 3,610,258 - RS_RS16775 0.75 -0.0 3,610,314 + RS_RS16775 0.77 +0.1 3,610,314 + RS_RS16775 0.77 +0.6 3,610,392 + RS_RS16775 0.81 -0.1 3,610,524 - RS_RS16775 0.87 +0.6 3,610,875 + +0.5 3,610,876 - +0.2 3,610,879 - +0.4 3,610,898 + -0.5 3,610,898 + +0.4 3,610,898 + -0.1 3,610,898 + +0.1 3,610,930 + +0.4 3,610,931 - -2.1 3,610,931 - +0.7 3,610,943 + +0.3 3,610,943 + +0.1 3,610,944 - +1.2 3,610,944 - +0.3 3,611,406 + RS_RS16785 0.23 -0.1 3,611,407 - RS_RS16785 0.23 +0.1 3,611,407 - RS_RS16785 0.23 +0.2 3,611,407 - RS_RS16785 0.23 +0.2 3,611,407 - RS_RS16785 0.23 +0.5 3,611,538 + RS_RS16785 0.41 +0.3 3,611,538 + RS_RS16785 0.41 +0.4 3,611,538 + RS_RS16785 0.41 -0.2 3,611,538 + RS_RS16785 0.41 +0.3 3,611,539 - RS_RS16785 0.41 +0.1 3,611,593 - RS_RS16785 0.49 +0.5 3,611,593 - RS_RS16785 0.49 +0.7 3,611,958 + +0.2 3,611,959 - +0.2 3,611,984 - +1.2 3,611,984 - -0.9
Or see this region's nucleotide sequence